Human FOXL2 Antibody

Name: Human FOXL2 Antibody
Brand: R&D Systems
SKU: MAB10396
Rating: 5 (1 reviews)

Catalog #: MAB10396 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10396-100
MAB10396-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FOXL2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FOXL2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1019104

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human FOXL2
Accession # P58012

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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FOXL2 in PANC-1 Human Cell Line. FOXL2 was detected in immersion fixed PANC-1 human pancreatic carcinoma cell line (positive) and MCF-7 human breast cancer cell line (negative) using Mouse Anti-Human FOXL2 Monoclonal Antibody (Catalog # MAB10396) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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FOXL2 in Ovarian Cancer Tissue. FOXL2 was detected in immersion fixed paraffin-embedded sections of ovarian cancer tissue using Mouse Anti-Human FOXL2 Monoclonal Antibody (Catalog # MAB10396) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOXL2

FOXL2 is a forkhead transcription factor which plays a role in ovarian development and function. It is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary. Females missing the FOXL2 gene appear to be male. FOXL2 prevents transdifferentiation of an adult ovary to a testis. Overexpression of FOXL2 has been implicated in endometriosis with 3-fold higher mRNA expression in endometriosis than in healthy endometrium.

Long Name

Forkhead Box L2

Entrez Gene IDs

668 (Human); 26927 (Mouse)

Alternate Names

BPES; BPES1; BPES1PINTO; forkhead box L2; forkhead box protein L2; forkhead transcription factor FOXL2; FOXL2; PFRK; PINTO; POF3