Human Dermatopontin Antibody Summary
Gln19-Val201
Accession # AAH33736
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Dermatopontin by Western Blot. Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 1 µg/mL of Human Dermatopontin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4629) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dermatopontin at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dermatopontin
Dermatopontin, also known as TRAMP (tyrosine rich acidic matrix protein), is a widely expressed noncollagenous protein component of the extracellular matrix (1, 2). Mature human Dermatopontin shares 96%, 92%, and 92% amino acid sequence identity with bovine, mouse, and rat Dermatopontin, respectively. It is a 22 kDa molecule that is tyrosine sulfated but not glycosylated (3, 4). Dermatopontin contains three disulfide bonded loop structures that enclose conserved hexapeptide motifs (5). It accelerates collagen fibril formation in vitro, and Dermatopontin deficient mice exhibit altered collagen matrix deposition and organization (6 - 8). Dermatopontin is downregulated in fibrotic growths such as leiomyoma and scar tissue (9, 10). It binds both TGF-beta and the proteoglycan decorin, interactions that can increase the bioavailability of TGF-beta (11, 12). Dermatopontin promotes bone mineralization under the control of the vitamin D receptor and inhibits BMP-2 effects on osteoblast precursors (13, 14).
- Okamoto, O. and S. Fujiwara (2006) Connect. Tissue Res. 47:177.
- Superti-Furga, A. et al. (1993) Genomics 17:463.
- Forbes, E.G. et al. (1994) FEBS Lett. 351:433.
- Cronshaw, A.D. et al. (1993) Matrix 13:255.
- Neame, P.J. et al. (1989) J. Biol. Chem. 264:5474.
- MacBeath, J.R.E. et al. (1993) J. Biol. Chem. 268:19826.
- Takeda, U. et al. (2002) J. Invest. Dermatol. 119:678.
- Cooper, L.J. et al. (2006) Invest. Opthalmol. Vis. Sci. 47:3303.
- Catherino, W.H. et al. (2004) Genes Chromosomes Cancer 40:204.
- Kuroda, K. et al. (1999) J. Invest. Dermatol. 112:706.
- Okamoto, O. et al. (1996) J. Biochem. 119:106.
- Okamoto, O. et al. (1999) Biochem. J. 337:537.
- Pochampally, R.R. et al. (2007) J. Bone Miner. Res. 22:1338.
- Behnam, K. et al. (2006) Connect. Tissue Res. 47:271.
Product Datasheets
Citation for Human Dermatopontin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Highly concentrated trehalose induces prohealing senescence-like state in fibroblasts via CDKN1A/p21
Authors: Jun Muto, Shinji Fukuda, Kenji Watanabe, Xiuju Dai, Teruko Tsuda, Takeshi Kiyoi et al.
Communications Biology
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