Human IL-6R alpha Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 134 | 644 | 1669 | 130 | 645 | 1593 |
Standard Deviation | 11.5 | 16.8 | 38.3 | 8.3 | 26.9 | 75.4 |
CV% | 8.6 | 2.6 | 2.3 | 6.4 | 4.2 | 4.7 |
Recovery
The recovery of IL-6 sR spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 93 | 85-97 |
Citrate Plasma (n=4) | 93 | 86-102 |
EDTA Plasma (n=4) | 93 | 76-107 |
Heparin Plasma (n=4) | 101 | 87-118 |
Serum (n=4) | 93 | 86-110 |
Urine (n=4) | 86 | 64-98 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: IL-6R alpha
IL-6 R alpha is a transmembrane subunit of the receptor for Interleukin-6. It associates with gp130 which is a shared component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. IL-6 plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process twice for a total of 3 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 3 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human IL-6R alpha Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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IL-6 trans-signaling in a humanized mouse model of scleroderma
Authors: Odell, ID;Agrawal, K;Sefik, E;Odell, AV;Caves, E;Kirkiles-Smith, NC;Horsley, V;Hinchcliff, M;Pober, JS;Kluger, Y;Flavell, RA;
Proceedings of the National Academy of Sciences of the United States of America
Species: Human
Sample Types: Cell Culture Supernates
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Circulating Microvesicles in Association with the NLRP3 Inflammasome in Coronary Thrombi from STEMI Patients
Authors: V Bratseth, J Nordeng, R Helseth, S Solheim, S Åkra, H Arnesen, G Chiva-Blan, I Seljeflot
Biomedicines, 2022-09-05;10(9):.
Species: Human
Sample Types: Plasma
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Serum and Vascular Stiffness Biomarkers Associated with the Severity of Degenerative Aortic Valve Stenosis and Cardiovascular Outcomes
Authors: J Baran, ? Niewiara, J Podolec, M Siedli?ski, E Józefczuk, A Bernacik, R Badacz, T Przew?ocki, P Pieni??ek, K ?mudka, J Legutko, A Kab?ak-Zie
Oncogene, 2022-06-17;9(6):.
Species: Human
Sample Types: Serum
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Evaluating immune response and metabolic related biomarkers pre-allogenic hematopoietic stem cell transplant in acute myeloid leukemia
Authors: S Siamakpour, F Cao, J Lyu, Y Ren, AB Nixon, J Xie, AT Bush, MD Starr, JR Bain, MJ Muehlbauer, O Ilkayeva, V Byers Krau, JL Huebner, NJ Chao, AD Sung
PLoS ONE, 2022-06-14;17(6):e0268963.
Species: Human
Sample Types: Plasma
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Co-Occurrence of Interleukin-6 Receptor Asp358Ala Variant and High Plasma Levels of IL-6: An Evidence of IL-6 Trans-Signaling Activation in Deep Vein Thrombosis (DVT) Patients
Authors: R Salemi, G Gattuso, B Tomasello, A Lavoro, A Gaudio, M Libra, SS Signorelli, S Candido
Biomolecules, 2022-05-10;12(5):.
Species: Human
Sample Types: Plasma
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Dexmedetomidine attenuates oxygen-glucose deprivation/ reperfusion-induced inflammation through the miR-17-5p/ TLR4/ NF-kappaB axis
Authors: L Suo, M Wang
BMC anesthesiology, 2022-04-29;22(1):126.
Species: Rat
Sample Types:
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Cellular Response against Oxidative Stress, a Novel Insight into Lupus Nephritis Pathogenesis
Authors: CD Ene, SR Georgescu, M Tampa, C Matei, CI Mitran, MI Mitran, MN Penescu, I Nicolae
Journal of personalized medicine, 2021-07-22;11(8):.
Species: Human
Sample Types: Serum
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The Inflammasome Signaling Pathway Is Actively Regulated and Related to Myocardial Damage in Coronary Thrombi from Patients with STEMI
Authors: J Nordeng, H Schandiz, S Solheim, S Åkra, P Hoffman, B Roald, B Bendz, H Arnesen, R Helseth, I Seljeflot
Mediators of Inflammation, 2021-05-27;2021(0):5525917.
