Human FATP1 Antibody

Catalog #: MAB3304
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3304
MAB3304-SP

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All FATP1 Antibodies

Product Details

Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FATP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FATP1 in cell transfectants but not non-transfected parent cells in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 308420

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human FATP1
Met1-Leu646
Accession # Q6PCB7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

HT‑29 human colon adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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FATP1 in HeLa Human Cell Line. FATP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human FATP1 Monoclonal Antibody (Catalog # MAB3304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FATP1

FATP1 is a plasma membrane protein with 6 transmembrane domains. It is involved in translocation of long-chain fatty acids across the plasma membrane. FATP1 is expressed in muscle, intestine, and adipose tissue.

Long Name

Fatty Acid Transport Protein 1

Entrez Gene IDs

376497 (Human); 26457 (Mouse); 94172 (Rat)

Alternate Names

ACSVL5FATP-1; EC 6.2.1; EC 6.2.1.-; FATP1; FATP1Solute carrier family 27 member 1; FATPlong-chain fatty acid transport protein 1; Fatty acid transport protein 1; FLJ00336; MGC71751; SLC27A1; solute carrier family 27 (fatty acid transporter), member 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human FATP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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The Phenotype of the Adipocytes Derived from Subcutaneous and Visceral ADMSCs Is Altered When They Originate from Morbidly Obese Women: Is There a Memory Effect?
Authors: A Mik?osz, B ?ukaszuk, E Supruniuk, K Grubczak, A Starosz, M Kusaczuk, M Naumowicz, A Chabowski
Cells, 2022-04-23;11(9):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment
Authors: M Bou, JS Torgersen, TK Østbye, B Ruyter, X Wang, S Škugor, IØ Kristianse, M Todor?evi?
Int J Mol Sci, 2020-06-08;21(11):.
Species: Fish - Atlantic Salmon
Sample Types: Whole Cells
Applications: ICC
Unraveling FATP1, regulated by ER-beta, as a targeted breast cancer innovative therapy
Authors: C Mendes, F Lopes-Coel, C Ramos, F Martins, I Santos, A Rodrigues, F Silva, S André, J Serpa
Sci Rep, 2019-10-01;9(1):14107.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot
The blood-brain barrier fatty acid transport protein 1 (FATP1/SLC27A1) supplies docosahexaenoic acid to the brain, and insulin facilitates transport
Authors: Yusuke Ochiai
J. Neurochem, 2017-03-30;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC