Human Integrin alpha 4 beta 1 (Research Grade Natalizumab Biosimilar) Antibody

Catalog #: MAB10603 Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB10603-100
MAB10603-SP

Detection of Integrin alpha 4 beta 1 in Human PBMC by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin alpha 4 beta 1 (Research Grade Natalizumab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha 4 beta 1 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Natalizumab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₄ Clone # Hu114

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human Integrin alpha 4 beta 1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human PBMC

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha 4 beta 1 in Human PBMC by Flow Cytometry. Human PBMC were stained with (A) Human Anti-Human Integrin alpha 4 beta 1 (Research Grade Natalizumab Biosimilar) Monoclonal Antibody (Catalog # MAB10603) or (B) no primary antibody, followed by anti-Human IgG APC-conjugated Secondary Antibody (F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (FAB100P). Cells were stained using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4 beta 1

Natalizumab is a biosimilar directed against the extracellular domain of Human Integrin alpha 4 beta 1. It has been know to treat multiple sclerosis and Crohn's disease. Human Integrin alpha 4 beta 1 (Research Grade Natalizumab Biosimilar) Antibody is designd to be used in research use only.

Entrez Gene IDs

3676 (Human)

Alternate Names

CD49d; IA4; Integrin alpha 4 beta 1