Human Lysyl Oxidase Homolog 2 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: Lysyl Oxidase Homolog 2/LOXL2
Lysyl Oxidase Homolog 2 is one of several secreted copper-binding amine oxidases that oxidize primary amine substrates to aldehydes. It is widely expressed, particularly in placenta, uterus, and prostate. LOXL2 is upregulated during cancer progression and promotes cell migration and tumor cell invasiveness.
Citations for Human Lysyl Oxidase Homolog 2 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Increased serum LOXL2 concentration in pelvic inflammatory disease with pelvic adhesion
Authors: C Xie, B Tang, K Wu, Q Meng, F Wang
Oncogene, 2022-03-04;22(1):59.
Species: Human
Sample Types: Serum
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Adiponectin and the steatosis marker Chi3L1 decrease following switch to raltegravir compared to continued PI/NNRTI-based antiretroviral therapy
Authors: O Offor, N Utay, D Reynoso, A Somasunder, J Currier, J Lake
PLoS ONE, 2018-05-10;13(5):e0196395.
Species: Human
Sample Types: Plasma
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Circulating LOXL2 Levels Reflect Severity of Intestinal Fibrosis and GALT CD4(+) T Lymphocyte Depletion in Treated HIV Infection
Authors: S Seang, A Somasunder, M Nigalye, M Somsouk, TW Schacker, JL Sanchez, PW Hunt, NS Utay, JE Lake
Pathog Immun, 2017-06-21;2(2):239-252.
Species: Human
Sample Types: Plasma
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