Human IGFBP-3 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 4.13 | 12.7 | 31.1 | 4.06 | 11.8 | 28.5 |
Standard Deviation | 0.2 | 0.64 | 0.71 | 0.22 | 0.76 | 2.27 |
CV% | 4.8 | 5 | 2.3 | 5.4 | 6.4 | 8 |
Recovery
The recovery of IGFBP-3 spiked to three different levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 94 | 89-99 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: IGFBP-3
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human IGFBP-3 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Effects of high-intensity interval training on cardiometabolic biomarkers in patients with prostate cancer undergoing active surveillance: a randomized controlled trial
Authors: Kang, DW;Field, CJ;Patel, D;Fairey, AS;Boulé, NG;Dieli-Conwright, CM;Courneya, KS;
Prostate cancer and prostatic diseases
Species: Human
Sample Types: Serum
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Upregulation of Anti-Angiogenic miR-106b-3p Correlates Negatively with IGF-1 and Vascular Health Parameters in a Model of Subclinical Cardiovascular Disease: Study with Metformin Therapy
Authors: Bakhashab, S;O'Neill, J;Barber, R;Arden, C;Weaver, JU;
Biomedicines
Species: Human
Sample Types: Serum
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Association Study of IGF-1 rs35767 and rs6214 Gene Polymorphisms with Cancer Susceptibility and Circulating Levels of IGF-1, IGFBP-2, and IGFBP-3 in Colorectal Cancer Patients
Authors: Alrashid, MH;Al-Serri, AE;Hussain, RF;Al-Bustan, SA;Al-Barrak, J;
Biomedicines
Species: Human
Sample Types: Plasma
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Obese Asthma Phenotype Is Associated with hsa-miR-26a-1-3p and hsa-miR-376a-3p Modulating the IGF Axis
Authors: Gil-Martínez, M;Lorente-Sorolla, C;Rodrigo-Muñoz, JM;Naharro, S;García-de Castro, Z;Sastre, J;Valverde-Monge, M;Quirce, S;Caballero, ML;Olaguibel, JM;Del Pozo, V;
International journal of molecular sciences
Species: Human
Sample Types: Serum
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Selected Parameters of Bone Turnover in Neuroendocrine Tumors-A Potential Clinical Use?
Authors: Strzelczyk, J;Wójcik-Giertuga, M;Strzelczyk, JK;Seńkowska, AP;Biernacki, K;Kos-Kudła, B;
Journal of clinical medicine
Species: Human
Sample Types: Serum
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Energy Homeostasis Gene Nucleotide Variants and Survival of Hemodialysis Patients-A Genetic Cohort Study
Authors: MK ?widerska, A Mostowska, D Skrypnik, PP Jagodzi?sk, P Bogda?ski, AE Grzegorzew
Journal of Clinical Medicine, 2022-09-18;11(18):.
Species: Human
Sample Types: Plasma
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IGFBP-3 Regulates Mitochondrial Hyperfusion and Metabolic Activity in Ocular Surface Epithelia during Hyperosmolar Stress
Authors: WL Stuard, MK Guner, DM Robertson
International Journal of Molecular Sciences, 2022-04-06;23(7):.
Species: Human
Sample Types: Conditioned Media
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Visceral Adiposity, Inflammation, and Testosterone Predict Skeletal Muscle Mitochondrial Mass and Activity in Chronic Spinal Cord Injury
Authors: JA Goldsmith, RE Lai, RS Garten, Q Chen, EJ Lesnefsky, RA Perera, AS Gorgey
Frontiers in Physiology, 2022-02-10;13(0):809845.
Species: Human
Sample Types: Serum
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IGFBP-3 Mediates Metabolic Homeostasis During Hyperosmolar Stress in the Corneal Epithelium
Authors: ED Bogdan, WL Stuard, R Titone, DM Robertson
Investigative Ophthalmology & Visual Science, 2021-06-01;62(7):11.
Species: Human
Sample Types: Cell Lysates
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Discovery and validation of PZP as a novel serum biomarker for screening lung adenocarcinoma in type 2 diabetes mellitus patients
Authors: J Yang, C Yang, H Shen, W Wu, Z Tian, Q Xu, C Cao, S Ye, L Ban, X Tong, J Mei
Cancer Cell International, 2021-03-10;21(1):162.
Species: Human
Sample Types: Serum
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Circulating growth factor concentrations and breast cancer risk: a nested case-control study of IGF-1, IGFBP-3, and breast cancer in a family-based cohort
Authors: KR Monson, M Goldberg, HC Wu, RM Santella, WK Chung, MB Terry
Breast Cancer Res, 2020-10-22;22(1):109.
