Human MFAP3 APC-conjugated Antibody

Catalog #: FAB113251A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB113251A-100UG

Detection of MFAP3 in HEK293 cells transfected with Human MFAP-3L and eGFP cells by Flow Cytometry.

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Human MFAP3 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MFAP3L in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1059143

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human MFAP3L
Met1-Met149
Accession # O75121

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

HEK293 cells transfected with Human MFAP-3L and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MFAP3 in HEK293 cells transfected with Human MFAP-3L and eGFP cells by Flow Cytometry. HEK293 cells transfected with Human MFAP-3L and eGFP were stained with eGFP and either (A) Mouse Anti-Human MFAP3 APC‑conjugated Monoclonal Antibody (Catalog # FAB113251A) or (B) Mouse IgG_2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MFAP3

Microbibrillar-Associated Protein 3-Like (MFAP3L), also known as NYD-sp9, is part of the microfibrillar-associated protein family (MFAPs). MFAPs are non‐fibrillin, extracellular matrix glycoproteins that interact with fibrillin and were originally characterized in microfibrillar assembly (1, 2). In humans, there several subfamily members with varying amino acid (aa) sequence homology and functions (1, 2). Among the family, MFAP2 and MFAP5 are more closely related and while MFAP1, 3 and 4 share no structural or sequence homology with MFAP2, MFAP5 or with each other (1, 2). Human MFAP3L shows 71% amino acid (aa) sequence homology to MFAP3, but not other MFAPs (3). Mature, human MFAP3L consists of an extracellular domain (ECD) containing N-linked glycosylation sites, a transmembrane domain, and a cytoplasmic domain with a conserved SH2 motif (3). The ECD of human MFAP3L shares 89% and 90% aa sequence identity with mouse and rat MFAP3L, respectively. MFAPs have the unique ability to interact with TGF-beta family growth factors, Notch and Notch ligands and multiple elastic fiber proteins, in addition to interacting with fibrillin (1, 2). MFAPs are expressed in a wide variety of tissues and, along with microfibril assembly, they play roles in the regulation of tissue homeostasis, cell survival, and tumor progression (1, 2). MFAP3L is often located within colorectal cancer (CRC) cells, which metastasize by activation of the nuclear ERK pathway via MFAP3L phosphorylation (3). Regulation of this MFAP3L activity could have pharmaceutical effects on CRC tumor progression (3).

References