Human IL-15R alpha Biotinylated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
Background: IL-15R alpha
Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL‑15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL‑15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 aa protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL‑15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL‑15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.
- Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
- Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
- Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
- Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.
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Citations for Human IL-15R alpha Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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How a cytokine is chaperoned through the secretory pathway by complexing with its own receptor: lessons from interleukin-15 (IL-15)/IL-15 receptor alpha.
Authors: Duitman EH, Orinska Z, Bulanova E, Paus R, Bulfone-Paus S
Mol. Cell. Biol., 2008-05-27;28(15):4851-61.
Species: Human
Sample Types: Cell Culture Supernates, Whole Cells
Applications: ELISA Development, Flow Cytometry -
Tumor growth impedes natural-killer-cell maturation in the bone marrow.
Authors: Richards JO, Chang X, Blaser BW, Caligiuri MA, Zheng P, Liu Y
Blood, 2006-03-23;108(1):246-52.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-15 and cognate antigen successfully expand de novo-induced human antigen-specific regulatory CD4+ T cells that require antigen-specific activation for suppression.
Authors: Koenen HJ, Fasse E, Joosten I
J. Immunol., 2003-12-15;171(12):6431-41.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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Detection of Fc-IL15/IL15Ra by ELISA
