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Human M-CSF R/CD115 Antibody

Catalog #: MAB3291
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3291-SP
MAB3291-100
MAB3291-500
Detection of Human M-CSF R/CD115 by Western Blot.
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Citations (11)
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Human M-CSF R/CD115 Antibody Summary

Species Reactivity
Human
Specificity
Detects human M-CSF R/CD115 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or rhGM-CSF R beta is observed.
Source
Monoclonal Mouse IgG1 Clone # 61701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human M-CSF R/CD115
Ile20-Glu512 (Pro54Ala)
Accession # P07333.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of Human Spleen
Neutralization
Measured by its ability to neutralize M-CSF R/CD115-mediated inhibition of proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. The Neutralization Dose (ND50) is typically 4-16 ng/mL in the presence of 30 ng/mL Recombinant Human M-CSF R/CD115 Fc Chimera and 1 ng/mL Recombinant Human
M-CSF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human M-CSF R/CD115 antibody by Western Blot. View Larger

Detection of Human M-CSF R/CD115 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for M-CSF R/CD115 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization M-CSF R/CD115 Inhibition of M-CSF-dependent Cell Proliferation and Neutralization by Human M-CSF R/CD115 Antibody. View Larger

M-CSF R/CD115 Inhibition of M-CSF-dependent Cell Proliferation and Neutralization by Human M-CSF R/CD115 Antibody. Recombinant Human M-CSF R/CD115 Fc Chimera (329-MR) inhibits Recombinant Human M-CSF (Catalog # 216-MC) induced proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human M-CSF R/CD115 (1 ng/mL) activity elicited by Recombinant Human M-CSF R/CD115 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291). The ND50 is typically 4-16 ng/mL.

Immunohistochemistry View Larger

Detection of M-CSF R/CD115 in Human Spleen. M-CSF R/CD115 was detected in immersion fixed paraffin-embedded sections of Human Spleen using Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-CSF R/CD115

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75%, and 76% aa identity with mouse, rat, bovine, canine, feline, and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK, and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts, and DC (3, 4). M-CSF R and integrin  alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

References
  1. deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
  2. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
  3. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  4. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
  5. Rovida, E. et al. (2001) J. Immunol. 166:1583.
  6. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
  7. Dai, X. et al. (2002) Blood 99:111.
  8. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  9. Li, M. et al. (2004) J. Neurochem. 91:623.
  10. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.
Long Name
Macrophage Colony Stimulating Factor Receptor
Entrez Gene IDs
1436 (Human); 12978 (Mouse)
Alternate Names
CD115 antigen; CD115; c-fms; colony stimulating factor 1 receptor; CSF1R; CSF-1-R; CSFR; EC 2.7.10.1; FMS proto-oncogene; FMSFIM2; macrophage colony stimulating factor I receptor; macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; M-CSF R; MCSFR; M-CSFR; Proto-oncogene c-Fms

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Citations for Human M-CSF R/CD115 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Age-related changes in brain phospholipids and bioactive lipids in the APP knock-in mouse model of Alzheimer's disease
    Authors: C Emre, KV Do, B Jun, E Hjorth, SG Alcalde, MI Kautzmann, WC Gordon, P Nilsson, NG Bazan, M Schultzber
    Acta neuropathologica communications, 2021-06-29;9(1):116.
  2. An adapted protocol to derive microglia from stem cells and its application in the study of CSF1R-related disorders
    Authors: Dorion, MF;Casas, D;Shlaifer, I;Yaqubi, M;Fleming, P;Karpilovsky, N;Chen, CX;Nicouleau, M;Piscopo, VEC;MacDougall, EJ;Alluli, A;Goldsmith, TM;Schneider, A;Dorion, S;Aprahamian, N;MacDonald, A;Thomas, RA;Dudley, RWR;Hall, JA;Fon, EA;Antel, JP;Stratton, JA;Durcan, TM;La Piana, R;Healy, LM;
    Molecular neurodegeneration
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Extracellular vesicles from triple negative breast cancer promote pro-inflammatory macrophages associated with better clinical outcome
    Authors: M Tkach, J Thalmensi, E Timperi, P Gueguen, N Névo, E Grisard, P Sirven, F Cocozza, A Gouronnec, L Martin-Jau, M Jouve, F Delisle, N Manel, DC Rookhuizen, CL Guerin, V Soumelis, E Romano, E Segura, C Théry
    Proceedings of the National Academy of Sciences of the United States of America, 2022-04-19;119(17):e2107394119.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. Phenotype of vigilin expressing breast cancer cells binding to the 69 nt 3'UTR element in CSF-1R mRNA
    Authors: HH Woo, SC Lee, JB Stoffer, D Rush, SK Chambers
    Transl Oncol, 2018-10-03;12(1):106-115.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. IL-34 Upregulated Th17 Production through Increased IL-6 Expression by Rheumatoid Fibroblast-Like Synoviocytes
    Authors: B Wang, Z Ma, M Wang, X Sun, Y Tang, M Li, Y Zhang, F Li, X Li
    Mediators Inflamm., 2017-06-04;2017(0):1567120.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Receptor-Type Protein-Tyrosine Phosphatase ? and Colony Stimulating Factor-1 Receptor in the Intestine: Cellular Expression and Cytokine- and Chemokine Responses by Interleukin-34 and Colony Stimulating Factor-1
    PLoS ONE, 2016-11-29;11(11):e0167324.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Autocrine CSF1R signaling mediates switching between invasion and proliferation downstream of TGFbeta in claudin-low breast tumor cells.
    Authors: Patsialou A, Wang Y, Pignatelli J, Chen X, Entenberg D, Oktay M, Condeelis J
    Oncogene, 2014-08-04;34(21):2721-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Macrophage colony-stimulating factor augments Tie2-expressing monocyte differentiation, angiogenic function, and recruitment in a mouse model of breast cancer.
    Authors: Forget M, Voorhees J, Cole S, Dakhlallah D, Patterson I, Gross A, Moldovan L, Mo X, Evans R, Marsh C, Eubank T
    PLoS ONE, 2014-06-03;9(6):e98623.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Derepression of an endogenous long terminal repeat activates the CSF1R proto-oncogene in human lymphoma.
    Authors: Lamprecht B, Walter K, Kreher S, Kumar R, Hummel M, Lenze D, Kochert K, Bouhlel MA, Richter J, Soler E, Stadhouders R, Johrens K, Wurster KD, Callen DF, Harte MF, Giefing M, Barlow R, Stein H, Anagnostopoulos I, Janz M, Cockerill PN, Siebert R, Dorken B, Bonifer C, Mathas S
    Nat. Med., 2010-05-02;16(5):571-9.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation
  10. Invasive breast carcinoma cells from patients exhibit Mena INV - and macrophage-dependent transendothelial migration
    Authors: Jeanine Pignatelli, Sumanta Goswami, Joan G. Jones, Thomas E. Rohan, Evan Pieri, Xiaoming Chen et al.
    Science Signaling
  11. Osteopontin Links Myeloid Activation and Disease Progression in Systemic Sclerosis
    Authors: Xia Gao, Guiquan Jia, Anna Guttman, Daryle J. DePianto, Katrina B. Morshead, Kai-Hui Sun et al.
    Cell Reports Medicine

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