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Human IGF-II/IGF2 Quantikine ELISA Kit

Catalog #: DG200
7 Citations Datasheet / COA / SDS
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DG200
Control Products Available
QC230: Quantikine Immunoassay Control Set 1232 for Human IGF-II
Human IGF-II ELISA Standard Curve
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Citations (7)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IGF-II/IGF2 Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (25 uL)
Sensitivity
5.41 pg/mL
Assay Range
12.5 - 800 pg/mL (Serum-free Cell Culture Supern, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human IGF-II.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human IGF-II Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-II in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human IGF-II and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-II showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGF-II.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum-free Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 128 305 566 136 307 558
Standard Deviation 4.05 7.2 15.6 9.89 28.5 28.2
CV% 3.2 2.4 2.8 7.3 9.3 5.1

Recovery

The recovery of human IGF-II spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum-free Cell Culture Media (n=4) 95 82-104
Urine (n=4) 94 79-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IGF-II were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IGF-II ELISA Linearity

Scientific Data

N/A Human IGF-II ELISA Standard Curve View Larger

Human IGF-II ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-II/IGF2

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.

The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.

Long Name:
Insulin-like Growth Factor II
Entrez Gene IDs:
3481 (Human); 16002 (Mouse)
Alternate Names:
C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; GRDF; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PEG2; PP9974; somatomedin-A
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IGF-II/IGF2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Human iPS-derived pre-epicardial cells direct cardiomyocyte aggregation expansion and organization in vitro
    Authors: JJ Tan, JP Guyette, K Miki, L Xiao, G Kaur, T Wu, L Zhu, KJ Hansen, KH Ling, DJ Milan, HC Ott
    Nature Communications, 2021-08-17;12(1):4997.
    Species: Human
    Sample Types: Cell Cultured Supernates
  2. Evaluation of neurotrophic factors and education level as predictors of cognitive decline in alcohol use disorder
    Authors: N Requena-Oc, P Araos, M Flores, N García-Mar, D Silva-Peña, J Aranda, P Rivera, JJ Ruiz, A Serrano, FJ Pavón, J Suárez, F Rodríguez
    Scientific Reports, 2021-08-02;11(1):15583.
    Species: Human
    Sample Types: Plasma
  3. Potential association of plasma lysophosphatidic acid (LPA) species with cognitive impairment in abstinent alcohol use disorders outpatients
    Authors: N García-Mar, N Pizarro, FJ Pavón, M Martínez-H, M Flores-Lóp, N Requena-Oc, P Araos, D Silva-Peña, J Suárez, LJ Santín, R de la Torr, F Rodríguez, A Serrano
    Sci Rep, 2020-10-13;10(1):17163.
    Species: Human
    Sample Types: Plasma
  4. IGF2 autocrine-mediated IGF1R activation is a clinically relevant mechanism of osimertinib resistance in lung cancer
    Authors: T Manabe, H Yasuda, H Terai, H Kagiwada, J Hamamoto, T Ebisudani, K Kobayashi, K Masuzawa, S Ikemura, I Kawada, Y Hayashi, K Fukui, K Horimoto, K Fukunaga, K Soejima
    Mol. Cancer Res., 2020-01-15;0(0):.
    Species: Human
    Sample Types: BALF
  5. Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters, and Serological Worsening Markers in Nonalcoholic Fatty Liver Disease Patients
    Authors: A Federico, M Dallio, M Masarone, AG Gravina, R Di Sarno, C Tuccillo, V Cossiga, S Lama, P Stiuso, F Morisco, M Persico, C Loguercio
    Oxid Med Cell Longev, 2019;2019(0):8742075.
    Species: Human
    Sample Types: Serum
  6. IGF2 mRNA binding protein-2 is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1
    Authors: N Dai, F Ji, J Wright, L Minichiell, RI Sadreyev, J Avruch
    Elife, 2017-07-28;6(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Phase IIa, randomized placebo-controlled trial of single high dose cholecalciferol (vitamin D3) and daily Genistein (G-2535) versus double placebo in men with early stage prostate cancer undergoing prostatectomy
    Authors: Howard H Bailey
    Am J Clin Exp Urol, 2016-09-20;4(2):17-27.
    Species: Human
    Sample Types: Serum

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