Species: Human
Sample Types: Plasma
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High intensity interval training combined with L-citrulline supplementation: Effects on physical performance in healthy older adults
Authors: F Buckinx, LP Carvalho, V Marcangeli, M Dulac, G Hajj Boutr, G Gouspillou, P Gaudreau, P Noirez, M Aubertin-L
Exp. Gerontol., 2020-07-25;0(0):111036.
Species: Human
Sample Types: Serum
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Physiological Disturbance in Fatty Liver Energy Metabolism Converges on IGFBP2 Abundance and Regulation in Mice and Men
Authors: P Fahlbusch, B Knebel, T Hörbelt, DM Barbosa, A Nikolic, S Jacob, H Al-Hasani, F Van de Vel, Y Van Nieuwe, D Müller-Wie, B Lapauw, DM Ouwens, J Kotzka
Int J Mol Sci, 2020-06-10;21(11):.
Species: Human
Sample Types: Serum
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PM2.5-bound PAHs exposure linked with low plasma insulin-like growth factor 1 levels and reduced child height
Authors: Z Zeng, X Huo, Q Wang, C Wang, MN Hylkema, X Xu
Environ Int, 2020-03-18;138(0):105660.
Species: Human
Sample Types: Plasma
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Enhanced Expression of IGFBP-3 Reduces Radiosensitivity and Is Associated with Poor Prognosis in Oral Squamous Cell Carcinoma
Authors: J Sakata, A Hirosue, R Yoshida, Y Matsuoka, K Kawahara, H Arita, H Nakashima, T Yamamoto, M Nagata, S Kawaguchi, S Gohara, Y Nagao, K Yamana, R Toya, R Murakami, Y Kuwahara, M Fukumoto, H Nakayama
Cancers (Basel), 2020-02-20;12(2):.
Species: Human
Sample Types: Plasma
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Early-Life Cadmium Exposure and Bone-Related Biomarkers: A Longitudinal Study in Children
Authors: A Malin Igra, M Vahter, R Raqib, M Kippler
Environ. Health Perspect., 2019-03-01;127(3):37003.
Species: Human
Sample Types: Plasma
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Hypoallergenic and anti-inflammatory feeds in children with complicated severe acute malnutrition: an open randomised controlled 3-arm intervention trial in Malawi
Authors: RH Bartels, E Chimwezi, V Watson, L Pei, I Potani, B Allubha, K Chidzalo, D Wang, Q Dube, M Mallewa, A Allen, RHJ Bandsma, WP Voskuijl, SJ Allen
Sci Rep, 2019-02-19;9(1):2304.
Species: Human
Sample Types: Plasma
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The effects of a combined bodyweight-based and elastic bands resistance training, with or without protein supplementation, on muscle mass, signaling and heat shock response in healthy older people
Authors: M Krause, D Crognale, K Cogan, S Contarelli, B Egan, P Newsholme, G De Vito
Exp. Gerontol., 2018-12-05;115(0):104-113.
Species: Human
Sample Types: Plasma
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Increases in IGF-1 after Anti-TNF-? Therapy are Associated with Bone and Muscle Accrual in Pediatric Crohn's Disease
Authors: MD DeBoer, AM Lee, K Herbert, J Long, M Thayu, LM Griffin, RN Baldassano, LA Denson, BS Zemel, MR Denburg, R Herskovitz, MB Leonard
J. Clin. Endocrinol. Metab., 2018-03-01;0(0):.
Species: Human
Sample Types: Serum
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Concurrent Aerobic and Resistance Training Has Anti-Inflammatory Effects and Increases Both Plasma and Leukocyte Levels of IGF-1 in Late Middle-Aged Type 2 Diabetic Patients
Authors: G Annibalini, F Lucertini, D Agostini, L Vallorani, A Gioacchini, E Barbieri, M Guescini, L Casadei, A Passalia, M Del Sal, G Piccoli, M Andreani, A Federici, V Stocchi
Oxid Med Cell Longev, 2017-06-21;2017(0):3937842.
Species: Human
Sample Types: Plasma
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A phase II randomized, double-blind, presurgical trial of Polyphenon E in bladder cancer patients to evaluate pharmacodynamics and bladder tissue biomarkers
Authors: JR Gee, DR Saltzstein, K Kim, JM Kolesar, W Huang, TC Havighurst, BW Wollmer, J Stublaski, T Downs, H Mukhtar, M House, HL Parnes, HH Bailey
Cancer Prev Res (Phila), 2017-03-21;0(0):.
Species: Human
Sample Types: Serum
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CD44 expression in endothelial colony-forming cells regulates neurovascular trophic effect
Authors: S Sakimoto, V Marchetti, E Aguilar, K Lee, Y Usui, S Murinello, F Bucher, JK Trombley, R Fallon, R Wagey, C Peters, EL Scheppke, PD Westenskow, M Friedlande
JCI Insight, 2017-01-26;2(2):e89906.
Species: Human
Sample Types: Cell Culture Supernates
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Relationships of insulin-like growth factor-1, its binding proteins, and cardiometabolic risk in hypertensive perimenopausal women
Authors: A Olszanecka, A Dragan, K Kawecka-Ja, D Fedak, D Czarnecka
Metab. Clin. Exp., 2017-01-12;69(0):96-106.
Species: Human
Sample Types: Plasma
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Genetic variation in IGF1 predicts renal cell carcinoma susceptibility and prognosis in Chinese population
Sci Rep, 2016-12-15;6(0):39014.
Species: Human
Sample Types: Serum
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Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells
Authors: Ge Sheng Cheng
Int. J. Mol. Med, 2016-12-07;39(1):223-230.
Species: Human
Sample Types: Cell Culture Supernates
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Elevations in growth hormone and glucagon-like peptide-2 levels on admission are associated with increased mortality in trauma patients.
Authors: Matthew P Rowan, Darrick J Beckman, Julie A Rizzo, Claire L Isbell, Christopher E White, Stephen M Cohn, Kevin K Chung
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, 2016-10-04;0(0):1757-7241.
Species: Human
Sample Types: Serum
Applications: ELISA Capture -
Phase IIa, randomized placebo-controlled trial of single high dose cholecalciferol (vitamin D3) and daily Genistein (G-2535) versus double placebo in men with early stage prostate cancer undergoing prostatectomy
Authors: Howard H Bailey
Am J Clin Exp Urol, 2016-09-20;4(2):17-27.
Species: Human
Sample Types: Serum
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The Differential Hormonal Milieu of Morning versus Evening May Have an Impact on Muscle Hypertrophic Potential
PLoS ONE, 2016-09-01;11(9):e0161500.
Species: Human
Sample Types: Serum
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IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma
Authors: Matthew N Svalina
Sci Rep, 2016-06-03;6(0):27012.
Species: Human
Sample Types: CSF
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Effects of exercise and milk fat globule membrane (MFGM) supplementation on body composition, physical function, and hematological parameters in community-dwelling frail Japanese women: a randomized double blind, placebo-controlled, follow-up trial.
Authors: Kim H, Suzuki T, Kim M, Kojima N, Ota N, Shimotoyodome A, Hase T, Hosoi E, Yoshida H
PLoS ONE, 2015-02-06;10(2):e0116256.
Species: Human
Sample Types: Serum
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Glucose intolerance in aging male IGFBP-3 transgenic mice: differential effects of human IGFBP-3 and its mutant IGFBP-3 devoid of IGF binding ability.
Authors: Nguyen K, Yao X, Erickson A, Mishra S, Nyomba B
Endocrinology, 2014-12-09;156(2):462-74.
Species: Human
Sample Types: Serum
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Role of IGF-binding protein 3 in the resistance of EGFR mutant lung cancer cells to EGFR-tyrosine kinase inhibitors.
Authors: Choi Y, Park G, Rho J, Kim S, So G, Kim H, Choi C, Lee J
PLoS ONE, 2013-12-05;8(12):e81393.
Species: Human
Sample Types: Cell Culture Supernates
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Role of serum concentration of VEGFR1 and TIMP2 on clinical outcome in primary cervical cancer: results of a companion protocol of the randomized, NOGGO-AGO phase III adjuvant trial of simultaneous cisplatin-based radiochemotherapy vs. carboplatin and paclitaxel containing sequential radiotherapy.
Authors: Braicu E, Fotopoulou C, Chekerov R, Richter R, Blohmer J, Kummel S, Stamatian F, Yalcinkaya I, Mentze M, Lichtenegger W, Sehouli J
Cytokine, 2013-02-14;61(3):755-8.
Species: Human
Sample Types: Serum
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FOXA1 promotes tumor progression in prostate cancer via the insulin-like growth factor binding protein 3 pathway.
