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Human IL-17/IL-17A Antibody

Catalog #: AF-317-NA
94 Citations 5 Reviews Datasheet / COA / SDS
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AF-317-NA
AF-317-SP
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Detection of Human IL‑17 by Western Blot.
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Citations (94)
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Human IL-17/IL-17A Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant canine IL-17 is observed.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below
Immunoprecipitation
3 µg/100 µg cell lysate
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
Immersion fixed human peripheral blood mononuclear cells treated with PHA
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below
Neutralization
Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.02-0.12 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-17 antibody by Western Blot. View Larger

Detection of Human IL‑17 by Western Blot. Western blot shows lysates of human primary naïve CD4+ T cells, along with whole cell lysates (WCL) and conditioned-media supernatant (Supe) of human primary differentiated Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-17 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry IL-17/IL-17A antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

IL‑17/IL‑17A in Human Tonsil. IL-17/IL-17A was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-17/IL-17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunoprecipitation Immunoprecipitation of Human IL‑17. View Larger

Immunoprecipitation of Human IL‑17. Human IL-17 was immunoprecipitated from 100 µg of human primary differentiated Th17 cell lysate following incubation with 3 µg Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) or control antibody (AB-108-C) overnight at 4 °C. IL-17-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

Intracellular Staining by Flow Cytometry Detection of IL-17 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑17 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca 2+ionomycin for 16 hours, then stained with Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA, dark orange filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Neutralization IL-6 Secretion Induced by IL-17 and Neutralization by Human IL-17 Antibody. View Larger

IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody. Recombinant Human IL-17 (317-ILB) stimulates IL-6 secretion in NIH/3T3 mouse embryonic fibroblasts in a dose-dependent manner (orange line), as measured by the mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Human IL-17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). The ND50 is typically 0.02‑0.12 µg/mL.

Immunohistochemistry Detection of Human IL-17/IL-17A by Immunohistochemistry View Larger

Detection of Human IL-17/IL-17A by Immunohistochemistry Expression of psoriasis‐related cytokines in paradoxical psoriasiform reactions. Immunohistochemistry analysis performed on paradoxical skin lesions obtained from patients 1 (Pt1) and 2 (Pt2) shows similar values of IL‐17A+ cells, a reduction of dermal IFN‐ gamma + cells and an increase of IL‐22+ or IL‐36 gamma + cells, when compared with psoriatic skin lesions. LS and NLS skin of the same psoriatic patient (n = 3) was analyzed. Graphs show the mean of number of positive cells + SD per three sections. One out of three representative stainings is shown. *p < 0.01, **p < 0.05, versus classical psoriasis. Scale bars, 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31577850), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human IL-17/IL-17A by Immunohistochemistry View Larger

Detection of Human IL-17/IL-17A by Immunohistochemistry Inflammatory cell infiltrates of early acne lesions in immunohistochemistry.Representative immunohistochemical staining showing IL-17A+ cells in early acne lesion (a). Staining of IL-17A+ and CD3+ cells shows that IL-17A (in green) and CD3 (in red) were detected in acne vulgaris lesions. Cell nuclei were counterstained in blue. Arrows indicate IL-17A and CD3 double positive cells (b). T-bet+ cells (c) were more numerous than IL-17A+ cells or Foxp3+ cells (d) in the lesional acne skin. Large number of CD4+ cells (e), mostly lymphocytes, was seen around pilosebaceous unit and perivascularly. CD8+ cells (f) were fewer in number than CD4+ cells. Large number of CD68+ macrophages (g) and a few CD83+ cells (h), which are mature dendritic cells, were detected around sebaceous follicles. (Bar = 200 µm; in the insets bar  = 50 µm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25153527), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human IL-17/IL-17A by Immunohistochemistry View Larger

Detection of Human IL-17/IL-17A by Immunohistochemistry Inflammatory cell infiltrates of early acne lesions in immunohistochemistry.Representative immunohistochemical staining showing IL-17A+ cells in early acne lesion (a). Staining of IL-17A+ and CD3+ cells shows that IL-17A (in green) and CD3 (in red) were detected in acne vulgaris lesions. Cell nuclei were counterstained in blue. Arrows indicate IL-17A and CD3 double positive cells (b). T-bet+ cells (c) were more numerous than IL-17A+ cells or Foxp3+ cells (d) in the lesional acne skin. Large number of CD4+ cells (e), mostly lymphocytes, was seen around pilosebaceous unit and perivascularly. CD8+ cells (f) were fewer in number than CD4+ cells. Large number of CD68+ macrophages (g) and a few CD83+ cells (h), which are mature dendritic cells, were detected around sebaceous follicles. (Bar = 200 µm; in the insets bar  = 50 µm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25153527), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-17/IL-17A

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has recently been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation.

Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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Citations for Human IL-17/IL-17A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

94 Citations: Showing 1 - 10
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  1. Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance
    Authors: Zheng N, Fang J, Xue G et al.
    Cancer cell
  2. A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa.
    Authors: Frew John W, Hawkes Jason E, Krueger James G
    F1000Research
  3. Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis
    Authors: Mick J.M. van Eijs, José J.M. Ter Linde, Matthijs J.D. Baars, Mojtaba Amini, Miangela M. Laclé, Eelco C. Brand et al.
    iScience
  4. Insight into immune profile associated with vitiligo onset and anti-tumoral response in melanoma patients receiving anti-PD-1 immunotherapy
    Authors: Maria Luigia Carbone, Alessia Capone, Marika Guercio, Sofia Reddel, Domenico Alessandro Silvestris, Daniela Lulli et al.
    Frontiers in Immunology
  5. Multilevel human secondary lymphoid immune system compartmentalization revealed by complementary imaging approaches
    Authors: Oyler BL, Valencia-Dávila JA, Moysi E et al.
    iScience
  6. IL17A mRNA Staining Distinguishes Palmoplantar Psoriasis from Hyperkeratotic Palmoplantar Eczema in Diagnostic Skin Biopsies
    Authors: Jennifer S. Chen, Michael J. Murphy, Katelyn Singh, Alice Wang, Ryan D. Chow, Sa Rang Kim et al.
    JID Innovations
  7. Clinical significance of CD155 expression and correlation with cellular components of tumor microenvironment in gastric adenocarcinoma
    Authors: Xue Liu, Chenyang Xu, Tianwei Guo, Shenghua Zhan, Qiuying Quan, Mengsi Li et al.
    Frontiers in Immunology
  8. Canakinumab leads to rapid reduction of neutrophilic inflammation and long-lasting response in Schnitzler syndrome
    Authors: Simon Bossart, S. Morteza Seyed Jafari, Kristine Heidemeyer, Kexiang Yan, Laurence Feldmeyer, Luca Borradori et al.
    Frontiers in Medicine
  9. Spatial transcriptomics landscape of lesions from non-communicable inflammatory skin diseases
    Authors: A Schäbitz, C Hillig, M Mubarak, M Jargosch, A Farnoud, E Scala, N Kurzen, AC Pilz, N Bhalla, J Thomas, M Stahle, T Biedermann, CB Schmidt-We, F Theis, N Garzorz-St, K Eyerich, MP Menden, S Eyerich
    Nature Communications, 2022-12-13;13(1):7729.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. The immune cell atlas of human neuroblastoma
    Authors: Bronte Manouk Verhoeven, Shenglin Mei, Thale Kristin Olsen, Karin Gustafsson, Anders Valind, Axel Lindström et al.
    Cell Reports Medicine
  11. MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data
    Authors: Daniëlle Krijgsman, Neeraj Sinha, Matthijs J.D. Baars, Stephanie van Dam, Mojtaba Amini, Yvonne Vercoulen
    STAR Protocols
  12. Single cell transcriptomic landscape of diabetic foot ulcers
    Authors: G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin
    Nature Communications, 2022-01-10;13(1):181.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. Reduced Interleukin-17-Expressing Cells in Cutaneous Melanoma
    Authors: A Tosi, L Nardinocch, ML Carbone, L Capriotti, E Pagani, S Mastroeni, C Fortes, F Scopelliti, C Cattani, F Passarelli, A Rosato, S D'Atri, CM Failla, A Cavani
    Biomedicines, 2021-12-16;9(12):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. Condomless receptive anal intercourse is associated with markers of mucosal inflammation in a cohort of men who have sex with men in Atlanta, Georgia
    Authors: CF Kelley, I Pollack, R Yacoub, Z Zhu, VE Van Doren, S Gumber, RR Amara, V Fedirko, CS Kraft, TJB de Man, YJ Hu, C Grimsley A, PS Sullivan, RM Bostick
    Journal of the International AIDS Society, 2021-12-01;24(12):e25859.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. IFN‐ gamma facilitates liver fibrogenesis by CD161+CD4+ T cells through a regenerative IL‐23/IL‐17 axis in chronic hepatitis B virus infection
    Authors: Jing Li, Lisha Cheng, Haoyu Jia, Chun Liu, Siqi Wang, Yun Liu et al.
    Clinical & Translational Immunology
  16. Chronic Hepatitis C Pathogenesis: Immune Response in the Liver Microenvironment and Peripheral Compartment
    Authors: Daniela Alejandra Rios, Paola Cecilia Casciato, María Soledad Caldirola, María Isabel Gaillard, Cecilia Giadans, Beatriz Ameigeiras et al.
