Human Free IGFBP-1 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 1038 | 3065 | 5639 | 1079 | 3100 | 5895 |
| Standard Deviation | 58.2 | 131 | 230 | 103 | 209 | 373 |
| CV% | 5.6 | 4.3 | 4.1 | 9.5 | 6.7 | 6.3 |
Recovery
The recovery of human Free IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 112 | 92-117 |
| Cell Lysates (n=4) | 107 | 91-117 |
| EDTA Plasma (n=4) | 97 | 86-111 |
| Heparin Plasma (n=4) | 97 | 85-108 |
| Human Milk (n=4) | 104 | 92-114 |
| Serum (n=4) | 94 | 86-107 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: IGFBP-1
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 3 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Free IGFBP-1 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
5
Citations: Showing 1 - 5
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Placental RNA sequencing implicates IGFBP1 in insulin sensitivity during pregnancy and in gestational diabetes
Authors: Hivert, MF;White, F;Allard, C;James, K;Majid, S;Aguet, F;Ardlie, K;Edlow, A;Florez, J;Bouchard, L;Jacques, PE;Karumanchi, S;Powe, C;
Research square
Species: Human
Sample Types: Plasma
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EGF, TGF-? and Amphiregulin Differently Regulate Endometrium-Derived Mesenchymal Stromal/Stem Cells
Authors: Kamentseva, RS;Kharchenko, MV;Gabdrahmanova, GV;Kotov, MA;Kosheverova, VV;Kornilova, ES;
International journal of molecular sciences
Species: Human
Sample Types: Cell Culture Supernates
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The fate of human SUSD2+ endometrial mesenchymal stem cells during decidualization
Authors: T Gorsek Spa, UR Markert, P Reif, W Schoell, G Moser, J Feichtinge, ZN Mihalic, J Kargl, CE Gargett, D Gold
Stem Cell Research, 2022-01-15;60(0):102671.
Species: Human
Sample Types: Cell Culture Supernates
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Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth
Authors: DH Bak, MJ Choi, SR Kim, BC Lee, JM Kim, ES Jeon, W Oh, ES Lim, BC Park, MJ Kim, J Na, BJ Kim
Korean J. Physiol. Pharmacol., 2018-08-27;22(5):555-566.
Species: Human
Sample Types: Cell Culture Supernates
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Chronic endometritis modifies decidualization in human endometrial stromal cells
Authors: D Wu, F Kimura, L Zheng, M Ishida, Y Niwa, K Hirata, A Takebayash, A Takashima, K Takahashi, R Kushima, G Zhang, T Murakami
Reprod. Biol. Endocrinol, 2017-03-04;15(1):16.
Species: Human
Sample Types: Cell Culture Supernates
FAQs
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Assay Diluent RD1-14 contains visible precipitate. Is this acceptable to use?
A small amount of precipitate is expected and acceptable in Assay Diluent RD1-14. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.
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