Human DARC PE-conjugated Antibody

Catalog #: FAB4139P
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB4139P

Detection of DARC in Human Red Blood Cells by Flow Cytometry.

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Citations (2)

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Human DARC PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DARC. Stains human DARC transfected cells but not irrelevant transfectants.

Source

Monoclonal Mouse IgG2a Clone # 358307

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of DARC in Human Red Blood Cells by Flow Cytometry. Human red blood cells were stained with Mouse Anti-Human DARC PE-conjugated Monoclonal Antibody (Catalog # FAB4139P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DARC

Duffy Antigen Receptor for Chemokines (DARC), also known as Fy glycoprotein and CD234, is a 7TM non-signaling chemokine receptor that is expressed on venular endothelial cells and erythrocytes. DARC binds and mediates the internalization of several inflammatory CC and CXC chemokines. It transports chemokines to the lumenal face of venular endothelium to promote the extravasation of leukocytes to sites of inflammation. DARC polymorphisms are important in blood transfusion compatibility and determine the susceptibility of erythrocytes to Plasmodium vivax infection. Human DARC shares 60% amino acid sequence identity with mouse DARC.

Long Name

Duffy Antigen, Receptor For Chemokines

Entrez Gene IDs

2532 (Human); 13349 (Mouse)

Alternate Names

ACKR1; CCBP1; CD234 antigen; CD234; DARC; Dfy; Duffy Antigen; Duffy antigen/chemokine receptor; Duffy blood group antigen; Duffy blood group, chemokine receptor; Fy Antigen; Fy glycoprotein; FYDuffy blood group; Glycoprotein D; GPD; GPDWBCQ1; GpFy; Plasmodium vivax receptor

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human DARC PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Altered activation state of circulating neutrophils in patients with neovascular age-related macular degeneration
Authors: Marie Krogh Nielsen, Sven Magnus Hector, Kelly Allen, Yousif Subhi, Torben Lykke Sørensen
Immunity & Ageing
Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors.
Authors: Huang, Xiaosong, Shah, Siddhart, Wang, Jing, Ye, Zhaohui, Dowey, