Home / Cyclin B2 / Human/Mouse Cyclin B2 Antibody

Human/Mouse Cyclin B2 Antibody

Catalog #: AF6004
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6004
AF6004-SP
Detection of Human and Mouse Cyclin B2 by Western Blot.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Cyclin B2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Cyclin B2 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Cyclin B2
Ala2-Leu101
Accession # P30276
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Cyclin B2 antibody by Western Blot. View Larger

Detection of Human and Mouse Cyclin B2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Cyclin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6004) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclin B2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyclin B2

Cyclin B2 (also CCNB2 and G2/mitotic-specific Cyclin B2) is a 48-54 kDa member of the cyclin AB subfamily, cyclin family of proteins. It is widely expressed and associates with CDK1. Here, it provides substrate specificity to a phosphorylating complex. A phosphor‑CDK1:Cyclin B2 complex is associated with the Golgi apparatus, where it contributes to Golgi fragmentation during mitosis. It is also possible that Cyclin B2 can substitute for Cyclin B1 during the early stages of mitosis. Human Cyclin B2 is 398 amino acids (aa) in length. It contains two cyclin box folds (aa 201‑290 and 298‑383) and two substrate binding sites (aa 165‑254 and 264‑347). Phosphorylation occurs at Ser92, Thr94, Ser99, Ser204, Ser392 and Ser398. Over aa 1‑101, human Cyclin B2 shares 79% aa identity with mouse Cyclin B2.

Entrez Gene IDs
9133 (Human); 12442 (Mouse); 363088 (Rat)
Alternate Names
CCNB2; Cyclin B2; G2/mitotic-specific cyclin-B2; HsT17299

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Citations for Human/Mouse Cyclin B2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Mis12 controls cyclin B1 stabilization via Cdc14B-mediated APC/CCdh1 regulation during meiotic G2/M transition in mouse oocytes
    Authors: Guang-Yu Bai, Min Ho Choe, Jae-Sung Kim, Jeong Su Oh
    Development
  2. Alternative cleavage and polyadenylation of the Ccnb1 mRNA defines accumulation of cyclin protein during the meiotic cell cycle
    Authors: Wang, X;Leung, FS;Bush, JO;Conti, M;
    Nucleic acids research
    Species: Mouse
    Sample Types: Oocytes
    Applications: Western Blot
  3. CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I
    Authors: CJ Zhou, XY Wang, YH Dong, DH Wang, Z Han, XJ Zhang, QY Sun, J Carroll, CG Liang
    Nature Communications, 2022-12-13;13(1):7732.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. The cohesin stabilizer Sororin drives G2-M transition and spindle assembly in mammalian oocytes
    Authors: C Zhou, X Zhang, Y Miao, Y Zhang, Y Li, B Xiong
    Science Advances, 2021-09-24;7(39):eabg9335.
    Species: Mouse
    Sample Types: Oocytes
    Applications: Western Blot
  5. Genome-wide analysis reveals a switch in the translational program upon oocyte meiotic resumption
    Authors: XG Luong, EM Daldello, G Rajkovic, CR Yang, M Conti
    Nucleic Acids Res., 2020-04-06;48(6):3257-3276.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  6. The Translation of Cyclin B1 and B2 is Differentially Regulated during Mouse Oocyte Reentry into the Meiotic Cell Cycle
    Authors: SJ Han, JPS Martins, Y Yang, MK Kang, EM Daldello, M Conti
    Sci Rep, 2017-10-26;7(1):14077.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis.
    Authors: Muruganandan S, Parlee SD, Rourke JL, Ernst MC, Goralski KB, Sinal CJ
    J. Biol. Chem., 2011-05-14;286(27):23982-95.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Cyclin B2 is required for progression through meiosis in mouse oocytes
    Authors: Enrico Maria Daldello, Xuan G. Luong, Cai-Rong Yang, Jonathan Kuhn, Marco Conti
    Development

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