Human/Mouse CX3CR1 Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse CX3CR1 by Western Blot. Western blot shows lysates of M1 mouse myeloid leukemia cell line and NK-92 human natural killer lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CX3CR1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of CX3CR1 in M1 Mouse Cell Line by Flow Cytometry. M1 mouse myeloid leukemia cell line was stained with Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: CX3CR1
CX3CR1 (CX 3 C chemokine receptor 1; also fractalkine receptor, GPCR13 and V28) is a 40 kDa (predicted) member of the δ-group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1 lo monocytes plus smooth muscle and mast cells. CX 3 CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1-32, 91-104, 169-196 and 258-274 collectively, mouse CX 3 CR1 shares 96% and 77% aa identity with rat and human CX 3 CR1, respectively.
Product Datasheets
Citations for Human/Mouse CX3CR1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Anti-Inflammatory Neutrophils Reprogram Macrophages toward a Pro-Healing Phenotype with Increased Efferocytosis Capacity
Authors: Mihaila, AC;Ciortan, L;Tucureanu, MM;Simionescu, M;Butoi, E;
Cells
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Novel adherent CD11b+Gr-1+tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment
Authors: T Tsubaki, T Kadonosono, S Sakurai, T Shiozawa, T Goto, S Sakai, T Kuchimaru, T Sakamoto, H Watanabe, G Kondoh, S Kizaka-Kon
Oncotarget, 2018-01-29;9(13):11209-11226.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Migration-based selections of antibodies that convert bone marrow into trafficking microglia-like cells that reduce brain amyloid ?
Authors: KH Han, BM Arlian, MS Macauley, JC Paulson, RA Lerner
Proc. Natl. Acad. Sci. U.S.A., 2018-01-02;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Hepatectomy leads to loss of TRAIL-expressing liver NK cells via downregulation of the CXCL9-CXCR3 axis in mice
Authors: T Yano, M Ohira, R Nakano, Y Tanaka, H Ohdan
PLoS ONE, 2017-10-31;12(10):e0186997.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
A CCR2+ myeloid cell niche required for pancreatic ? cell growth
Authors: K Mussar, S Pardike, TM Hohl, G Hardiman, V Cirulli, L Crisa
JCI Insight, 2017-08-03;2(15):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
MicroRNA-101a regulates microglial morphology and inflammation
Authors: R Saika, H Sakuma, D Noto, S Yamaguchi, T Yamamura, S Miyake
J Neuroinflammation, 2017-05-30;14(1):109.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Selective suppression of the ? isoform of p38 MAPK rescues late-stage tau pathology
Alzheimers Res Ther, 2016-12-15;8(1):54.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
MKEY, a Peptide Inhibitor of CXCL4-CCL5 Heterodimer Formation, Protects Against Stroke in Mice
J Am Heart Assoc, 2016-09-15;5(9):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
Authors: Kowalewska P, Patrick A, Fox-Robichaud A
PLoS ONE, 2014-09-03;9(9):e104537.
Species: Mouse
Sample Types: In Vivo
Applications: IHC-Fr -
Fractalkine is a novel human adipochemokine associated with type 2 diabetes.
Authors: Shah R, Hinkle CC, Ferguson JF, Mehta NN, Li M, Qu L, Lu Y, Putt ME, Ahima RS, Reilly MP
Diabetes, 2011-05-01;60(5):1512-8.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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