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Human Integrin alpha V beta 5 Antibody

Catalog #: MAB2528
13 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2528
MAB2528-SP
Integrin alpha V beta 5 in HT1080 Human Cell Line.
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Product Details
Citations (13)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin alpha V beta 5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha V beta 5. Recognizes the human Integrin alpha V beta 5 heterodimer and does not recognize the alpha V subunit in association with any other beta subunits.
Source
Monoclonal Mouse IgG1 Clone # P5H9
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HT1080 human fibrosarcoma cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
MCF‑7 human breast cancer cell line
Immunoprecipitation
Wayner, E.A. et al. (1991) J. Cell Biol. 113:919.
 
Adhesion Blockade
Wayner, E.A. et al. (1991) J. Cell Biol. 113:919.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Integrin aV beta 5 antibody in HT1080 Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin alpha V beta 5 in HT1080 Human Cell Line. Integrin aV beta 5 was detected in immersion fixed HT1080 human fibrosarcoma cell line using Human Integrin aV beta 5 Monoclonal Antibody (Catalog # MAB2528) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Integrin alpha V beta 5

Integrins are heterodimeric receptors comprised of an alpha and a beta subunit. Integrin alpha V (CD51) associates with several different beta subunits, but Integrin beta 5 associates exclusively with the alpha V subunit. Integrin alpha V beta 5, also known as Integrin alpha V beta S and Integrin alpha V beta 3B, is a transmembrane heterodimeric protein that functions as a receptor for Vitronectin. It is expressed on hepatoma cells, fibroblasts and carcinoma cells.

Entrez Gene IDs
3685 (Human)
Alternate Names
CD51; Integrin alpha V beta 5; integrin subunit alpha V; ITGAV; MSK8; VNRA; VTNR

Product Datasheets

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Citations for Human Integrin alpha V beta 5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
    Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
    Nature Immunology, 2022-12-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Direct targeting of oncogenic RAS mutants with a tumor-specific cytosol-penetrating antibody inhibits RAS mutant-driven tumor growth
    Authors: SM Shin, JS Kim, SW Park, SY Jun, HJ Kweon, DK Choi, D Lee, YB Cho, YS Kim
    Sci Adv, 2020-01-15;6(3):eaay2174.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The oncolytic adenovirus VCN-01 promotes anti-tumor effect in primitive neuroectodermal tumor models
    Authors: M Garcia-Mou, N Martinez-V, M Gonzalez-H, L Marrodán, M Cascallo, R Alemany, A Patiño-Gar, MM Alonso
    Sci Rep, 2019-10-07;9(1):14368.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The post-abscission midbody is an intracellular signaling organelle that regulates cell proliferation
    Authors: E Peterman, P Gibieža, J Schafer, VA Skeberdis, A Kaupinis, M Valius, X Heiligenst, I Hurbain, G Raposo, R Prekeris
    Nat Commun, 2019-07-18;10(1):3181.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. GSH-sensitive Pt(IV) prodrug-loaded phase-transitional nanoparticles with a hybrid lipid-polymer shell for precise theranostics against ovarian cancer
    Authors: H Huang, Y Dong, Y Zhang, D Ru, Z Wu, J Zhang, M Shen, Y Duan, Y Sun
    Theranostics, 2019-01-30;9(4):1047-1065.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Osteopontin mediates glioblastoma-associated macrophage infiltration and is a therapeutic target
    Authors: J Wei, A Marisetty, B Schrand, K Gabrusiewi, Y Hashimoto, M Ott, Z Grami, LY Kong, X Ling, HG Caruso, S Zhou, YA Wang, GN Fuller, JT Huse, E Gilboa, N Kang, X Huang, R Verhaak, S Li, AB Heimberger
    J. Clin. Invest., 2018-11-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. cRGD peptide installation on cisplatin-loaded nanomedicines enhances efficacy against locally advanced head and neck squamous cell carcinoma bearing cancer stem-like cells
    Authors: K Miyano, H Cabral, Y Miura, Y Matsumoto, Y Mochida, H Kinoh, K Iwata, O Nagano, H Saya, N Nishiyama, K Kataoka, T Yamasoba
    J Control Release, 2017-06-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Direct contact with perivascular tumor cells enhances integrin �v?3 signaling and migration of endothelial cells
    Authors: ME Burgett, JD Lathia, P Roth, AS Nowacki, DS Galileo, E Pugacheva, P Huang, A Vasanji, M Li, T Byzova, T Mikkelsen, S Bao, JN Rich, M Weller, CL Gladson
    Oncotarget, 2016-07-12;7(28):43852-43867.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice.
    Authors: Shen Y, Yang F, Wang H, Cai Z, Xu Y, Zhao A, Su Y, Zhang G, Zhu S
    PLoS ONE, 2016-01-22;11(1):e0147173.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Phagocytosis of apoptotic or necrotic cells differentially regulates the transcriptional expression of IL-12 family members in dendritic cells.
    Authors: Dixon K, O'Flynn J, van der Kooij S, van Kooten C
    J Leukoc Biol, 2014-04-29;96(2):313-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Involvement of angiopoietin-like 4 in matrix remodeling during chondrogenic differentiation of mesenchymal stem cells.
    Authors: Mathieu M, Iampietro M, Chuchana P, Guerit D, Djouad F, Noel D, Jorgensen C
    J Biol Chem, 2014-02-06;289(12):8402-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC, Neutralization
  12. Degradation of internalized alphaVbeta5 integrin is controlled by uPAR bound uPA: effect on beta1 integrin activity and alpha-SMA stress fiber assembly.
    Authors: Wang L, Pedroja BS, Meyers EE, Garcia AL, Twining SS, Bernstein AM
    PLoS ONE, 2012-03-21;7(3):e33915.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2010-10-07;134(2):167-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human Integrin alpha V beta 5 Antibody

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Human Integrin  alpha V beta 5 Antibody
By Adam Guess on 01/10/2017
Application: Flow Sample Tested: Mesenchymal stem cells Species: Human
Flow Cytometry Human Integrin  alpha V beta 5 Antibody MAB2528