Human IRAK1 Antibody

Detection of Human IRAK1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human IRAK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4048) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, recombinant human IRAK1, IRAK2, and IRAK4 (10 ng/lane) were included. A specific band for IRAK1 was detected at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human IRAK1 by Simple Western^TM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for IRAK1 at approximately 83 kDa (as indicated) using 20 µg/mL of Goat Anti-Human IRAK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4048). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.