    Frontiers in Cellular and Infection Microbiology
  17. ROR&gammat inhibitors block both IL-17 and IL-22 conferring a potential advantage over anti-IL-17 alone to treat severe asthma
    Authors: D Lamb, D De Sousa, K Quast, K Fundel-Cle, JS Erjefält, C Sandén, HJ Hoffmann, M Kästle, R Schmid, K Menden, D Delic
    Respiratory Research, 2021-05-22;22(1):158.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  18. Hepatic lymphocytes involved in the pathogenesis of pediatric and adult non-alcoholic fatty liver disease
    Authors: V Cairoli, E De Matteo, D Rios, C Lezama, M Galoppo, P Casciato, E Mullen, C Giadans, G Bertot, MV Preciado, P Valva
    Scientific Reports, 2021-03-04;11(1):5129.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  19. Interleukin-17A derived from mast cells contributes to fibrosis in gastric cancer with peritoneal dissemination
    Authors: Katsuya Gunjigake, Jun Kinoshita, Takahisa Yamaguchi, Hiroto Saito, Daisuke Fujimori, Toshihide Horiike et al.
    Gastric Cancer
  20. CD4 T cell-intrinsic role for the T helper 17 signature cytokine IL-17: Effector resistance to immune suppression
    Authors: Michael P. Crawford, Sushmita Sinha, Pranav S. Renavikar, Nicholas Borcherding, Nitin J. Karandikar
    Proceedings of the National Academy of Sciences
  21. Safe and Successful Treatment of Acute Cellular Rejection of an Intestine and Abdominal Wall Transplant With Vedolizumab
    Authors: Guido Trentadue, Gursah Kats-Ugurlu, Tjasso Blokzijl, Gilles FH Diercks, Jan Willem Haveman, Klaas Nico Faber et al.
    Transplantation Direct
  22. Ex vivo culture of lesional psoriasis skin for pharmacological testing
    Authors: ML Tiirikaine, A Woetmann, H Norsgaard, LF Santamaria, P Lovato
    J. Dermatol. Sci., 2019-12-27;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Tissue Culture
  23. Paradoxical psoriasis induced by Tnf-alpha blockade shows immunological features typical of the early phase of psoriasis development
    Authors: L Fania, M Morelli, C Scarponi, L Mercurio, F Scopelliti, C Cattani, GL Scaglione, T Tonanzi, MA Pilla, G Pagnanelli, C Mazzanti, G Girolomoni, A Cavani, S Madonna, C Albanesi
    J Pathol Clin Res, 2019-10-29;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  24. Interleukin (IL)-13 and IL-17A contribute to neo-osteogenesis in chronic rhinosinusitis by inducing RUNX2
    Authors: R Khalmurato, HW Shin, DW Kim, JW Park
    EBioMedicine, 2019-07-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  25. Interleukin-17 Promotes Migration and Invasion of Human Cancer Cells Through Upregulation of MTA1 Expression
    Authors: Na Guo, Ge Shen, Ying Zhang, Ahmed A. Moustafa, Dongxia Ge, Zongbing You
    Frontiers in Oncology
  26. A systematic review and critical evaluation of inflammatory cytokine associations in hidradenitis suppurativa
    Authors: John W. Frew, Jason E. Hawkes, James G. Krueger
    F1000Research
  27. Composition and Clinical Impact of the Immunologic Tumor Microenvironment in Oral Squamous Cell Carcinoma
    Authors: M Boxberg, L Leising, K Steiger, M Jesinghaus, A Alkhamas, M Mielke, N Pfarr, C Götz, KD Wolff, W Weichert, A Kolk
    J. Immunol., 2018-12-10;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Lutzomyia longipalpis Saliva Drives Interleukin-17-Induced Neutrophil Recruitment Favoring Leishmania infantum Infection
    Authors: Clarissa R. Teixeira, Claire da S. Santos, Deboraci B. Prates, Rafael T. dos Santos, Théo Araújo-Santos, Sebastião M. de Souza-Neto et al.
    Frontiers in Microbiology
  29. A Reappraisal on the Potential Ability of Human Neutrophils to Express and Produce IL-17 Family Members In Vitro: Failure to Reproducibly Detect It
    Authors: Nicola Tamassia, Fabio Arruda-Silva, Federica Calzetti, Silvia Lonardi, Sara Gasperini, Elisa Gardiman et al.
    Frontiers in Immunology
  30. Increased interleukin-17A levels promote rituximab resistance by suppressing p53 expression and predict an unfavorable prognosis in patients with diffuse large B cell lymphoma
    Authors: Weijie Zhong, Xin Xu, Zhigang Zhu, Li Yang, Hong Du, Zhongjun Xia et al.
    International Journal of Oncology
  31. Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis
    Authors: DA Rios, P Valva, PC Casciato, S Frias, M Soledad Ca, MI Gaillard, L Bezrodnik, J Bandi, O Galdame, B Ameigeiras, D Krasniansk, C Brodersen, E Mullen, EN Matteo, MV Preciado
