Home / DNAM-1/CD226 / Human DNAM-1/CD226 PE-conjugated Antibody

Human DNAM-1/CD226 PE-conjugated Antibody

Catalog #: FAB666P
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB666P
Detection of DNAM-1/CD226 in Human PBMCs by Flow Cytometry.
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Citations (4)
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Human DNAM-1/CD226 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DNAM-1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 102511
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DNAM-1
Glu19-Asn247 (predicted)
Accession # Q15762
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DNAM-1/CD226 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of DNAM-1/CD226 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3-cells were stained with Mouse Anti-Human DNAM-1/CD226 PE-conjugated Monoclonal Antibody (Catalog # FAB666P) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2‑6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts (7‑10). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (11). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 12‑14).

References
  1. Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359.
  2. Shibuya, A. et al. (1996) Immunity 4:573.
  3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
  5. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
  6. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
  9. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
  10. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
  11. Morimoto, K. et al. (2007) Oncogene July 16 epub.
  12. Shibuya, K. et al. (1999) Immunity 11:615.
  13. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
  14. Shibuya, A. et al. (1998) J. Immunol. 166:1671.
Long Name
DNAX Accessory Molecule 1
Entrez Gene IDs
10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
Alternate Names
CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1

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Citations for Human DNAM-1/CD226 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Expanded and activated allogeneic NK cells are cytotoxic against B-chronic lymphocytic leukemia (B-CLL) cells with sporadic cases of resistance
    Authors: T Calvo, C Reina-Orti, D Giraldos, M Gascón, D Woods, J Asenjo, J Marco-Brua, G Azaceta, I Izquierdo, L Palomera, D Sánchez-Ma, I Marzo, J Naval, C Vilches, M Villalba, A Anel
    Sci Rep, 2020-11-10;10(1):19398.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo
    Authors: M Boieri, A Ulvmoen, A Sudworth, C Lendrem, M Collin, AM Dickinson, L Kveberg, M Inngjerdin
    Oncoimmunology, 2017-01-17;6(3):e1274478.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Dopamine inhibits the effector functions of activated NK cells via the upregulation of the D5 receptor.
    Authors: Mikulak J, Bozzo L, Roberto A, Pontarini E, Tentorio P, Hudspeth K, Lugli E, Mavilio D
    J Immunol, 2014-08-15;193(6):2792-800.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The decreased expression of Siglec-7 represents an early marker of dysfunctional natural killer-cell subsets associated with high levels of HIV-1 viremia.
    Authors: Brunetta E, Fogli M, Varchetta S, Bozzo L, Hudspeth KL, Marcenaro E, Moretta A, Mavilio D
    Blood, 2009-08-26;114(18):3822-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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