Authors: Imamura, Yusuke, Sakamoto, Shinichi, Endo, Takumi, Utsumi, Takanobu, Fuse, Miki, Suyama, Takahito, Kawamura, Koji, Imamoto, Takashi, Yano, Kojiro, Uzawa, Katsuhir, Nihei, Naoki, Suzuki, Hiroyosh, Mizokami, Atsushi, Ueda, Takeshi, Seki, Naohiko, Tanzawa, Hideki, Ichikawa, Tomohiko
PLoS ONE, 2012-08-03;7(8):e42456.
Species: Human
Sample Types: Cell Culture Supernates
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Plasminogen activator inhibitor 1--insulin-like growth factor binding protein 3 cascade regulates stress-induced senescence.
Authors: Elzi, David J, Lai, Yanlai, Song, Meihua, Hakala, Kevin, Weintraub, Susan T, Shiio, Yuzuru
Proc Natl Acad Sci U S A, 2012-07-09;109(30):12052-7.
Species: Human
Sample Types: Cell Culture Supernates
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Administration of omega-3 fatty acids and Raloxifene to women at high risk of breast cancer: interim feasibility and biomarkers analysis from a clinical trial.
Eur J Clin Nutr, 2012-06-06;66(8):878-84.
Species: Human
Sample Types: Serum
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Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis.
Authors: Weimer A, Madry H, Venkatesan JK
Mol. Med., 2012-05-09;18(1):346-58.
Species: Human
Sample Types: Cell Culture Supernates
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Maternal and fetal placental growth hormone and IGF axis in type 1 diabetic pregnancy.
Authors: Higgins MF, Russell NE, Crossey PA, Nyhan KC, Brazil DP, McAuliffe FM
PLoS ONE, 2012-02-17;7(2):e29164.
Species: Human
Sample Types: Plasma
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Prognostic significance of angiogenic factors in uterine cervical cancer.
Authors: Landt S, Wehling M, Heidecke H, Jeschke S, Korlach S, Stoblen F, Schmid P, Blohmer JU, Lichtenegger W, Sehouli J, Kummel S
Anticancer Res., 2011-08-01;31(8):2589-95.
Species: Human
Sample Types: Serum
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The stature of boys is inversely correlated to the levels of their sertoli cell hormones: do the testes restrain the maturation of boys?
Authors: Morgan K, Dennis NA, Ruffman T, Bilkey DK, McLennan IS
PLoS ONE, 2011-06-02;6(6):e20533.
Species: Human
Sample Types: Serum
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Biological effects induced by insulin-like growth factor binding protein 3 (IGFBP-3) in malignant melanoma.
Authors: Oy GF, Slipicevic A, Davidson B, Solberg Faye R, Maelandsmo GM, Florenes VA
Int. J. Cancer, 2010-01-15;126(2):350-61.
Species: Human
Sample Types: Plasma
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Abnormalities in metalloproteinase pathways and IGF-I axis: a link between birth weight, hypertension, and vascular damage in childhood.
Authors: Sesso R, Franco MC
Am. J. Hypertens., 2009-11-05;23(1):6-11.
Species: Human
Sample Types: Plasma
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Role of insulin-like growth factor binding protein-3 in allergic airway remodeling.
Authors: Veraldi KL, Gibson BT, Yasuoka H, Myerburg MM, Kelly EA, Balzar S, Jarjour NN, Pilewski JM, Wenzel SE, Feghali-Bostwick CA
Am. J. Respir. Crit. Care Med., 2009-07-16;180(7):611-7.
Species: Human
Sample Types: BALF
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Grape seed proanthocyanidins inhibit the growth of human non-small cell lung cancer xenografts by targeting insulin-like growth factor binding protein-3, tumor cell proliferation, and angiogenic factors.
Authors: Akhtar S, Meeran SM, Katiyar N, Katiyar SK
Clin. Cancer Res., 2009-02-01;15(3):821-31.
Species: Human
Sample Types: Plasma
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Serum insulin-like growth factor I (IGF-I) and IGF-binding protein 3 (IGFBP-3) in IVF patients with polycystic ovary syndrome: correlations with outcome.
Authors: Schoyer KD, Liu HC, Witkin S, Rosenwaks Z, Spandorfer SD
Fertil. Steril., 2007-02-22;88(1):139-44.
Species: Human
Sample Types: Serum
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Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy.
Authors: Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A
Clin. Cancer Res., 2007-02-01;13(3):1000-9.
Species: Human
Sample Types: Plasma
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Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo development.
Authors: Wang TH, Chang CL, Wu HM, Chiu YM, Chen CK, Wang HS
Fertil. Steril., 2006-11-01;86(5):1392-401.
Species: Human
Sample Types: Follicular Fluid
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