    Sci Rep, 2017-10-16;7(1):13283.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  32. Zoonotic intestinal helminths interact with the canine immune system by modulating T cell responses and preventing dendritic cell maturation
    Authors: J Junginger, K Raue, K Wolf, E Janecek, VM Stein, A Tipold, AR Günzel-Ape, C Strube, M Hewicker-T
    Sci Rep, 2017-09-04;7(1):10310.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Expression of IL-23/Th17-related cytokines in basal cell carcinoma and in the response to medical treatments
    Authors: C Pellegrini, A Orlandi, G Costanza, A Di Stefani, A Piccioni, A Di Cesare, A Chiricozzi, A Ferlosio, K Peris, MC Fargnoli
    PLoS ONE, 2017-08-22;12(8):e0183415.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  34. Idiopathic subglottic stenosis is associated with activation of the inflammatory IL-17A/IL-23 axis
    Authors: Alexander Gelbard, Nicolas-George Katsantonis, Masanobu Mizuta, Dawn Newcomb, Joseph Rotsinger, Bernard Rousseau et al.
    The Laryngoscope
  35. Characterization of IL-17AA and IL-17FF in rheumatoid arthritis and multiple sclerosis
    Authors: Cheryl Schofield, Saloumeh K Fischer, Michael J Townsend, Sofia Mosesova, Kun Peng, Alvernia Francesca Setiadi et al.
    Bioanalysis
  36. Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells
    Authors: CT Seebauer, S Brunner, G Glockzin, P Piso, P Ruemmele, HJ Schlitt, EK Geissler, S Fichtner-F, R Kesselring
    Oncoimmunology, 2016-10-14;5(12):e1242543.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  37. Atopy and Inhaled Corticosteroid Use Associate with Fewer IL-17+ Cells in Asthmatic Airways
    PLoS ONE, 2016-08-23;11(8):e0161433.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  38. Impact of early cART in the gut during acute HIV infection
    Authors: Claire Deleage, Alexandra Schuetz, W. Gregory Alvord, Leslie Johnston, Xing-Pei Hao, David R. Morcock et al.
    JCI Insight
  39. Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues
    Lab Invest, 2016-06-27;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  40. Cardiac myosin-Th17 responses promote heart failure in human myocarditis
    Authors: Jennifer M. Myers, Leslie T. Cooper, David C. Kem, Stavros Stavrakis, Stanley D. Kosanke, Ethan M. Shevach et al.
    JCI Insight
  41. IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy
    Authors: Neal L Millar
    Sci Rep, 2016-06-06;6(0):27149.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  42. MRSA Infections in HIV-Infected People Are Associated with Decreased MRSA-Specific Th1 Immunity
    Authors: Netanya S. Utay, Annelys Roque, J. Katherina Timmer, David R. Morcock, Claire DeLeage, Anoma Somasunderam et al.
    PLOS Pathogens
  43. Development of Type 2, But Not Type 1, Leprosy Reactions is Associated with a Severe Reduction of Circulating and In situ Regulatory T-Cells
    Authors: Ana Paula Vieira, Maria Ângela Bianconcini Avancini, Carla Pagliari, João Avancini, Alberto José da Silva Sakai-Valente, Alberto José da Silva Sakai-Valente et al.
    The American Journal of Tropical Medicine and Hygiene
  44. A beneficial tumor microenvironment in oropharyngeal squamous cell carcinoma is characterized by a high T cell and low IL-17(+) cell frequency
    Authors: Simone Punt, Emilie A. C. Dronkers, Marij J. P. Welters, Renske Goedemans, Senada Koljenović, Elisabeth Bloemena et al.
    Cancer Immunology, Immunotherapy
  45. Association of Choroidal Interleukin-17-Producing T Lymphocytes and Macrophages with Geographic Atrophy
    Authors: Serge Camelo, Sophie Lavelette, Xavier Guillonneau, William Raoul, Florian Sennlaub
    Ophthalmologica
  46. Neuroendocrine tumors of the pancreas: a retrospective single-center analysis using the ENETS TNM-classification and immunohistochemical markers for risk stratification
    Authors: Stefan M Brunner, Florian Weber, Jens M Werner, Ayman Agha, Stefan A Farkas, Hans J Schlitt et al.
    BMC Surgery
  47. Selective inhibition of the p38 alternative activation pathway in infiltrating T cells inhibits pancreatic cancer progression
    Authors: Muhammad S. Alam, Matthias M. Gaida, Frank Bergmann, Felix Lasitschka, Thomas Giese, Nathalia A. Giese et al.
    Nature Medicine
