Human IL-12 Antibody Summary
Arg23-Ser219 of p35; Ile23-Ser328 of p40
Accession # P29459 (p35) & P29460 (p40)
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by IL‑12 and Neutralization by Human IL‑12 Antibody. Recombinant Human IL‑12 (Catalog # 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑12 Polyclonal Antibody (Catalog # AB-219-NA). The ND50 is typically 1-2 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12
Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.
IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.
Product Datasheets
Citations for Human IL-12 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Interferon-alpha and interleukin-12 are induced differentially by Toll-like receptor 7 ligands in human blood dendritic cell subsets
Authors: Tomoki Ito, Ryuichi Amakawa, Tsuneyasu Kaisho, Hiroaki Hemmi, Kenichirou Tajima, Kazutaka Uehira et al.
The Journal of Experimental Medicine
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mWTX-330, an IL 12 INDUKINE Molecule, Activates and Reshapes Tumor-infiltrating CD8+ T and NK Cells to Generate Antitumor Immunity
Authors: CJ Nirschl, HR Brodkin, C Domonkos, CJ Dwyer, DJ Hicklin, N Ismail, C Seidel-Dug, P Steiner, Z Steuert, JM Sullivan, WM Winston, A Salmeron
Cancer Immunology Research, 2023-04-19;0(0):.
Species: Human
Sample Types: Serum
Applications: Western Blot -
Nucleolar protein NOP2/NSUN1 suppresses HIV-1 transcription and promotes viral latency by competing with Tat for TAR binding and methylation
Authors: W Kong, A Biswas, D Zhou, G Fiches, K Fujinaga, N Santoso, J Zhu
PLoS Pathog., 2020-03-16;16(3):e1008430.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
A Novel Bromodomain Inhibitor Reverses HIV-1 Latency through Specific Binding with BRD4 to Promote Tat and P-TEFb Association
Authors: H Huang, S Liu, M Jean, S Simpson, H Huang, M Merkley, T Hayashi, W Kong, I Rodríguez-, X Zhang, HO Yosief, H Miao, J Que, JJ Kobie, J Bradner, NG Santoso, W Zhang, J Zhu
Front Microbiol, 2017-06-07;8(0):1035.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
IL-33 induces IL-9 production in human CD4+ T cells and basophils.
Authors: Blom L, Poulsen BC, Jensen BM, Hansen A, Poulsen LK
PLoS ONE, 2011-07-06;6(7):e21695.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes
Authors: Fudi Zhong, Nan Zhou, Kang Wu, Yubiao Guo, Weiping Tan, Hong Zhang et al.
Nucleic Acids Research
FAQs
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Does the human IL-12 polyclonal antibody (Catalog # AB-219-NA) recognize IL-12 p70?
In Western blots, this antibody recognizes the p70 heterodimer as well as the p40 and p35 subunits.
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