Human/Mouse Bad aa 1‑21 Antibody

Name: Human/Mouse Bad aa 1‑21 Antibody
Brand: R&D Systems
SKU: AF819
Rating: 4 (2 reviews)

Catalog #: AF819
4 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF819
AF819-SP

Detection of Human/Mouse Bad by Western Blot.

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Product Details

Citations (4)

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Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Bad aa 1‑21 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Bad in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

KLH-coupled human Bad synthetic peptide
MFQIPEFEPSEQEDSSSAERGC
Accession # Q92934

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

0.3-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse Bad by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and L-929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse Bad Antigen Affinity-purified Polyclonal Antibody (Catalog # AF819) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bad at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry

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Bad in Jurkat Human Cell Line. Bad was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Rabbit Anti-Human/Mouse Bad Antigen Affinity-purified Polyclonal Antibody (Catalog # AF819) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bad

Bcl-2 antagonist of cell death (BAD) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of BAD on Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Over amino acid 1-21, human BAD shares 81 % aa sequence identity with mouse and rat BAD.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Entrez Gene IDs

572 (Human); 12015 (Mouse); 64639 (Rat)

Alternate Names

Bad; BBC2; BBC6; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; Bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; BCL2L8; Bcl2-L-8; BCL2L8bcl2-L-8; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-XL/Bcl-2-associated death promoter

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse Bad aa 1‑21 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers
Authors: Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J;
Biosensors
Species: Mouse
Sample Types: Complex Sample Type
Applications: IHC
TCDD promotes lung tumors via attenuation of apoptosis through activation of the Akt and ERK1/2 signaling pathways.
Authors: Chen R, Siao S, Hsu C, Chang C, Chang L, Wu C, Lin P, Wang Y
PLoS ONE, 2014-06-13;9(6):e99586.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay
Sustained early growth response gene 3 expression inhibits the survival of CD4/CD8 double-positive thymocytes.
Authors: Xi H, Kersh GJ
J. Immunol., 2004-07-01;173(1):340-8.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot
Translocation of full-length Bid to mitochondria during anoikis.
Authors: Valentijn AJ, Gilmore AP
J. Biol. Chem., 2004-05-17;279(31):32848-57.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot