Human FRA-1 Antibody

Catalog #: MAB4935 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4935
MAB4935-SP

Detection of Human FRA‑1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FRA-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FRA-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FRA-2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 496106

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FRA-1
Gly146-Ala247
Accession # P15407

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FRA‑1 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 18 hours. PVDF Membrane was probed with 1 µg/mL of Human FRA-1 Monoclonal Antibody (Catalog # MAB4935) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FRA-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FRA-1

FRA-1 (FOSL1) is a 271 amino acid member of the leucine zipper Fos family of transcription factors. Fos family members have been associated with cellular proliferation, differentiation and transformation. In accordance with these findings, overexpression of FRA-1 correlates with a malignant phenotype in breast cancer. In lung epithelia cells, FRA-1 promotes motility, invasion, and anchorage independent growth. FRA-1 knock out mice rescued for the FRA-1 placental defect are viable but show reduced bone formation that may be due to a defect in the osteoblast differentiation. Human FRA-1 and mouse FRA-1 are 94% identical between amino acids 146-247.

Long Name

Fos-related antigen 1

Entrez Gene IDs

8061 (Human); 14283 (Mouse); 25445 (Rat)

Alternate Names

FOSL1; FOS-like antigen 1; FOS-like antigen-1; fos-related antigen 1; FRA; FRA1; FRA-1