Human Dectin-2/CLEC6A Antibody

Catalog #: MAB31141 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB31141-100
MAB31141-SP

Detection of Dectin‑2/CLEC6A in Human PBMC Monocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dectin-2/CLEC6A Antibody Summary

Species Reactivity

Human

Specificity

Detects human Dectin-2/CLEC6A in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 545925

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Dectin‑2/CLEC6A
Thr46-Leu209
Accession # Q6EIG7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Dectin‑2/CLEC6A in Human PBMC Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with (A) Mouse Anti-Human Dectin-2/CLEC6A Monoclonal Antibody (Catalog # MAB31141) or (B) Mouse IgG2b Isotype Control (Catalog # MAB0041) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-2/CLEC6A

Dectin-2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, plus CLEC4A-E constitute a subgroup of molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD), and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature human Dectin-2 is a type II transmembrane protein with a short cytoplasmic tail, a transmembrane segment, and a 168 aa ECD with a stalk region and one CRD (3, 4). Within the ECD, human Dectin-2 shares 71% and 75% aa sequence identity with bovine and mouse Dectin-2, respectively. An alternately spliced beta isoform has a deletion of portions of the transmembrane and cytoplasmic regions (5). Full length Dectin-2 is a 27 kDa molecule that is expressed on monocytes, tissue macrophages, and activated CD4⁺ T cells (4‑6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man₉GlcNAc₂ configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit on macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 is up‑regulated in vivo by inflammatory stimuli and UV-B irradiation (5, 6, 9). Dectin-2 is known to participate in UV-induced immunosuppression by interacting with CD4⁺CD25⁺ regulatory T cells, which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (10).

References

Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
Kanazawa, N. et al. (2004) Immunobiology 209:179.
Flornes, L.M. et al. (2004) Immunogenetics 56:506.
Kanazawa, N. et al. (2004) J. Invest. Dermatol. 122:1522.
Gavino, A.C. et al. (2005) Exp. Dermatol. 14:281.
Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
McGreal, E.P. et al. (2006) Glycobiology 16:422.
Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
Aragane, Y. et al. (2003) J. Immunol. 171:3801.

Entrez Gene IDs

93978 (Human); 56620 (Mouse)

Alternate Names

CLEC4N; CLEC6A; CLECSF10; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10; C-type lectin domain family 6 member A; C-type lectin domain family 6, member A; C-type lectin superfamily member 10; DC-associated C-type lectin 2; dectin 2; Dectin2; Dectin-2; Dendritic cell-associated C-type lectin 2