Human Glut1 APC-conjugated Antibody

Catalog #: FAB1418A
4 Citations Datasheet / COA / SDS

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FAB1418A

Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry.

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All Glut1 Antibodies

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glut1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.

Source

Monoclonal Mouse IgG_2B Clone # 202915

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Glut1
Met1-Val492
Accession # AAA52571

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 APC-conjugated Monoclonal Antibody (Catalog # FAB1418A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of Glut1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either (A) untreated or (B) cultured in nutrient-depleted media was stained with Mouse Anti-Human Glut1 APC-conjugated Monoclonal Antibody (Catalog # FAB1418A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glut1

Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8⁺ T cells, and erythrocytes (1‑4). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.

References

Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
Meuckler, M. et al. 1985, Science 229:941.
Jones, K.S. et al. 2006, J. Virol. 8291.
Takenouchi, N. et al. 2007, J. Virol. 1506.
Kinet, S. et al. 2007, Retrovirology 4:31.

Long Name

Glucose Transporter Type 1

Entrez Gene IDs

6513 (Human); 20525 (Mouse)

Alternate Names

Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis); CSE; DYT17; DYT18; DYT9; EIG12; Glucose transporter type 1, erythrocyte/brain; Glut1; GLUT-1; GLUT1DS; HepG2 glucose transporter; HTLVR; Human T-Cell Leukemia Virus (I and II) Receptor; MGC141895; MGC141896; PED; SLC2A1; solute carrier family 2 (facilitated glucose transporter), member 1; Solute Carrier Family 2 Member 1; solute carrier family 2, facilitated glucose transporter member 1

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human Glut1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Neutrophil extracellular traps induce tumor metastasis through dual effects on cancer and endothelial cells
Authors: Ze-Zhou Jiang, Zhi-Peng Peng, Xing-Chen Liu, Hao-Fan Guo, Meng-Meng Zhou, Da Jiang et al.
OncoImmunology
Induction of HIF-1? by HIV-1 Infection in CD4+ T Cells Promotes Viral Replication and Drives Extracellular Vesicle-Mediated Inflammation
Authors: G Duette, P Pereyra Ge, J Rubione, PS Perez, AL Landay, SM Crowe, Z Liao, KW Witwer, MP Holgado, J Salido, J Geffner, O Sued, CS Palmer, M Ostrowski
MBio, 2018-09-11;9(5):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Polymorphism rs1385129 Within Glut1 Gene SLC2A1 Is Linked to Poor CD4+ T Cell Recovery in Antiretroviral-Treated HIV+ Individuals
Authors: Jesse J. R. Masson, Catherine L. Cherry, Nicholas M. Murphy, Isabel Sada-Ovalle, Tabinda Hussain, Riya Palchaudhuri et al.
Frontiers in Immunology
Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection.
Authors: Palmer C, Ostrowski M, Gouillou M, Tsai L, Yu D, Zhou J, Henstridge D, Maisa A, Hearps A, Lewin S, Landay A, Jaworowski A, McCune J, Crowe S
AIDS, 2014-01-28;28(3):297-309.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry