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Human Leukotriene B4 R1 PE-conjugated Antibody

Catalog #: FAB099P
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB099P
Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry.
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Citations (3)
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Human Leukotriene B4 R1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Recognizes human Leukotriene B4 R1 (5).
Source
Monoclonal Mouse IgG1 Clone # 203/14F11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HeLa cervical epithelial carcinoma cell line transfected with human Leukotriene B4 R1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Leukotriene B4 R1 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Leukotriene B4 R1 PE-conjugated Monoclonal Antibody (Catalog # FAB099P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Leukotriene B4 R1

Polymorphonuclear granulocytes secrete the lipid chemotactic mediator Leukotriene B4 (LTB4) in response to inflammatory stimuli (1). Neutrophils, monocytes and lymphocytes respond to LTB4 via specific receptors localized on the cell surface (2-4). The high affinity LTB4 Receptor known as BLT1 is only expressed on leukocytes (5-7) while a second low affinity receptor BLT2 is expressed more ubiquitously (8, 9). The BLT1 and BLT2 are G-protein linked seven-transmembrane spanning receptors that share about 37-45% amino acid identity (8, 9). Enhanced LTB4 production and engagement of the BLT receptors can be important in allergic and inflammatory diseases such as asthma (10), allergic encephalomyelitis (11), endotoxic shock (12), ischemia (12), psoriasis (13), rheumatoid arthritis (14) and inflammatory bowel disease (15). In addition, it has been reported that BLT1 can function as an additional co-receptor for HIV infection of CD4+ T cells (16, 17). Investigations into the mechanisms and potential inhibitors of LTB4 binding to its’ receptors may provide insight into possible treatment modalities for a number of inflammatory disorders.

References
  1. Samuelsson, B. et al. (1987) Science 237:1171.
  2. Brom, J. and W. Konig (1989) Immunology 68:479.
  3. Patry, C. et al. (1996) Prostaglandins, Leukotrienes and Essential Fatty Acids 54:361.
  4. Dasari, V.R. et al. (2000) Immunopharm. 48:157.
  5. Pettersson, A. et al. (2000) Biochem. Biophys. Res. Comm. 279:520.
  6. Yokomizo, T. et al. (1997) Nature 387:620.
  7. Kato, K. et al. (2000) J. Exp. Med. 192:413.
  8. Yokomizo, T. et al. (2000) J. Exp. Med. 192:421.
  9. Kamohara, M. et al. (2000) J. Biol. Chem. 275:27000.
  10. Turner, C.R. et al. (1996) J. Clin. Invest. 97:381.
  11. Gladue, R.P. et al. (1996) J. Exp. Med. 183:1893.
  12. Lefer, A.M. (1986) Biochem. Pharmacol. 35:123.
  13. Iverson, L. et al. (1997) Skin Pharmacol. 10:169.
  14. Griffiths, R.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:517.
  15. Sharon, P. and W.F. Stenson (1984) Gastroenterology 86:453.
  16. Owman, C. et al. (1996) Proc. Natl. Acad. Sci. USA 95:9530.
  17. Martin, V. et al. (1999) J. Biol. Chem. 274:8597.
Long Name
Leukotriene B4 Receptor 1
Entrez Gene IDs
1241 (Human)
Alternate Names
BLT1; chemokine receptor-like 1; CMKRL1; Leukotriene B4 R1; leukotriene B4 receptor; Leukotriene B4R1; LTB4R; LTB4R1; LTBR1; P2Y purinoceptor 7; purinergic receptor P2Y, G-protein coupled, 7

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Citations for Human Leukotriene B4 R1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Leukotriene B4 promotes neovascularization and macrophage recruitment in murine wet-type AMD models
    Authors: F Sasaki, T Koga, M Ohba, K Saeki, T Okuno, K Ishikawa, T Nakama, S Nakao, S Yoshida, T Ishibashi, H Ahmadieh, MR Kanavi, A Hafezi-Mog, JM Penninger, KH Sonoda, T Yokomizo
    JCI Insight, 2018-09-20;3(18):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Expression of enzymes and receptors of the leukotriene pathway in human neuroblastoma promotes tumor survival and provides a target for therapy.
    Authors: Sveinbjornsson B, Rasmuson A, Baryawno N, Wan M, Pettersen I, Ponthan F, Orrego A, Haeggstrom JZ, Johnsen JI, Kogner P
    FASEB J., 2008-06-30;22(10):3525-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Residues from transmembrane helices 3 and 5 participate in leukotriene B4 binding to BLT1.
    Authors: Sabirsh A, Bywater RP, Bristulf J, Owman C, Haeggstrom JZ
    Biochemistry, 2006-05-09;45(18):5733-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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