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Human ICAM-3/CD50 Antibody

Catalog #: BBA15
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BBA15
ICAM‑3/CD50 in Human PBMCs.
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Citations (1)
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Human ICAM-3/CD50 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAM‑3/CD50 in ELISAs and Western blots. ICAM‑3/CD50 was found not to recognize COS cells expressing the human
ICAM‑1 protein. The epitope for ICAM‑3/CD50 has been found to be located on domain 1, or the interface between domains 1 and 2 of the human ICAM‑3/CD50 molecule.
Source
Monoclonal Mouse IgG1 Clone # ICAM-3.3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human ICAM-3 Fc Chimera
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ICAM‑3/CD50 Fc Chimera (Catalog # 715-IC)
Flow Cytometry
0.25 µg/106 cells
Human peripheral blood monocytes
Immunoprecipitation
Vazeux, R. et al. (1992) Nature 360:485.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
Cattoretti, G. et al. (1992) J. Pathol. 168:357.
 

Human ICAM-3/CD50 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human ICAM‑3/CD50 Biotinylated Antibody (Catalog # BAF813)

Standard: Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF (Catalog # 715-IC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry ICAM-3/CD50 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

ICAM‑3/CD50 in Human PBMCs. ICAM-3/CD50 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human ICAM-3/CD50 Monoclonal Antibody (Catalog # BBA15) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-3/CD50

Intercellular Adhesion Molecule-3 (ICAM-3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha d/ beta 2. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.

References
  1. Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Intercellular Adhesion Molecule 3
Entrez Gene IDs
3385 (Human)
Alternate Names
CD50 antigen; CD50; CDW50CDw50; ICAM3; ICAM-3; ICAM-Rintercellular adhesion molecule-3; intercellular adhesion molecule 3

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Citation for Human ICAM-3/CD50 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Macrophage recognition of ICAM-3 on apoptotic leukocytes.
    Authors: Moffatt OD, Devitt A, Bell ED
    J. Immunol., 1999-06-01;162(11):6800-10.
    Species: Human
    Sample Types: Recombinant Protein, Whole Cells
    Applications: Adhesion Assay, Direct ELISA

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