Species: Human
Sample Types: Serum
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Posttranslational Modifications Pattern in Clear Cell Renal Cell Carcinoma
Authors: CD Ene, MN Penescu, SR Georgescu, M Tampa, I Nicolae
Metabolites, 2020-12-27;11(1):.
Species: Human
Sample Types: Serum
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Inflammatory biomarkers, geriatric assessment, and treatment outcomes in acute myeloid leukemia
Authors: KP Loh, JA Tooze, BJ Nicklas, SB Kritchevsk, JD Williamson, LR Ellis, BL Powell, TS Pardee, NG Goyal, HD Klepin
J Geriatr Oncol, 2019-04-05;0(0):.
Species: Human
Sample Types: Plasma
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Management of Juvenile Idiopathic Arthritis in ABO-incompatible Kidney Transplantation: A Case Report
Authors: S Ishikawa, M Tasaki, T Kuroda, D Kobayashi, K Saito, Y Nakagawa, M Ikeda, K Takahashi, Y Tomita
Transplant. Proc., 2018-04-01;50(3):869-872.
Species: Human
Sample Types: Serum
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Adipogenic differentiation of MSC alters their immunomodulatory properties in a tissue-specific manner
Authors: H Munir, LS Ward, L Sheriff, S Kemble, S Nayar, F Barone, GB Nash, HM McGettrick
Stem Cells, 2017-04-24;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Inhibitory Mechanism of the Outer Membrane Growth of Chronic Subdural Hematomas
Authors: Koji Osuka
J. Neurotrauma, 2017-02-27;0(0):.
Species: Human
Sample Types: Chronic Subdural Hematoma Fluids
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Prevalent peripheral arterial disease and inflammatory burden
BMC Geriatr, 2016-12-09;16(1):213.
Species: Human
Sample Types: Serum
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IL-6 trans-signaling is another pathway to upregulate Osteopontin
Cytokine, 2016-11-15;90(0):88-95.
Species: Human
Sample Types: Cell Culture Supernates
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Inflammatory Markers and the Risk of Hip and Vertebral Fractures in Men: the Osteoporotic Fractures in Men (MrOS)
J Bone Miner Res, 2016-07-25;0(0):.
Species: Human
Sample Types: Serum
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Associations of total and free 25OHD and 1,25(OH)2D with serum markers of inflammation in older men.
Authors: P Srikanth, R F Chun, M Hewison, J S Adams, R Bouillon, D Vandersch, N Lane, P M Cawthon, T Dam, E Barrett-C, L B Daniels, J M Shikany, M L Stefanick, J A Cauley, E S Orwoll, C M Nielson
Osteoporosis International : a Journal Established as Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the Usa, 2016-02-23;0(0):1433-2965.
Species: Human
Sample Types: Serum
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A Novel IL6 Antibody Sensitizes Multiple Tumor Types to Chemotherapy Including Trastuzumab-Resistant Tumors.
Authors: Zhong H, Davis A, Ouzounova M, Carrasco R, Chen C, Breen S, Chang Y, Huang J, Liu Z, Yao Y, Hurt E, Moisan J, Fung M, Tice D, Clouthier S, Xiao Z, Wicha M, Korkaya H, Hollingsworth R
Cancer Res, 2016-01-07;76(2):480-90.
Species: Human
Sample Types: Cell Culture Supernates
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Stromal cells positively and negatively modulate the growth of cancer cells: stimulation via the PGE2-TNFalpha-IL-6 pathway and inhibition via secreted GAPDH-E-cadherin interaction.
Authors: Kawada M, Inoue H, Ohba S, Yoshida J, Masuda T, Yamasaki M, Usami I, Sakamoto S, Abe H, Watanabe T, Yamori T, Shibasaki M, Nomoto A
PLoS ONE, 2015-03-18;10(3):e0119415.
Species: Human
Sample Types: Cell Culture Supernates
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Blood stem cell fate regulation by Delta-1-mediated rewiring of IL-6 paracrine signaling.
Authors: Csaszar, Elizabet, Wang, Weijia, Usenko, Tatiana, Qiao, Wenlian, Delaney, Colleen, Bernstein, Irwin D, Zandstra, Peter W
Blood, 2013-11-15;123(5):650-8.
Species: Human
Sample Types: Cell Culture Supernates
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An autoinflammatory neurological disease due to interleukin 6 hypersecretion.
