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Human IL-6R alpha Antibody

Catalog #: MAB227
32 Citations 5 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB227-SP
MAB227-100
MAB227-500
Detection of Recombinant Human IL-6R alpha by Western Blot.
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Product Details
Citations (32)
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Supplemental Products
Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-6R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-6R alpha in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) IL-1 alpha, recombinant mouse (rm) IL-1 alpha, rhIL-1 beta, rmIL-1 beta, rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.
Source
Monoclonal Mouse IgG1 Clone # 17506
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha
Leu20-Asp339
Accession # P08887
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human IL-6R alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IL-6R alpha Biotinylated Antibody (Catalog # BAF227)

Standard: Recombinant Human IL-6R alpha Protein (Catalog # 227-SR)

Neutralization

Measured by its ability to neutralize IL-6R alpha-mediated enhancement of IL‑6-dependent Inhibition of cell proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 0.04-0.2 µg/mL in the presence of 30 ng/mL Recombinant Human IL-6R alpha and 30 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human IL-6R alpha antibody by Western Blot. View Larger

Detection of Recombinant Human IL-6R alpha by Western Blot. Western blot shows 25 ng of Recombinant Human IL-6R alpha (Catalog # 227-SR) and Recombinant Mouse IL-6R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Flow Cytometry Detection of IL-6R alpha antibody in U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IL-6R alpha in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was labeled with Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Neutralization IL-6R alpha Enhancement of IL‑6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6R alpha Antibody. View Larger

IL-6R alpha Enhancement of IL‑6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6R alpha Antibody. Recombinant Human IL-6R alpha (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant HumanIL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227). The ND50 is typically 0.04-0.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6R alpha

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

References
  1. Yamasaki et al. (1988) Science 241:825.
  2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
  3. Hibi et al. (1990) Cell 63:1149.
  4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
  5. Novick et al. (1989) J. Exp. Med. 170:1409.
  6. Yasukawa et al. (1990) J. Biochem. 108:673.
  7. Saito et al. (1991) J. Immunology 147:168.
Long Name
Interleukin 6 Receptor alpha
Entrez Gene IDs
3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names
CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor

