Human Integrin beta 6 Antibody Summary
Gly22-Asn707
Accession # P18564
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Integrin beta 6 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Sheep Anti-Human Integrin beta 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4155, filled histogram) or isotype control antibody (5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
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Integrin beta 6 in HT‑29 Human Cell Line. Integrin beta 6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Sheep Anti-Human Integrin beta 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4155) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Integrin beta 6 in HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells. Integrin beta 6 was detected in immersion fixed HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and A549 Human Lung Carcinoma Cell Line (Negative) Cells using Sheep Anti-Human Integrin beta 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4155) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 6
Integrin beta 6 (ITG beta 6) is a 95 kDa member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha V and appears on epithelia following injury or inflammation. It activates TGF-beta and assists keratinocyte migration. Human Integrin beta 6 is a type I transmembrane glycoprotein that is 767 aa in length. It contains a 688 aa extracellular domain (ECD) (aa 22‑709) that incorporates a 241 aa VWF-A domain. Over aa 22‑707, human integrin beta 6 ECD shares 90% and 93% aa sequence identity with mouse and pig integrin beta 6 ECD, respectively.
Product Datasheets
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