  48. Association of Lung Inflammatory Cells with Small Airways Function and Exhaled Breath Markers in Smokers - Is There a Specific Role for Mast Cells?
    Authors: Nussbaumer-Ochsner Y, Stolk J, Ferraz da Silva L, van Schadewijk A, de Jeu R, Prins F, Mauad T, Rabe K, Hiemstra P
    PLoS ONE, 2015-06-12;10(6):e0129426.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  49. FoxP3(+) and IL-17(+) cells are correlated with improved prognosis in cervical adenocarcinoma
    Authors: Simone Punt, Marjolein E. van Vliet, Vivian M. Spaans, Cornelis D. de Kroon, Gert Jan Fleuren, Arko Gorter et al.
    Cancer Immunology, Immunotherapy
  50. RORgammat(+) hematopoietic cells are necessary for tumor cell proliferation during colitis-associated tumorigenesis in mice.
    Authors: Martin M, Kesselring R, Saidou B, Brunner S, Schiechl G, Mouris V, Wege A, Rummele P, Schlitt H, Geissler E, Fichtner-Feigl S
    Eur J Immunol, 2015-05-15;45(6):1667-79.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  51. Correlations between immune response and vascularization qRT-PCR gene expression clusters in squamous cervical cancer.
    Authors: Punt S, Houwing-Duistermaat J, Schulkens I, Thijssen V, Osse E, de Kroon C, Griffioen A, Fleuren G, Gorter A, Jordanova E
    Mol Cancer, 2015-03-31;14(0):71.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  52. Preferential recruitment of Th17 cells to cervical cancer via CCR6-CCL20 pathway.
    Authors: Yu, Qing, Lou, Xiang-mi, He, Yan
    PLoS ONE, 2015-03-13;10(3):e0120855.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  53. Upregulation of IL-17A/F from human lung tissue explants with cigarette smoke exposure: implications for COPD.
    Authors: Chang Y, Al-Alwan L, Alshakfa S, Audusseau S, Mogas A, Chouiali F, Nair P, Baglole C, Hamid Q, Eidelman D
    Respir Res, 2014-11-27;15(1):145.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  54. Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients.
    Authors: Liu, Xiaosun, Jin, Hailong, Zhang, Geer, Lin, Xianke, Chen, Chao, Sun, Jianyi, Zhang, Yu, Zhang, Qing, Yu, Jiren
    PLoS ONE, 2014-09-08;9(9):e106834.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  55. IL-17/Th17 pathway is activated in acne lesions.
    Authors: Kelhala H, Palatsi R, Fyhrquist N, Lehtimaki S, Vayrynen J, Kallioinen M, Kubin M, Greco D, Tasanen K, Alenius H, Bertino B, Carlavan I, Mehul B, Deret S, Reiniche P, Martel P, Marty C, Blume-Peytavi U, Voegel J, Lauerma A
    PLoS ONE, 2014-08-25;9(8):e105238.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  56. Allergic contact dermatitis in psoriasis patients: typical, delayed, and non-interacting.
    Authors: Quaranta M, Eyerich S, Knapp B, Nasorri F, Scarponi C, Mattii M, Garzorz N, Harlfinger A, Jaeger T, Grosber M, Pennino D, Mempel M, Schnopp C, Theis F, Albanesi C, Cavani A, Schmidt-Weber C, Ring J, Eyerich K
    PLoS ONE, 2014-07-24;9(7):e101814.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  57. Interleukin-17A promotes MUC5AC expression and goblet cell hyperplasia in nasal polyps via the Act1-mediated pathway.
    Authors: Xia, Wentong, Bai, Jing, Wu, Xingmei, Wei, Yi, Feng, Shaoyan, Li, Lei, Zhang, Jia, Xiong, Guanxia, Fan, Yunping, Shi, Jianbo, Li, Huabin
    PLoS ONE, 2014-06-03;9(6):e98915.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  58. Sera from remitting and secondary progressive multiple sclerosis patients disrupt the blood-brain barrier.
    Authors: Shimizu F, Tasaki A, Sano Y, Ju M, Nishihara H, Oishi M, Koga M, Kawai M, Kanda T
    PLoS ONE, 2014-03-31;9(3):e92872.
    Species: Human
    Sample Types: Serum
    Applications: Neutralization
  59. Th1 response and systemic treg deficiency in inclusion body myositis.
    Authors: Allenbach Y, Chaara W, Rosenzwajg M, Six A, Prevel N, Mingozzi F, Wanschitz J, Musset L, Charuel J, Eymard B, Salomon B, Duyckaerts C, Maisonobe T, Dubourg O, Herson S, Klatzmann D, Benveniste O
    PLoS ONE, 2014-03-04;9(3):e88788.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  60. The effect of adalimumab on key drivers in the pathogenesis of psoriasis.
    Authors: Hendriks A, van der Velden H, Wolberink E, Seyger M, Schalkwijk J, Zeeuwen P, de Jong E, Pasch M, van Erp P, van de Kerkhof P
    Br J Dermatol, 2014-03-01;170(3):571-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  61. IL-17A induces endothelial inflammation in systemic sclerosis via the ERK signaling pathway.
