Human LILRA5/CD85f Antibody

Catalog #: AF6754 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6754
AF6754-SP

Detection of LILRA5/CD85f in Human peripheral blood monocytes by Flow Cytometry.

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All LILRA5/CD85f Antibodies

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Human LILRA5/CD85f Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRA5/CD85f in direct ELISAs.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human LILRA5/CD85f
Gly42-Arg268
Accession # A6NI73

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA binding assay, 1-7 μg/mL of this antibody will block 50% of the binding of 1 μg/mL of Recombinant Human Angiopoietin-like 7 (Catalog # 914-AN) to immobilized Recombinant Human LILRA5/CD85f/LIR-9 (Catalog # 8956-T4) coated at 2 µg/mL (100 µL/well). At 15 µg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LILRA5/CD85f in Human peripheral blood monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Sheep Anti-Human LILRA5/CD85f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6754, filled histogram) or control antibody (5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA5/CD85f

ILT11 (leukocyte immunoglobulin-like receptor 9; also CD85f, LIR-9 and LILRA5) is a 39-40 kDa Group 2 member of the LILR family of innate immune receptors. It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of proinflammatory cytokines such as TNF-alpha and IL-1 beta. Mature human LIR-9 is a 258 amino acid (aa) type I transmembrane glycoprotein. It has a 227 aa extracellular domain (aa 42-268) that contains two C2-type Ig-like domains
(aa 51‑136 and 142-230), and a 10 aa cytoplasmic tail. LIR-9 has three potential splice forms. One is a 35 kDa soluble form of the molecule described above that shows a 27 aa substitution for aa 239-299. The other two splice forms are analogous to the above membrane and soluble forms, but demonstrate signal sequence cleavage further downstream after Ala51. Over aa 41‑268, human LIR-9 shares 58% aa identity with mouse LIR-9.

Long Name

Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5

Entrez Gene IDs

353514 (Human); 232801 (Mouse); 691533 (Rat)

Alternate Names

CD85 antigen-like family member F; CD85; CD85f antigen; CD85f; ILT11; ILT-11; ILT11CD85f; Immunoglobulin-like transcript 11; leukocyte Ig-like receptor 9; Leukocyte immunoglobulin-like receptor 9; leukocyte immunoglobulin-like receptor subfamily A member 5 soluble; leukocyte immunoglobulin-like receptor subfamily A member 5; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRA5; LILRB7; LIR9; LIR-9; LIR9immunoglobulin-like transcript 11 protein; member 7