Human LAT Antibody Summary
His30-Gln113
Accession # O43561
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human LAT by Western Blot. Western Blot shows lysates of HEL 92.1.7 human erythroleukemic cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human LAT Monoclonal Antibody (Catalog # MAB63341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for LAT at approximately 36, 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAT
LAT (Linker for activation of T cells; also pp36) is a 36-38 kDa type III (no signal sequence; extracellular N-terminus) transmembrane adaptor phosphoprotein. It is expressed on mast cells, NK cells, monocytes (but not macrophages), platelets and T cells. LAT is phosphorylated upon ligation of the TCR, GPVI, and Fc epsilon RI, and serves as an anchor for several SH2-domain containing signaling proteins, including PLCg1, SLP76 and Grb2. Human LAT is 262 amino acids (aa) in length. It contains a four aa extracellular segment with a 235 aa cytoplasmic domain (aa 28-262). Palmitoylation occurs on Cys26 and 29. There are 13 Ser/Thr phosphorylation sites plus five Tyr sites. Multiple potential splice variants are reported. There is an alternative start site 36 aa upstream of the standard site that may be accompanied by a deletion of aa 114-142. There is also a deletion of aa 88-97, and three substitutions: two aa for aa 215-262, 38 aa for aa 55-262, and 50 aa for aa 35-262. Over aa 30-113, human LAT shares 73% aa identity with mouse LAT.
Product Datasheets
Citation for Human LAT Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation
Authors: JM Carpier, AE Zucchetti, L Bataille, S Dogniaux, M Shafaq-Zad, S Bardin, M Lucchino, M Maurin, LD Joannas, JG Magalhaes, L Johannes, T Galli, B Goud, C Hivroz
J. Exp. Med., 2018-02-12;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC
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