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Human FGF-9 Antibody

Catalog #: MAB273
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB273-SP
MAB273-100
MAB273-500
Cell Proliferation Induced by FGF‑9 and Neutralization by Human FGF‑9 Antibody.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF-9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF-9 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) FGF-4, 5, 6, 7, 16, rhFGF acidic, or rhFGF basic is observed.
Source
Monoclonal Mouse IgG2A Clone # 36912
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF-9
Met1-Ser208
Accession # P31371
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample

Human FGF-9 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human FGF‑9 Biotinylated Antibody (Catalog # BAF273)

Standard: Recombinant Human FGF-9 Protein (Catalog # 273-F9)

Neutralization
Measured by its ability to neutralize FGF‑9-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.1-0.6 µg/mL in the presence of 10 ng/mL Recombinant Human FGF‑9.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by FGF‑9 and Neutralization by Human FGF‑9 Antibody. View Larger

Cell Proliferation Induced by FGF‑9 and Neutralization by Human FGF‑9 Antibody. Recombinant Human FGF-9 (Catalog # 273-F9) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-9 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF-9 Monoclonal Antibody (Catalog # MAB273). The ND50 is typically 0.1-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-9

The FGF family is comprised of at least nine polypeptides that show a variety of biological activities toward cells of mesenchymal, neuronal and epithelial origin. All FGFs have two conserved cysteine residues and share 30‑50% sequence identity at the amino acid level. FGF-9, also named glia-activating factor, was originally identified and purified from the supernatant of a human glioma cell line as a heparin-binding mitogenic growth factor for glial cells. FGF-9 has also been shown to stimulate the proliferation of oligodendrocyte type 2 astrocyte progenitor cells, Balb/c3T3 fibroblasts and PC-12 cells. However, unlike FGF acidic and basic, FGF-9 is not a mitogen for human umbilical vein endothelial cells.

The human FGF-9 cDNA encodes a 208 amino acid residue protein that contains a potential N-linked glycosylation site. The native protein is glycosylated. FGF-9 exhibits approximately 30% sequence similarity to other members of the FGF family. Although FGF-9 lacks a typical secretion signal, the protein is secreted efficiently after synthesis. Rat FGF-9 cDNA has been cloned and shown to be highly homologous to human FGF-9. The two proteins differ only in one amino acid residue. The expression of the FGF-9 transcripts has been shown to be restricted to the brain and the kidney.

References
  1. Naruo, K. et al. (1993) J. Biol. Chem. 268:2857.
  2. Miyamoto, M. et al. (1993) Mol. Cell Biol. 13:4251.
Long Name
Fibroblast Growth Factor 9
Entrez Gene IDs
2254 (Human); 14180 (Mouse)
Alternate Names
FGF9; FGF-9; fibroblast growth factor 9 (glia-activating factor); Fibroblast growth factor 9; GAF; glia-activating factor; HBFG-9; HBGF-9; Heparin-binding growth factor 9; MGC119914; MGC119915; SYNS3

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Citations for Human FGF-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms.
    Authors: Haichuan H, Zofia P, Patricia H et al.
    Cancer Cell.
  2. FGF9 from cancer-associated fibroblasts is a possible mediator of invasion and anti-apoptosis of gastric cancer cells.
    Authors: Sun C, Fukui H, Hara K, Zhang X, Kitayama Y, Eda H, Tomita T, Oshima T, Kikuchi S, Watari J, Sasako M, Miwa H
    BMC Cancer, 2015-04-30;15(0):333.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Fibroblast growth factor 9 has oncogenic activity and is a downstream target of Wnt signaling in ovarian endometrioid adenocarcinomas.
    Authors: Hendrix ND, Wu R, Kuick R, Schwartz DR, Fearon ER, Cho KR
    Cancer Res., 2006-02-01;66(3):1354-62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Expression and possible function of fibroblast growth factor 9 (FGF9) and its cognate receptors FGFR2 and FGFR3 in postnatal and adult retina.
    Authors: Cinaroglu A, Ozmen Y, Ozdemir A, Ozcan F, Ergorul C, Cayirlioglu P, Hicks D, Bugra K
    J. Neurosci. Res., 2005-02-01;79(3):329-39.
    Species: Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC-P, Neutralization
  5. Fgf9 induces proliferation and nuclear localization of FGFR2 in Sertoli precursors during male sex determination.
    Authors: Schmahl J, Kim Y, Colvin JS, Ornitz DM, Capel B
    Development, 2004-06-30;131(15):3627-36.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: IHC
  6. Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms.
    Authors: Haichuan H, Zofia P, Patricia H et al.
    Cancer Cell.

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