    Authors: Xing X, Yang J, Yang X, Wei Y, Zhu L, Gao D, Li M
    PLoS ONE, 2013-12-23;8(12):e85032.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC-P, Neutralization
  62. Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A.
    Authors: Lotti, Fiorenza, Jarrar, Awad M, Pai, Rish K, Hitomi, Masahiro, Lathia, Justin, Mace, Adam, Gantt, Gerald A, Sukhdeo, Kumar, DeVecchio, Jennifer, Vasanji, Amit, Leahy, Patrick, Hjelmeland, Anita B, Kalady, Matthew, Rich, Jeremy N
    J Exp Med, 2013-12-09;210(13):2851-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC, Neutralization
  63. In vivo induction of cutaneous inflammation results in the accumulation of extracellular trap-forming neutrophils expressing RORgammat and IL-17.
    Authors: Keijsers R, Hendriks A, van Erp P, van Cranenbroek B, van de Kerkhof P, Koenen H, Joosten I
    J Invest Dermatol, 2013-12-06;134(5):1276-84.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  64. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
    Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
    Cytokine, 2013-10-12;64(3):660-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  65. The Transcription Factor CREB Enhances Interleukin-17A Production and Inflammation in a Mouse Model of Atherosclerosis
    Authors: Sivareddy Kotla, Nikhlesh K. Singh, Mark R. Heckle, Gabor J. Tigyi, Gadiparthi N. Rao
    Science Signaling
  66. An interleukin-17–mediated paracrine network promotes tumor resistance to anti-angiogenic therapy
    Authors: Alicia S Chung, Xiumin Wu, Guanglei Zhuang, Hai Ngu, Ian Kasman, Jianhuan Zhang et al.
    Nature Medicine
  67. High IL-17-positive tumor immune cell infiltration is indicative for chemosensitivity of ovarian carcinoma
    Authors: Raoul A. Droeser, Uwe Güth, Serenella Eppenberger-Castori, Sylvia Stadlmann, Christian Hirt, Luigi Terracciano et al.
    Journal of Cancer Research and Clinical Oncology
  68. Chemokine (C-C Motif) Ligand 20, a Potential Biomarker for Graves' Disease, Is Regulated by Osteopontin
    Authors: Xiaoli Li, Yicheng Qi, Xinran Ma, Fengjiao Huang, Hua Guo, Xiaohua Jiang et al.
    PLoS ONE
  69. Interleukin-17A+ cell counts are increased in systemic sclerosis skin and their number is inversely correlated with the extent of skin involvement.
    Authors: Truchetet M, Brembilla N, Montanari E, Lonati P, Raschi E, Zeni S, Fontao L, Meroni P, Chizzolini C
    Arthritis Rheum, 2013-05-01;65(5):1347-56.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  70. Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
    Authors: Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam T, Leypoldt F, Simova O, Thom V, Friese M, Prinz I, Holscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T
    Blood, 2012-09-13;120(18):3793-802.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  71. Human CCR4+ CCR6+ Th17 cells suppress autologous CD8+ T cell responses
    Authors: Fei Zhao, Bastian Hoechst, Jaba Gamrekelashvili, Lars A. Ormandy, Torsten Voigtländer, Heiner Wedemeyer et al.
    The Journal of Immunology
  72. Differential pattern and prognostic significance of CD4+, FOXP3+ and IL-17+ tumor infiltrating lymphocytes in ductal and lobular breast cancers.
    Authors: Droeser R, Zlobec I, Kilic E, Guth U, Heberer M, Spagnoli G, Oertli D, Tapia C
    BMC Cancer, 2012-04-03;12(0):134.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  73. RASA1 maintains the lymphatic vasculature in a quiescent functional state in mice.
    Authors: Lapinski PE, Kwon S, Lubeck BA
    J. Clin. Invest., 2012-01-09;122(2):733-47.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC-P, Neutralization
  74. Interleukin-17 expression in neutrophils and Th17 cells in cutaneous T-cell lymphoma associated with neutrophilic infiltrate of the skin.
    Authors: Fontao L, Brembilla NC, Masouye I, Kaya G, Prins C, Dupin N, Saurat JH, Chizzolini C, Piguet V
    Br. J. Dermatol., 2012-01-09;166(3):687-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  75. Induction of endothelial nitric oxide synthase expression by IL-17 in human vascular endothelial cells: implications for vascular remodeling in transplant vasculopathy.
    Authors: Liu AC, Lee M, McManus BM, Choy JC
    J. Immunol., 2012-01-04;188(3):1544-50.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Neutralization