Authors: Salsano, Ettore, Rizzo, Ambra, Bedini, Gloria, Bernard, Loris, Dall'olio, Valentin, Volorio, Sara, Lazzaroni, Monica, Ceccherini, Isabella, Lazarevic, Dejan, Cittaro, Davide, Stupka, Elia, Paterra, Rosina, Farina, Laura, Savoiardo, Mario, Pareyson, Davide, Sciacca, Francesc
J Neuroinflammation, 2013-02-21;10(0):29.
Species: Human
Sample Types: Serum
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Bruton tyrosine kinase inhibition is a novel therapeutic strategy targeting tumor in the bone marrow microenvironment in multiple myeloma.
Authors: Tai Y, Chang B, Kong S, Fulciniti M, Yang G, Calle Y, Hu Y, Lin J, Zhao J, Cagnetta A, Cea M, Sellitto M, Zhong M, Wang Q, Acharya C, Carrasco D, Buggy J, Elias L, Treon S, Matsui W, Richardson P, Munshi N, Anderson K
Blood, 2012-06-11;120(9):1877-87.
Species: Human
Sample Types: Recombinant Protein
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Inflammatory markers and the risk of hip fracture: the Women's Health Initiative.
Authors: Barbour KE, Boudreau R, Danielson ME, Youk AO, Wactawski-Wende J, Greep NC, LaCroix AZ, Jackson RD, Wallace RB, Bauer DC, Allison MA, Cauley JA
J. Bone Miner. Res., 2012-05-01;27(5):1167-76.
Species: Human
Sample Types: Serum
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Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring.
Authors: Holmlund U, Amoudruz P, Johansson MA, Haileselassie Y, Ongoiba A, Kayentao K, Traore B, Doumbo S, Schollin J, Doumbo O, Montgomery SM, Sverremark-Ekstrom E
Clin. Exp. Immunol., 2010-10-05;162(3):500-9.
Species: Human
Sample Types: Milk
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Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
Transplantation, 2009-11-15;88(9):1109-16.
Species: Human
Sample Types: Urine
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Functional interaction of plasmacytoid dendritic cells with multiple myeloma cells: a therapeutic target.
Authors: Chauhan D, Singh AV, Brahmandam M, Carrasco R, Bandi M, Hideshima T, Bianchi G, Podar K, Tai YT, Mitsiades C, Raje N, Jaye DL, Kumar SK, Richardson P, Munshi N, Anderson KC
Cancer Cell, 2009-10-06;16(4):309-23.
Species: Human
Sample Types: Serum
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Daily family assistance and inflammation among adolescents from Latin American and European backgrounds.
Authors: Fuligni AJ, Telzer EH, Bower J, Irwin MR, Kiang L, Cole SW
Brain Behav. Immun., 2009-03-09;23(6):803-9.
Species: Human
Sample Types: Plasma
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Soluble and membrane levels of molecules involved in the interaction between clonal plasma cells and the immunological microenvironment in multiple myeloma and their association with the characteristics of the disease.
Authors: Perez-Andres M, Almeida J, Martin-Ayuso M, De Las Heras N, Moro MJ, Martin-Nunez G, Galende J, Cuello R, Abuin I, Moreno I, Dominguez M, Hernandez J, Mateo G, San Miguel JF, Orfao A
Int. J. Cancer, 2009-01-15;124(2):367-75.
Species: Human
Sample Types: Plasma
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Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
Authors: Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA
Cancer Res., 2009-01-01;69(1):329-37.
Species: Human
Sample Types: Serum
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Interleukin-6 and soluble interleukin-6 receptor levels as markers of disease extent and prognosis in neuroblastoma.
Authors: Egler RA, Burlingame SM, Nuchtern JG, Russell HV
Clin. Cancer Res., 2008-11-01;14(21):7028-34.
Species: Human
Sample Types: Plasma
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Improved prediction of disease relapse after radical prostatectomy through a panel of preoperative blood-based biomarkers.
Authors: Shariat SF, Karam JA, Walz J, Roehrborn CG, Montorsi F, Margulis V, Saad F, Slawin KM, Karakiewicz PI
Clin. Cancer Res., 2008-06-15;14(12):3785-91.
Species: Human
Sample Types: Plasma
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Inflammatory markers and incident fracture risk in older men and women: the Health Aging and Body Composition Study.
