Human IL-27 R alpha /WSX-1/TCCR Antibody

Catalog #: AF1479
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1479
AF1479-SP

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Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-27 R alpha /WSX-1/TCCR Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-27 R alpha /WSX‑1/TCCR in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant mouse TCCR is observed.

Source

Polyclonal Goat IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL-27 R alpha /WSX‑1/TCCR Fc Chimera (Catalog # 1479-TC)

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human lymph node

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-27 R alpha/WSX-1/TCCR

IL‑27 R alpha (also known as WSX‑1 and TCCR) is a 96‑100 kDa member of the type I, group 2 cytokine receptor family (1, 2, 3, 4, 5, 6). Mature IL‑27 R alpha is a type I transmembrane glycoprotein that contains a 484 amino acid (aa) extracellular region, a 21 aa transmembrane segment and a 99 aa cytoplasmic domain. Consistent with type I cytokine receptors, the extracellular region contains four positionally conserved cysteine residues, a WSxWS motif (for receptor folding and ligand binding), and three fibronectin type III repeats. The intracellular domain contains a "box‑1" motif that may be involved with Janus kinases (3). One potential alternate splice form has been hypothesized that involves a 58 aa addition to the cytoplasmic domain and, based on mouse, a soluble 33 kDa splice form that shows a 20 aa substitution for aa 257‑636 may also occur in human (3, 7). The human IL‑27 R alpha extracellular region shares 63% amino acid identity with the mouse IL‑27 R alpha extracellular domain (2, 3). IL‑27 R alpha is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and naïve T cells (1, 2, 4, 8). Typical of other class I cytokine receptor chains, the ligand binding IL‑27 R alpha molecule is known to heterodimerize with a signal‑transducing subunit (gp130) to form a functional IL‑27 receptor (9, 10). In addition, IL‑27 R alpha is reported to complex with CNTFR alpha and gp130 form a humanin receptor on neurons (7, 11), and to complex with gp130 and IL‑6 R to form a receptor for a p28:CLF heterodimeric cytokine on lymphocytes (12). Studies using IL‑27 R alpha /WSX‑1^‑/‑ mice reveal that IL‑27 has the ability to suppress T cell activity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity (4, 13, 14). In particular, IL‑27 is known to act on naïve T cells, blocking their differentiation into a Th17 phenotype. Notably, cells committed to a Th17 phenotype, although they express a functional IL‑27 receptor, are unresponsive to the effects of IL‑27 (15). Activated T cells that are CD4⁺ and CD8⁺, and which express the IL‑27 receptor, can be induced by
IL‑27 to form a double‑positive CD25⁺ FoxP3^‑ IFN‑ gamma plus IL‑10 secreting phenotype that both promotes and suppresses the inflammatory response (16).

References

Villarino, A.V. et al. (2004) J. Immunol. 173:715.
Chen, Q. et al. (2000) Nature 407:916.
Sprecher, C.A. et al. (1998) Biochem. Biophys. Res. Commun. 246:82.
Artis, D. et al. (2004) J. Immunol. 173:5626.
Yoshida, H. & Y. Miyazaki (2008) Int. J. Biochem. Cell Biol. 40:2379.
Yoshida, H. & M. Yoshiyuki (2008) Immunol. Rev. 226:234.
Hashimoto, Y. et al. (2009) Biochem. Biophys. Res. Commun. 389:95.
Holscher, C. et al. (2005) J. Immunol. 174:3534.
Pflanz, S. et al. (2004) J. Immunol. 172:2225.
Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
Hashimoto, Y. et al. (2009) Mol. Biol. Cell 20:2864.
Crabe, S. et al. (2009) J. Immunol. 183:7692.
Villarino, A. et al. (2003) J. Immunol. 170:645.
Hamano., S. et al. (2003) Immunity 19:657.
El-behi, M. et al. (2009) J. Immunol. 183:4957.
Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.

Long Name

Interleukin-27 Receptor Subunit alpha

Entrez Gene IDs

9466 (Human); 50931 (Mouse)

Alternate Names

class I cytokine receptor; CRL1IL-27R; Cytokine receptor-like 1; IL-27 R alpha; IL27R alpha; IL27R; IL27RA; IL-27Ra; IL-27R-alpha; interleukin 27 receptor, alpha; interleukin-27 receptor subunit alpha; TCCR; TCCRIL-27 receptor subunit alpha; T-cell cytokine receptor type 1; Type I T-cell cytokine receptor; WSX-1; WSX1IL-27R subunit alpha; zcytor1

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Citations for Human IL-27 R alpha /WSX-1/TCCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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A soluble form of IL-27Ralpha is a natural IL-27 antagonist.
Authors: Dietrich C, Candon S, Ruemmele F, Devergne O
J Immunol, 2014-04-25;192(11):5382-9.
Species: Primate - Chlorocebus aethiops (African Green Monkey)
Sample Types: Cell Lysates
Applications: Immunoprecipitation
IL-27 abrogates receptor activator of NF-kappa B ligand-mediated osteoclastogenesis of human granulocyte-macrophage colony-forming unit cells through STAT1-dependent inhibition of c-Fos.
Authors: Furukawa M, Takaishi H, Takito J, Yoda M, Sakai S, Hikata T, Hakozaki A, Uchikawa S, Matsumoto M, Chiba K, Kimura T, Okada Y, Matsuo K, Yoshida H, Toyama Y
J. Immunol., 2009-07-20;183(4):2397-406.
Species: Human
Sample Types: Whole Cells
Applications: ICC