Human FGFR1 (IIIb) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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FGF R1 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R1 Antibody. Recombinant Human FGF R1a (IIIb) Fc Chimera (Catalog # 655-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R1a (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R1 (IIIb) Monoclonal Antibody (Catalog # MAB765). The ND50 is typically 0.075-0.3 µg/mL in the presence of heparin (10 µg/mL).
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Detection of Human FGFR1 by Immunocytochemistry/Immunofluorescence Proximity between AcSDKP and FGFR1 inhibits the TGF beta /smad signaling pathway in HMVECs. (a) HMVECs were treated with N-FGFR1 (1.5 μg/ml) for 48 h with or without preincubation with AcSDKP (100 nM) for 2 h, and the proximity between AcSDKP and FGFR1 was analyzed by the Duolink In Situ Assay. For each slide, images at a × 400 original magnification were obtained from six different areas. (b and c) HMVECs were treated with TGF beta 2 (5 ng/ml) for 15 min or 48 h with or without preincubation with AcSDKP for 2 h, and the p-smad3, TGF beta R1, TGF beta R2 and FGFR1 levels were analyzed by western blot. Densitometric analysis of the p-smad3/smad3, TGF beta R1/ beta -actin, TGF beta R2/ beta -actin and FGFR1/ beta -actin levels from each group (n=6) were analyzed. (d and e) HMVECs were incubated with TGF beta 2 for 15 min or 48 h with or without preincubation with AcSDKP or its mutants (AcDSPK, AcSDKA, AcADKP) (100 nM) for 2 h. The p-smad3/smad3, TGF beta R1/ beta -actin, TGF beta R2/ beta -actin and FGFR1/ beta -actin protein levels were analyzed by western blot Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28771231), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGFR1
FGF R1 occurs as multiple isoforms. The extracellular domain consists of three ( alpha isoforms) or two ( beta isoforms) immunoglobulin-like domains. In addition, the membrane-proximal Ig-like domain can be encoded by alternative exons yielding (IIIb) or (IIIc) isoforms.
Product Datasheets
Citations for Human FGFR1 (IIIb) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
8
Citations: Showing 1 - 8
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Beta klotho is essential for the anti-endothelial mesenchymal transition effects of N-acetyl-seryl-aspartyl-lysyl-proline
Authors: Gao R, Kanasaki K, Li J et al.
FEBS Open Bio.
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Fibroblast growth factor receptor is a mechanistic link between visceral adiposity and cancer
Authors: D Chakrabort, V Benham, B Bullard, T Kearney, HC Hsia, D Gibbon, EY Demireva, SY Lunt, JJ Bernard
Oncogene, 2017-08-07;36(48):6668-6679.
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FGFR1 is critical for the anti-endothelial mesenchymal transition effect of N-acetyl-seryl-aspartyl-lysyl-proline via induction of the MAP4K4 pathway.
Authors: Li J, Shi S, et al.
Cell Death Dis
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Tissue transglutaminase promotes PDGF/PDGFR-mediated signaling and responses in vascular smooth muscle cells
Authors: Evgeny A. Zemskov, Irina Mikhailenko, Elizabeth P. Smith, Alexey M. Belkin
Journal of Cellular Physiology
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Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
Stem Cells, 2012-07-01;30(7):1455-64.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase.
Authors: Zemskov EA, Loukinova E, Mikhailenko I, Coleman RA, Strickland DK, Belkin AM
J. Biol. Chem., 2009-04-22;284(24):16693-703.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The Interaction of Apelin and FGFR1 Ameliorated the Kidney Fibrosis through Suppression of TGFbeta-Induced Endothelial-to-Mesenchymal Transition
Authors: R Gao, Y Wu, Q Yang, L Chen, J Chen, B Wang, Z Liu, J Jin, J Li, G Wu
Oxidative Medicine and Cellular Longevity, 2023-02-04;2023(0):5012474.
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Targeting the heparin-binding domain of fibroblast growth factor receptor 1 as a potential cancer therapy
Authors: Ling Ling, Si Kee Tan, Ting Hwee Goh, Edwin Cheung, Victor Nurcombe, Andre J. van Wijnen et al.
Molecular Cancer
FAQs
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Why is heparin necessary in the neutralization assay using Human FGFR1 (IIIb) Antibody, Catalog # MAB765?
The heparin is a necessary cofactor for the binding of FGF to the receptor which induces the proliferation that this antibody neutralizes.
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