  76. MIP-3 alpha Expression in Macrophages Is NOD Dependent
    Authors: M. Hausmann, C. Zeitler, A. Weber, M. Krebs, S. Kellermeier, P. Rosenstiel et al.
    Digestion
  77. CD8 positive T cells express IL-17 in patients with chronic obstructive pulmonary disease
    Authors: Ying Chang, Jessica Nadigel, Nicholas Boulais, Jean Bourbeau, François Maltais, David H Eidelman et al.
    Respiratory Research
  78. IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
    Clin. Cancer Res., 2011-11-02;17(24):7529-38.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  79. The Th17 Pathway in Cystic Fibrosis Lung Disease
    Authors: Hui-Leng Tan, Nicolas Regamey, Sarah Brown, Andrew Bush, Clare M. Lloyd, Jane C. Davies
    American Journal of Respiratory and Critical Care Medicine
  80. Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
    Authors: Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C
    Br. J. Dermatol., 2011-05-17;164(6):1228-34.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  81. Delineation of a cellular hierarchy in lung cancer reveals an oncofetal antigen expressed on tumor-initiating cells.
    Authors: Damelin M, Geles KG, Follettie MT, Yuan P, Baxter M, Golas J, Dijoseph JF, Karnoub M, Huang S, Diesl V, Behrens C, Choe SE, Rios C, Gruzas J, Sridharan L, Dougher M, Kunz A, Hamann PR, Evans D, Armellino D, Khandke K, Marquette K, Tchistiakova L, Boghaert ER, Abraham RT, Wistuba II, Zhou BB
    Cancer Res., 2011-05-03;71(12):4236-46.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  82. Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis.
    Authors: Res PC, Piskin G, de Boer OJ, van der Loos CM, Teeling P, Bos JD, Teunissen MB
    PLoS ONE, 2010-11-24;5(11):e14108.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  83. Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD.
    Authors: Doe C, Bafadhel M, Siddiqui S, Desai D, Mistry V, Rugman P, McCormick M, Woods J, May R, Sleeman MA, Anderson IK, Brightling CE
    Chest, 2010-06-10;138(5):1140-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  84. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010-03-31;184(9):4880-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  85. Engineering of stable bispecific antibodies targeting IL-17A and IL-23
    Authors: Robert Mabry, Katherine E. Lewis, Margaret Moore, Patricia A. McKernan, Thomas R. Bukowski, Kristen Bontadelli et al.
    Protein Engineering, Design and Selection
  86. IL-17 induces monocyte migration in rheumatoid arthritis.
    Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
    J. Immunol., 2009-03-15;182(6):3884-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  87. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2008-12-03;123(1):59-66.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  88. Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides.
    Authors: Jandus C, Bioley G, Rivals JP, Dudler J, Speiser D, Romero P
    Arthritis Rheum., 2008-08-01;58(8):2307-17.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  89. Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers.
    Authors: Nistala K, Moncrieffe H, Newton KR, Varsani H, Hunter P, Wedderburn LR
    Arthritis Rheum., 2008-03-01;58(3):875-87.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  90. Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum.
    Authors: Witowski J, Ksiazek K, Warnecke C, Kuzlan M, Korybalska K, Tayama H, Wisniewska-Elnur J, Pawlaczyk K, Trominska J, Breborowicz A, Jorres A
    Kidney Int., 2007-01-17;71(6):514-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  91. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005-11-01;175(9):6177-89.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  92. Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
    Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
    J. Immunol., 2005-02-15;174(4):2376-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  93. Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
    Authors: Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P
    Am. J. Pathol., 2004-02-01;164(2):409-17.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  94. Interleukin-17 induces angiogenesis in�vitro via CXCL8 and CCL2 in retinal pigment epithelium
    Authors: Y Chen, M Zhong, G Yuan, H Peng
    Mol Med Rep, 2018-01-18;0(0):.

