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Human GM-CSF R alpha APC-conjugated Antibody

Catalog #: FAB706A
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB706A
Detection of GM‑CSF R alpha in Human Blood Monocytes by Flow Cytometry.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GM-CSF R alpha APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GM-CSF R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) M‑CSF R or rhGM‑CSF R beta  ( beta c) is observed.
Source
Monoclonal Mouse IgG1 Clone # 31916
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GM-CSF R alpha
Extracellular domain
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
Human peripheral blood monocytes and U937 human histiocytic lymphoma cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of GM‑CSF Ra antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of GM‑CSF R alpha in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human GM-CSF Ra APC-conjugated Monoclonal Antibody (Catalog # FAB706A, filled histogram) or isotype control antibody (IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of GM‑CSF R alpha in U937 cells by Flow Cytometry. U937 cells were stained with Mouse Anti-Human GM‑CSF R alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB706A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: GM-CSF R alpha

Granulocyte Macrophage Colony Stimulating Factor Receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1‑4). Mature human GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a 26 aa transmembrane segment, and a 54 aa cytoplasmic domain (5). Within the ECD, human GM-CSF R alpha shares approximately 33% aa sequence identity with mouse and rat GM-CSF R alpha. Alternate splicing of human GM-CSF R alpha generates several additional isoforms that lack the cytoplasmic and/or transmembrane regions. Soluble forms of the receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non-hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9‑11). The shared usage of beta c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).

References
  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
  3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
  4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
  5. Gearing, D.P. et al. (1989) EMBO J. 8:3667.
  6. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
  7. Raines, M.A. et al. (1991) Proc. Natl. Acad. Sci. 88:8203.
  8. Chiba, S. et al. (1990) Cell Regul. 1:327.
  9. Kitamura, T. et al. (1991) Proc. Natl. Acad. Sci. 88:5082.
  10. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
  11. Hoang, T. et al. (1993) J. Biol. Chem. 268:11881.
Long Name
Granulocyte Macrophage Growth Factor Receptor alpha
Entrez Gene IDs
1438 (Human); 12982 (Mouse); 102139775 (Cynomolgus Monkey)
Alternate Names
CD116 antigen; CD116; CDw116; colony stimulating factor 2 receptor alpha subunit; colony stimulating factor 2 receptor, alpha, low-affinity(granulocyte-macrophage); CSF2R; CSF2RA; CSF2RAX; CSF2RAY; CSF2RX; CSF2RY; GM-CSF R alpha; GM-CSF receptor alpha subunit; GMCSFR alpha; GMCSFR; GM-CSFRa; GM-CSF-R-alpha; GMCSFR-alpha; GMR; GMR-alpha; granulocyte-macrophage colony-stimulating factor receptor alpha chain; granulocyte-macrophage colony-stimulating factor receptor subunit alpha; MGC3848; MGC4838

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Citation for Human GM-CSF R alpha APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Neutrophil Extracellular Traps Reprogram IL-4/GM-CSF-Induced Monocyte Differentiation to Anti-inflammatory Macrophages
    Authors: AB Guimarães-, NC Rochael, F Oliveira, J Echevarria, EM Saraiva
    Front Immunol, 2017-05-17;8(0):523.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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