Human GPRC5B Antibody

Catalog #: MAB10253 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10253-100
MAB10253-SP

Detection of GPRC5B in HEK293 Human Cell Line Transfected with Human GPRC5B and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPRC5B Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPRC5B.

Source

Monoclonal Mouse IgG_2A Clone # 575926

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GPRC5B
Accession # Q9NZH0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPRC5B in HEK293 Human Cell Line Transfected with Human GPRC5B and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) GPRC5B or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human GPRC5B Monoclonal Antibody (Catalog # MAB10253) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB003, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPRC5B

G-protein coupled receptor family C group 5-member B (GPRC5B) is a retinoic acid-inducible protein that belongs to the seven-transmembrane domain family of receptors. Human GPRC5B shares approximately 85% aa sequence identity with mouse GPRC5B.

Although GPRC5B is considered an "orphan receptor" and its molecular function is not completely understood, it has been implicated in obesity-associated inflammatory signaling in adipocytes and in diet-induced insulin resistance. Consistent with that, CRISPR-Cas9-mediated knock-down of the GPRC5B gene allowed the identification of signaling pathways linking the GPRC5B receptor expression to beta -cell proliferation and apoptosis.

Long Name

G Protein-coupled Receptor, Family C, Group 5, Member B

Entrez Gene IDs

51704 (Human); 64297 (Mouse); 293546 (Rat)

Alternate Names

G protein-coupled receptor, family C, group 1, member B; G protein-coupled receptor, family C, group 5, member B; GPRC5B; RAIG2; RAIG-2A-69G12.1; RAIG2G-protein coupled receptor family C group 5 member B; retinoic acid responsive gene protein; Retinoic acid-induced gene 2 protein