Human Dynactin Subunit 2/DCTN2 Antibody

Catalog #: MAB6850 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6850
MAB6850-SP

Detection of Human Dynactin Subunit 2/DCTN2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dynactin Subunit 2/DCTN2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Dynactin Subunit 2/DCTN2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human
Dynactin Subunit 1/DCTN1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 696206

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Dynactin
Subunit 2/DCTN2
Asp277-Lys401
Accession # Q13561

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Dynactin Subunit 2/DCTN2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Dynactin Subunit 2/DCTN2 Monoclonal Antibody (Catalog # MAB6850) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Dynactin Subunit 2/DCTN2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Dynactin Subunit 2/DCTN2 in HeLa Human Cell Line. Dynactin Subunit 2/DCTN2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Dynactin Subunit 2/DCTN2 Monoclonal Antibody (Catalog # MAB6850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue. Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dynactin Subunit 2/DCTN2

DCTN2 (Dynactin-2; also 50 kDa Dynein-associated polypeptide and p50 Dynamitin) is a 50 kDa intracellular member of the Dynactin family of phosphoproteins. It is ubiquitously expressed and serves to stabilize the Dynactin complex of 11 different proteins. This complex is an obligate cofactor of Dynein and Kinesin motors, which position the mitotic spindle for cell division and move vesicles within the cell. Human Dynactin Subunit 2 is 401 amino acids (aa) in length. It contains three coiled‑coil regions (aa 99-132, 214-244 and 379-399), two potential phosphorylation sites (Ser83 and Tyr86), and one acetylation site at Ala2. The first two coiled‑coil regions contribute to oligomerization, while aa 1-91 are essential for Dynactin regulation. Potential isoform variants utilize either a five or two aa insertion after Ala35, an alternative start site at Met88, and a 21 aa substitution for aa 118-401. Over aa 277-401, human Dynactin Subunit 2 shares 95% aa identity with mouse Dynactin Subunit 2.

Entrez Gene IDs

10540 (Human); 69654 (Mouse); 299850 (Rat)

Alternate Names

DCTN2; DCTN-50; DCTN5050 kD dynein-associated polypeptide; dynactin 2 (p50); dynactin complex 50 kD subunit; Dynactin complex 50 kDa subunit; Dynactin Subunit 2; dynactin subunit 2,50 kDa dynein-associated polypeptide; Dynamitin; p50 dynamitin; RBP50