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Human IL-17/IL-17A Antibody
By Anonymous on 09/13/2023
Application: IHC Sample Tested: Tonsil tissue Species: Human

FFPE heating antigen retrieval in pH9
primary Ab x100 in 1% BSA (IgG free) for 1 hour
biotinylated rabbit anti-goat and streptavidin-HRP + DAB

Human IL-17/IL-17A Antibody
By Stephanie Zandee on 04/13/2018
Application: IHC Sample Tested: Adult brain Species: Human

Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287
Used at 10ug/ml.
Briefly, frozen brain sections were fixed in 4% PFA (Fisher Scientific), followed by antigen retrieval using heating in acid citric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector). Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc Receptor Blocking Solution was added (Human TruStain FcX Biolegend,
London, UK). Primary antibody was added overnight at 4 °C and detected with donkey anti-goat-biotin (ab6578, Abcam), followed by streptavidin-alkaline phosphatase (SA-5100, Vector) and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit III (Vector).

Immunohistochemistry Human IL-17/IL-17A Antibody AF-317-NA
Human IL-17/IL-17A Antibody
By BAREUN KIM on 03/23/2018
Application: B/N Sample Tested: 4T1 mouse breast cancer cell line,Breast tissue Species: Mouse and mouse tumor-infiltrate lympocytes
Human IL‑17/IL‑17A Antibody
By Nana Feinberg on 04/21/2017

Antigen retrieval pH 6.0 (20 min)
Dilution-1:100, 1 h incubation.
Detection with ImmPRESS HRP Anti-Goat Ig (Peroxidase) Polymer (# MP-7405-15 ) and TSA-Cy3 (#SAT704A001EA)

Human IL‑17/IL‑17A Antibody AF-317-NA
Human IL-17/IL-17A Antibody
By Nana Feinberg on 04/21/2017
Application: IHC Sample Tested: Colon tissue,Spleen tissue Species: Human

Staining is done in Leica Bond autostainer. Epitope retrieval at pH 6 (20 min), IL17/IL17A dilution 1:100, incubation time-1h. Detected with TSA-Cy3

Immunohistochemistry Human IL-17/IL-17A Antibody AF-317-NA