Human Ghrelin/Obestatin Antibody

Catalog # Availability Size / Price Qty
MAB81492-100
MAB81492-SP
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Human Ghrelin/Obestatin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Ghrelin/Obestatin in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 226710
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human Ghrelin/Obestatin
Accession # Q9UBU3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Ghrelin/Obestatin Monoclonal Antibody (Catalog # MAB81491).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ghrelin/Obestatin

Mature Ghrelin peptides are the result of the clavage of the Ghrelin/Obestatin Prepropeptide, a 117 aa precursor peptide that is processed into three chains, Ghrelin-27 (aa 24-50), Ghrelin-28 (aa24-51) and Obestatin (aa76-98).  The prepropeptide is cleaved into proGhrelin, and then further processed into mature Ghrelin by prohormone convertases.  Mature Ghrelin is acylated with an N-octanoyl group on serine 3 which is required for receptor binding.  Ghrelin and Obestatin are predominantly synthesized in the gastric mucosa.  Ghrelin plays a role in growth factor release and appetite suppression as well as many other functions in a variety of organs.  Obestatin is a putative hormone that has been suggested to have opposite effects on growth factor release and appetite as Ghrelin.

Entrez Gene IDs
51738 (Human)
Alternate Names
appetite-regulating hormone; ghrelin; ghrelin/obestatin preprohormone; ghrelin/obestatin prepropeptide; Ghrelin/Obestatin; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; MTLRPgrowth hormone secretagogue receptor ligand; obestatin; Protein M46

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