Human ICAM-3/CD50 Antibody

Catalog #: MAB8131 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8131-100
MAB8131-SP

Detection of ICAM-3/CD50 in Human Blood Lymphocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ICAM-3/CD50 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ICAM-3/CD50 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 76203

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ICAM‑3/CD50
Gln30-His485
Accession # CAA49473

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

PBMC lymphocytes

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of human lymph node

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ICAM-3/CD50 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human ICAM-3/CD50 Monoclonal Antibody (Catalog # MAB8131) or (B) Mouse IgG1 Isotype Control (MAB002), followed by Allophycocyanin-conjugated anti-Mouse IgG secondary antibody (F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunohistochemistry

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ICAM‑3/CD50 in Human Lymph Node. ICAM‑3/CD50 was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human ICAM‑3/CD50 Monoclonal Antibody (Catalog # MAB8131) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-3/CD50

Intercellular Adhesion Molecule-3 (ICAM-3; also known as CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha _d/ beta ₂. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.

References