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Citations for Human IL-6R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. A functional role for the cancer disparity-linked genes, CRY beta B2 and CRY beta B2P1, in the promotion of breast cancer
    Authors: Barrow MA, Martin ME, Coffey A et al.
    Breast Cancer Res.
  2. Uncovering mutation-specific morphogenic phenotypes and paracrine-mediated vessel dysfunction in a biomimetic vascularized mammary duct platform
    Authors: ML Kutys, WJ Polacheck, MK Welch, KA Gagnon, T Koorman, S Kim, L Li, AI McClatchey, CS Chen
    Nat Commun, 2020-07-06;11(1):3377.
  3. Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis.
    Authors: Komori M, Blake A, Greenwood M et al.
    Ann Neurol
  4. Stromal heterogeneity may explain increased incidence of metaplastic breast cancer in women of African descent
    Authors: Kumar, B;Khatpe, AS;Guanglong, J;Batic, K;Bhat-Nakshatri, P;Granatir, MM;Addison, RJ;Szymanski, M;Baldridge, LA;Temm, CJ;Sandusky, G;Althouse, SK;Cote, ML;Miller, KD;Storniolo, AM;Nakshatri, H;
    Nature communications
  5. Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis
    Authors: S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood
    Nature Communications, 2022-06-03;13(1):3121.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma
    Authors: Karavyraki M, Porter RK.
    Medical Oncology
  7. Molecular signaling pathways in right ventricular impairment of adult patients after tetralogy of Fallot repair
    Authors: Kathrin Pollmann, Sarala Raj Murthi, Damir Kračun, Thomas Schwarzmayr, Andreas Petry, Julie Cleuziou et al.
    Cardiovascular Diagnosis and Therapy
  8. TM4SF5-dependent crosstalk between hepatocytes and macrophages to reprogram the inflammatory environment
    Authors: E Kim, H Um, J Park, JW Jung, JE Kim, H Lee, EA Shin, Y Pinanga, H Lee, SH Nam, JW Lee
    Cell Reports, 2021-11-16;37(7):110018.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Peficitinib improves bone fragility by recovering bone turnover imbalance in arthritic mice
    Authors: S Sugahara, K Hanaoka, T Emori, N Takeshita, Y Fujii, M Nakano, T Suzuki, J Takahashi, Y Nakamura
    Journal of pharmacological sciences, 2021-11-03;148(1):134-141.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Interleukin-6 released from differentiating human beige adipocytes improves browning
    Authors: Endre Kristóf, Ágnes Klusóczki, Roland Veress, Abhirup Shaw, Zsolt Sándor Combi, Klára Varga et al.
    Experimental Cell Research
  11. The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by ?-PD-L1 or ?-IL6 antibodies
    Authors: C Rolvering, AD Zimmer, A Ginolhac, C Margue, M Kirchmeyer, F Servais, HM Hermanns, S Hergovits, PV Nazarov, N Nicot, S Kreis, S Haan, I Behrmann, C Haan
    J. Leukoc. Biol., 2018-07-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. IL-6 Trans–Signaling Links Inflammation with Angiogenesis in the Peritoneal Membrane
    Authors: Rusan Catar, Janusz Witowski, Nan Zhu, Christian Lücht, Alicia Derrac Soria, Javier Uceda Fernandez et al.
    Journal of the American Society of Nephrology
  13. IL-6 trans-signaling is another pathway to upregulate Osteopontin
    Cytokine, 2016-11-15;90(0):88-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium
    PLoS ONE, 2016-05-12;11(5):e0155161.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. IL6 secreted by Ewing sarcoma tumor microenvironment confers anti-apoptotic and cell-disseminating paracrine responses in Ewing sarcoma cells
    Authors: Andrej Lissat, Mandy Joerschke, Dheeraj A. Shinde, Till Braunschweig, Angelina Meier, Anna Makowska et al.
    BMC Cancer
  16. Sphingosine 1-phosphate has anti-apoptotic effect on liver sinusoidal endothelial cells and proliferative effect on hepatocytes in a paracrine manner in human
    Authors: Takeshi Nowatari, Soichiro Murata, Ken Nakayama, Naoki Sano, Takehito Maruyama, Reiji Nozaki et al.
    Hepatology Research
  17. Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
    Authors: Sommer A, Fabri M
    PLoS ONE, 2015-06-24;10(6):e0130395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Fibroblasts Influence Survival and Therapeutic Response in a 3D Co-Culture Model
    Authors: Meher Majety, Leon P. Pradel, Manuela Gies, Carola H. Ries
    PLOS ONE
  19. Contact-dependent depletion of hydrogen peroxide by catalase is a novel mechanism of myeloid-derived suppressor cell induction operating in human hepatic stellate cells.
    Authors: Resheq Y, Li K, Ward S, Wilhelm A, Garg A, Curbishley S, Blahova M, Zimmermann H, Jitschin R, Mougiakakos D, Mackensen A, Weston C, Adams D
    J Immunol, 2015-02-09;194(6):2578-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Mutant B-RAF-Mcl-1 survival signaling depends on the STAT3 transcription factor.
    Authors: Becker, T M, Boyd, S C, Mijatov, B, Gowrishankar, K, Snoyman, S, Pupo, G M, Scolyer, R A, Mann, G J, Kefford, R F, Zhang, X D, Rizos, H
    Oncogene, 2013-03-04;33(9):1158-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta.
    Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
    Nature, 2012-04-26;484(7395):514-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
    Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E
    Nat. Med., 2011-10-30;17(11):1498-503.
    Species: Human
    Sample Types: In Vivo
    Applications: In Vivo
  23. Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells.
    Authors: Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2009-07-05;10(8):857-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
    Authors: Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA
    Cancer Res., 2009-01-01;69(1):329-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  25. IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.
    Authors: Kolliputi N, Waxman AB
    Am. J. Respir. Cell Mol. Biol., 2008-09-05;40(3):314-24.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
    Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007-08-05;8(9):942-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle.
    Authors: Ammit AJ, Moir LM, Oliver BG, Hughes JM, Alkhouri H, Ge Q, Burgess JK, Black JL, Roth M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006-08-25;292(1):L199-206.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  29. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002-11-01;169(9):5244-51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  30. Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells.
    Authors: Erices A, Conget P, Rojas C, Minguell JJ
    Exp. Cell Res., 2002-10-15;280(1):24-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  31. Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
    Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
    Immunity, 2001-06-01;14(6):705-14.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ELISA Development, Flow Cytometry
  32. A Magnetic Bead-Based Sensor for the Quantification of Multiple Prostate Cancer Biomarkers.
    Authors: Jokerst JV, Chen Z, Xu L et al.
    PLoS One

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Human IL-6R alpha Antibody
By Anonymous on 04/03/2023
Application: WB Sample Tested: Spleen tissue Species: Human

Western blot was performed on human spleen tissue using 1 µg/mL of Human IL-6R alpha Antibody.

Western Blot Human IL-6R alpha Antibody MAB227
Human IL-6R alpha Antibody
By Katie Myers on 11/28/2022
Application: ELISA Sample Tested: Serum Species: Primate

Worked as an ELISA capture antibody

ELISA Human IL-6R alpha Antibody MAB227
Human IL-6 R alpha Antibody
By Anonymous on 10/17/2018
Application: WB Sample Tested: HepG2 human hepatocellular carcinoma cell line,Huh-7 human hepatoma cell line Species: Human
Western Blot Human IL-6 R alpha Antibody MAB227
Human IL-6 R alpha Antibody
By Anonymous on 05/31/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Paired with BAF227 this antibody was used to measure IL-6 receptor in human plasma samples using a sandwich immunoassay.

ELISA Human IL-6 R alpha Antibody MAB227
Human IL-6 R alpha Antibody
By Anonymous on 11/29/2017
Application: WB Sample Tested: Stimulated Human Bronchial Epithelial Cell Line (BEAS-2B) Species: Human