Human IL-31 Antibody

Name: Human IL-31 Antibody
Brand: R&D Systems
SKU: MAB2824
Rating: 3 (1 reviews)

Catalog #: MAB2824 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2824
MAB2824-SP

All IL-31 Antibodies

Product Details

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-31 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-31 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) IL-31 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 308227

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IL-31
Ser24-Thr164
Accession # Q6EBC2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑31 (Catalog # 2824-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-31

Human Interleukin-31 (IL-31) is a 24 kDa, short-chain member of the alpha -helical family of cytokines. The human IL-31 cDNA encodes a 164 amino acid (aa) precursor that contains a 23 aa signal peptide and a 141 aa mature protein (1, 2). The mature region shows four alpha -helices which would be expected to show a typical up-up-down-down topology. Human and mouse IL-31 share 24% aa sequence identity in the mature region (1). IL-31 is mainly associated with activated T cells and preferentially expressed by Th2 rather than Th1 cells. IL-31 signals via a heterodimeric receptor complex composed of a 120 kDa, gp130-related molecule termed IL-31 RA (also GPL and GLM-R) and the 180 kDa oncostatin M receptor (OSM R beta ) (2‑6). In the complex, IL-31 directly binds to GPL, not OSM R (2, 3). IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and the MAP kinase pathway (2‑5). Although multiple isoforms of IL-31 RA are known, only a form that contains the entire length of the cytoplasmic domain is signaling-capable (2, 3). The IL-31 receptor is constitutively expressed by keratinocytes and upregulated by IFN-gamma on monocytes (1). Studies using transgenic mice indicate that IL-31 may contribute to the pruritis (itching) associated with nonatopic dermatitis (1, 7).

References