Authors: Cauley JA, Danielson ME, Boudreau RM, Forrest KY, Zmuda JM, Pahor M, Tylavsky FA, Cummings SR, Harris TB, Newman AB
J. Bone Miner. Res., 2007-07-01;22(7):1088-95.
Species: Human
Sample Types: Serum
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Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy.
Authors: Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G
AIDS Res. Hum. Retroviruses, 2007-07-01;23(7):886-90.
Species: Human
Sample Types: Plasma
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Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils.
Authors: Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J
Blood, 2007-06-13;110(6):1748-55.
Species: Human
Sample Types: Cell Culture Supernates
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Functional characterization of a soluble gp130 isoform and its therapeutic capacity in an experimental model of inflammatory arthritis.
Authors: Richards PJ, Nowell MA, Horiuchi S, McLoughlin RM, Fielding CA, Grau S, Yamamoto N, Ehrmann M, Rose-John S, Williams AS, Topley N, Jones SA
Arthritis Rheum., 2006-05-01;54(5):1662-72.
Species: Human
Sample Types: Serum
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Effect of influenza vaccine on markers of inflammation and lipid profile.
Authors: Tsai MY, Hanson NQ, Straka RJ, Hoke TR, Ordovas JM, Peacock JM, Arends VL, Arnett DK
J. Lab. Clin. Med., 2005-06-01;145(6):323-7.
Species: Human
Sample Types: Plasma
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Levels of soluble cytokine factors in temporomandibular joint effusions seen on magnetic resonance images.
Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005-04-01;99(4):411-8.
Species: Human
Sample Types: Synovial Fluid
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Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
Transpl. Int., 2005-02-01;18(2):177-85.
Species: Human
Sample Types: Plasma
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Circulating interleukin-6 family cytokines and their receptors in patients with congestive heart failure.
Authors: Hirota H, Izumi M, Hamaguchi T, Sugiyama S, Murakami E, Kunisada K, Fujio Y, Oshima Y, Nakaoka Y, Yamauchi-Takihara K
Heart Vessels, 2004-09-01;19(5):237-41.
Species: Human
Sample Types: Plasma
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Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
J. Virol., 2003-02-01;77(3):1727-37.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Serum
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Effects of IL-6 variants in multiple myeloma: growth inhibition and induction of apoptosis in primary cells.
Authors: Petrucci MT, Ricciardi MR, Gregorj C, Ciapponi L, Savino R, Ciliberto G, Tafuri A
Leuk. Lymphoma, 2002-12-01;43(12):2369-75.
Species: Human
Sample Types: Serum
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Interleukin-6 is a major effector molecule of short-term G-CSF treatment inducing bone metabolism and an acute-phase response.
Authors: Carstanjen D, Regenfus M, Muller C, Salama A
Exp. Hematol., 2001-07-01;29(7):812-21.
Species: Human
Sample Types: Plasma
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Ovariectomy does not induce osteopenia through interleukin-6 in rhesus monkeys (Macaca mulatta).
Authors: Keller ET, 49536, Binkley NC, Stebler BA, Hall DM, Johnston GM, Zhang J, Ershler WB
CpG Type A Induction of an Early Protective Environment in Experimental Multiple Sclerosis, 2000-01-01;26(1):55-62.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Bone Marrow Fluid
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Discriminant power of combined cerebrospinal fluid tau protein and of the soluble interleukin-6 receptor complex in the diagnosis of Alzheimer's disease.
Authors: Hampel H, Teipel SJ, Padberg F, Haslinger A, Riemenschneider M, Schwarz MJ, Kotter HU, Scheloske M, Buch K, Stubner S, Dukoff R, Lasser R, Moller N, Sunderland T, Rapoport SI, Moller HJ
Brain Res., 1999-03-27;823(1):104-12.
Species: Human
Sample Types: CSF
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Interleukin-6 and the soluble IL-6 receptor are decreased in cerebrospinal fluid of geriatric patients with major depression: no alteration of soluble gp130.
Authors: Stubner S, Schon T, Padberg F, Teipel SJ, Schwarz MJ, Haslinger A, Buch K, Dukoff R, Lasser R, Moller N, Sunderland T, Rapoport SI, Moller HJ, Hampel H
Neurosci. Lett., 1999-01-15;259(3):145-8.
Species: Human
Sample Types: CSF
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