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Human ENPP-2/Autotaxin Antibody

Catalog #: AF5255
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5255
AF5255-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ENPP-2/Autotaxin Antibody Summary

Species Reactivity
Human
Specificity
Detects human ENPP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) ENPP‑5 and rhENPP‑7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ENPP-2/Autotaxin
Asp49-Ile863
Accession # Q13822
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ENPP‑2/Autotaxin (Catalog # 5255-EN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ENPP‑2/Autotaxin (Catalog # 5255-EN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ENPP-2/Autotaxin

ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40-50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain (1). Unlike ENPP-1 and ENPP-3, ENPP-2 has weak activity against nucleotides, but exhibits a lysophospholipase D activity which allows the formation of lysophosphatidic acid (LPA) and choline from lysophosphatidylcholine (2). The hydrolysis of nucleotides and lysophospholipids by ENPP-2 is mediated by a single catalytic site (2). Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2 (3). ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis (4) and to be up-regulated in several types of carcinomas including breast and lung (5).

References
  1. Cimpean, A. et al. (2004) Biochem. J. 381:71.
  2. Gijsbers, R. et al. (2003) FEBS Letters. 538:60.
  3. Van Meeteren, L.A. et al. (2005) J. Biol. Chem. 280:21155.
  4. Nam, S.W. et al. (2000) Oncogene 19:241.
  5. Jansen, S. et al. (2005) J. Cell Sci. 118:3081.
Long Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase 2
Entrez Gene IDs
5168 (Human); 18606 (Mouse); 84050 (Rat)
Alternate Names
ATX; ATXFLJ26803; ATX-X; Autotaxin; autotaxin-t; EC 3.1.4.39; ectonucleotide pyrophosphatase/phosphodiesterase 2; ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; ENPP2; ENPP-2; Extracellular lysophospholipase D; Lysophosphatidic Acid; LysoPLD; NPP2; PD-IALPHA; PDNP2; PDNP2NPP2; phosphodiesterase I/nucleotide pyrophosphatase 2; plasma lysophospholipase D

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Product Specific Notices

This product may be covered by one or more of the following US issued patents 5,449,753; 5,731,167; 6,084,069; 6,417,338 and US pending applications.

Citations for Human ENPP-2/Autotaxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity
    Authors: Lauren B. Rodda, Erick Lu, Mariko L. Bennett, Caroline L. Sokol, Xiaoming Wang, Sanjiv A. Luther et al.
    Immunity
  2. The polybasic insertion in autotaxin alpha confers specific binding to heparin and cell surface heparan sulfate proteoglycans.
    Authors: Houben A, van Wijk X, van Meeteren L, van Zeijl L, van de Westerlo E, Hausmann J, Fish A, Perrakis A, van Kuppevelt T, Moolenaar W
    J Biol Chem, 2012-11-13;288(1):510-9.
    Species: Human
    Sample Types: Recombinant Protein, Whole Cells
    Applications: ELISA Development, Flow Cytometry

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