Human FPRP/PTGFRN Antibody

Catalog #: MAB100431
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB100431-100
MAB100431-SP

Detection of FPRP/PTGFRN in U87-MG Human Cell Line by Flow Cytometry

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All FPRP/PTGFRN Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FPRP/PTGFRN Antibody Summary

Species Reactivity

Human

Specificity

Detects human FPRP/PTGFRN in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 998107

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human FPRP/PTGFRN
Pro27-Pro832
Accession # Q9P2B2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of FPRP/PTGFRN in U87-MG Human Cell Line by Flow Cytometry U87-MG human glioblastoma cell line was stained with Mouse Anti-Human FPRP/PTGFRN Monoclonal Antibody (Catalog # MAB100431, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FPRP/PTGFRN

FPRP (F_{2 alpha}Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F_{2 alpha} receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton.

Long Name

Prostaglandin F2-alpha Receptor Regulatory Protein

Entrez Gene IDs

5738 (Human); 19221 (Mouse); 29602 (Rat)

Alternate Names

CD315; CD9P-1; FPRP; PTGFRN

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human FPRP/PTGFRN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1