Human FGF-17 Antibody

Catalog #: AF319 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF319
AF319-SP

Cell Proliferation Induced by FGF‑17 and Neutralization by Human FGF‑17 Antibody.

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Human FGF-17 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FGF-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) FGF-8b and recombinant mouse FGF-8c is observed and less than 2% cross-reactivity with rhFGF acidic, rhFGF basic, rhFGF‑4, rhFGF‑5, rhFGF‑6, rhFGF‑7, rhFGF‑10, and rhFGF‑18 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FGF-17
Thr23-Thr216
Accession # O60258

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human FGF‑17 (Catalog # 319-FG)

Neutralization

Measured by its ability to neutralize FGF‑17-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND₅₀) is typically 6-24 µg/mL in the presence of 100 ng/mL Recombinant Human FGF‑17 and 1 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by FGF‑17 and Neutralization by Human FGF‑17 Antibody. Recombinant Human FGF‑17 (Catalog # 319-FG) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑17 (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF319). The ND₅₀ is typically 6-24 µg/mL in the presence of heparin (1 µg/mL).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-17

Fibroblast growth factors (FGFs) play multiple biological functions including angiogenesis, mitogenesis, cellular differentiation and wound repairing. All members of the FGF family have a conserved approximately 120 amino acid core with 30‑70% identity. Among FGF family members, FGF-17 is most similar to FGF-8 (60% sequence identity) and FGF-18 (50% sequence identity). The mRNA of FGF-17 was found in midgestation of embryo and multiple adult tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries. Human FGF‑17 shares 98.6% amino acid (aa) sequence identity with mouse FGF‑17. Rat FGF‑17 shares 100% aa sequence identity with mouse FGF‑17.

References