Human Integrin  alpha V beta 5 Antibody

Integrin alpha V beta 5 in HT1080 Human Cell Line. Integrin aV beta 5 was detected in immersion fixed HT1080 human fibrosarcoma cell line using Human Integrin aV beta 5 Monoclonal Antibody (Catalog # MAB2528) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Integrin alpha V beta 5 by Immunocytochemistry/Immunofluorescence alpha V beta 3 and EGFR mediate MBsome signaling. a–d HeLa cells were incubated with purified GFP-MBs for 3 h, followed by wash and another incubation for 24 h. Cells were then fixed and stained with anti-alpha V beta 3 (a), anti-alpha V beta 5 (b), anti-phospho-FAK (d) antibodies. Panels in c show control staining where primary antibodies were not added. Boxed regions mark the part of the image shown as a higher magnification image in the insets on the right. Scale bar is equivalent to 1 μm. e HeLa cells were co-incubated with purified GFP-MBs and EGF-Alexa647, followed by wash and another incubation for 24 h. Cells were then fixed and colocalization between MBs and EGF analyzed. Arrows point to MBsomes. f HeLa cells were co-incubated with purified GFP-MBs and non-labeled EGF, followed by wash and another incubation for 24 h. Cells were then fixed and stained with anti-phospho-EGFR antibodies. Arrows point to MBsomes. Scale bars in insets are equivalent to 2 μm. g HeLa cells were incubated with purified GFP-MBs. Cells were then washed and flow sorted to separate fractions with or without internalized GF-MBs. Equal number of cells from each fraction were then plated and incubated for 48 h in the presence or absence of 10 μm of EGFR inhibitor (erlotinib). Cells were then washed again and incubated for another 48 h followed by cell counting to determine the number of cells. Data shown are the means and standard deviations derived from three independent experiments (one-way ANOVA) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31320617), licensed under a CC-BY license. Not internally tested by R&D Systems.