Home / KLF2 / Human KLF2 Antibody

Human KLF2 Antibody

Catalog #: MAB5466
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5466
MAB5466-SP
KLF2 in BG01V Human Cells.
2 Images
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KLF2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human KLF2 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) KLF17 and no cross-reactivity with rhKLF1, 4, 5, 6, 10, 12, 17, recombinant mouse KLF4 or 15 is observed.
Source
Monoclonal Mouse IgG2B Clone # 665333
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human KLF2
Pro71-Pro168 (predicted)
Accession # Q9Y5W3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry KLF2 antibody in BG01V Human Cells by Immunocytochemistry (ICC). View Larger

KLF2 in BG01V Human Cells. KLF2 was detected in immersion fixed BG01V human embryonic stem cells using Human KLF2 Monoclonal Antibody (Catalog # MAB5466) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of KLF2 antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of KLF2 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human KLF2 Monoclonal Antibody (Catalog # MAB5466, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLF2

KLF2 (Kruppel-like factor 2; also LKLF) is a lung-associated, 37 kDa member of the Kruppel C2H2-type zinc-finger protein family.  KLF2 is found in airway epithelium, endothelium, monocytes, T and B cells.  It is a transcription factor that regulates multiple genes, many of which are involved in cell migration.   Human KLF2 is 355 amino acids (aa) in length.  It contains an activation domain (aa 1-110), an inhibitory domain (aa 111-267), and three C2H2-type zinc-finger regions (aa 272-354).  There is one potential splice form that shows a premature truncation after Asp224.  Over aa 71-168, human KLF2 is 82% aa identical to mouse KLF2.  

Long Name
Kruppel-Like Factor 2
Entrez Gene IDs
10365 (Human); 16598 (Mouse); 306330 (Rat)
Alternate Names
KLF2; Krueppel-like factor 2; Kruppel-like factor 2 (lung); Kruppel-like factor LKLF; LKLF; LKLFLung krueppel-like factor; lung Kruppel-like zinc finger transcription factor

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human KLF2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Inactivation of Cerebral Cavernous Malformation Genes Results in Accumulation of von Willebrand Factor and Redistribution of Weibel-Palade Bodies in Endothelial Cells
    Authors: Christiane D. Much, Barbara S. Sendtner, Konrad Schwefel, Eric Freund, Sander Bekeschus, Oliver Otto et al.
    Frontiers in Molecular Biosciences
  2. Memantine protects against ischemia/reperfusion-induced brain endothelial permeability
    Authors: Y Liu, Y Huang, Y Xu, P Qu, M Wang
    IUBMB Life, 2018-03-15;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Cellular origin and pathophysiology of chronic lymphocytic leukemia.
    Authors: Seifert M, Sellmann L, Bloehdorn J, Wein F, Stilgenbauer S, Durig J, Kuppers R
    J Exp Med, 2012-10-22;209(12):2183-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Renoprotective Effect of KLF2 on Glomerular Endothelial Dysfunction in Hypertensive Nephropathy
    Authors: Eunjin Bae, Mi-Yeon Yu, Jong-Joo Moon, Ji-Eun Kim, Saram Lee, Sang-Woong Han et al.
    Cells

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human KLF2 Antibody

There are currently no reviews for this product. Be the first to review Human KLF2 Antibody and earn rewards!

Have you used Human KLF2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review