Human HM74A/PUMA-G/GPR109A PE-conjugated Antibody

Catalog #: FAB2760P Datasheet / COA / SDS

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FAB2760P

Detection of HM74A/PUMA-G/GPR109A in Human Blood Neutrophils by Flow Cytometry.

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Human HM74A/PUMA-G/GPR109A PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human HM74A/PUMA-G/GPR109A. Stains human HM74A/PUMA-G/GPR109A-transfected cells but not irrelevant transfectants. Cross-reactivity with HM74 (GPR109B) was not tested.

Source

Monoclonal Rat IgG_2B Clone # 245106

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human HM74A/PUMA-G/GPR109A
Met1-Pro363
Accession # Q8TDS4

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of HM74A/PUMA-G/GPR109A in Human Blood Neutrophils by Flow Cytometry. Human peripheral blood neutrophils were stained with (A) Rat Anti-Human HM74A/PUMA-G/GPR109A PE-conjugated Monoclonal Antibody (Catalog # FAB2760P) or (B) isotype control antibody (Catalog # IC013P) and Mouse anti-Human Fc gamma RIII (CD16) APC-conjugated Monoclonal Antibody (Catalog # FAB2546A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

Detection of HM74A/PUMA-G/GPR109A antibody in HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP antibody by Flow Cytometry.

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Detection of HM74A/PUMA-G/GPR109A in HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human HM74A/PUMA-G/GPR109A and eGFP were stained with (A) Rat Anti-Human HM74A/PUMA-G/GPR109A PE-conjugated Monoclonal Antibody (Catalog # FAB2760P) or (B) Isotype Control antibody (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: HM74A/PUMA-G/GPR109A

HM74A/GPR109A (also known as PUMA-G in mouse) is a 50-55 kDa 7-transmembrane protein member of a small subfamily within the GPCR #1 family. It is expressed on or in a wide variety of cells, including microglia, neutrophils, islet beta -cells, macrophages, adipocytes, retinal pigment epithelium and keratinocytes. Although HM74A is noted for its ability to bind niacin, it natural ligand is beta -hydroxybutyrate. Thus it may naturally act as a negative regulator of fatty acid release by fat, which is converted to ketone bodies by the liver, and which feedback on adipocytes. Full-length human HM74A shares 95% amino acid (aa) sequence identity with human HM74 and 81% aa sequence identity with mouse HM74A.

Long Name

G Protein-coupled Receptor HM74A/G Protein-coupled Receptor 109A

Entrez Gene IDs

338442 (Human); 80885 (Mouse)

Alternate Names

GPR109A; HCA2; HCAR2; HM74A; hydroxycarboxylic acid receptor 2; Niacr1; Nicotinic Acid Receptor; PUMAG