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Human GAD2/GAD65 Antibody

Catalog #: AF2247
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2247
AF2247-SP
GAD2/GAD65 in Human Dorsal Root Ganglia.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GAD2/GAD65 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GAD2/GAD65 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human GAD1/GAD67 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GAD2/GAD65
Ala1-Ala150
Accession # Q05329
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human GAD2/GAD65
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry GAD2/GAD65 antibody in Human Dorsal Root Ganglia by Immunohistochemistry (IHC-P). View Larger

GAD2/GAD65 in Human Dorsal Root Ganglia. GAD2/GAD65 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using Goat Anti-Human GAD2/GAD65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2247) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal roots. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GAD2/GAD65

Glutamic acid decarboxylases (GAD), which catalyze the formation of gamma-aminobutyric acid from L-glutamic acid, are detectable in different isoforms encoded by distinct genes. GAD2, also known as GAD65, is expressed in the brain and pancreatic islets. GAD2 has been implicated as an autoantigen in the autoimmune disease Stiff Man Syndrome and in insulin-dependent diabetes mellitus.

Long Name
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)
Entrez Gene IDs
2572 (Human)
Alternate Names
EC 4.1.1; EC 4.1.1.15; GAD2; GAD65; GAD-65; GAD65MGC161605; glutamate decarboxylase 2 (pancreatic islets and brain, 65kD); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase-2 (pancreas); MGC161607,65 kDa glutamic acid decarboxylase

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Citations for Human GAD2/GAD65 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome
    Authors: Gucci Jijuan Gu Urban, Mikaela Friedman, Ping Ren, Carina Törn, Malin Fex, Christiane S. Hampe et al.
    Scientific Reports
  2. Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells
    Authors: Y Miura, MY Li, F Birey, K Ikeda, O Revah, MV Thete, JY Park, A Puno, SH Lee, MH Porteus, SP Pa?ca
    Nature Biotechnology, 2020-12-03;38(12):1421-1430.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Sensitive detection of multiple islet autoantibodies in type 1 diabetes using small sample volumes by agglutination-PCR
    Authors: FJ Cortez, D Gebhart, PV Robinson, D Seftel, N Pourmandi, J Owyoung, CR Bertozzi, DM Wilson, DM Maahs, BA Buckingham, JR Mills, MM Roforth, SJ Pittock, A McKeon, K Page, WA Wolf, S Sanda, C Speake, CJ Greenbaum, CT Tsai
    PLoS ONE, 2020-11-13;15(11):e0242049.
    Species: Human
    Sample Types: Serum
    Applications: Bioassay
  4. Prolonged human neural stem cell maturation supports recovery in injured rodent CNS
    Authors: P Lu, S Ceto, Y Wang, L Graham, D Wu, H Kumamaru, E Staufenber, MH Tuszynski
    J. Clin. Invest., 2017-08-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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