Human IL-15R alpha Antibody

Catalog #: MAB147
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB147
MAB147-SP

All IL-15R alpha Antibodies

Product Details

Citations (5)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-15R alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-15 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL‑15 R alpha, recombinant human (rh) IL-2 R alpha, rhIL-2 R beta, or rhIL-2 R gamma is observed.

Source

Monoclonal Mouse IgG1 Clone # 151307

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑15 R alpha Fc Chimera (Catalog # 147-IR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-15R alpha

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells.
IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of
IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 aa protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma _c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

References

Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.

Long Name

Interleukin 15 Receptor alpha

Entrez Gene IDs

3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)

Alternate Names

CD215 antigen; CD215; IL15 R alpha; IL-15 R alpha; IL-15 receptor subunit alpha; IL-15R alpha; IL15RA; IL-15Ra; IL-15R-alpha; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; MGC104179

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-15R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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A Refractory Celiac Patient Successfully Treated With Mesenchymal Stem Cell Infusions
Authors: R Ciccociopp, A Gallia, MA Avanzini, E Betti, C Picone, A Vanoli, C Paganini, F Biagi, R Maccario, GR Corazza
Mayo Clin Proc, 2016-04-14;0(0):.
Species: Human
Sample Types: BALF
Applications: Western Blot
IL15RA drives antagonistic mechanisms of cancer development and immune control in lymphocyte-enriched triple-negative breast cancers.
Authors: Marra P, Mathew S, Grigoriadis A, Wu Y, Kyle-Cezar F, Watkins J, Rashid M, De Rinaldis E, Hessey S, Gazinska P, Hayday A, Tutt A
Cancer Res, 2014-06-30;74(17):4908-21.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
PLoS ONE, 2012-07-09;7(7):e40620.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
EBV-associated mononucleosis does not induce long-term global deficit in T-cell responsiveness to IL-15.
Authors: Giron-Michel J, Menard F, Negrini S, Devocelle A, Azzarone B, Besson C
Blood, 2009-03-04;113(19):4541-7.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot
Phase I trial of a CD8+ T-cell peptide epitope-based vaccine for infectious mononucleosis.
Authors: Elliott SL, Suhrbier A, Miles JJ
J. Virol., 2007-11-21;82(3):1448-57.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry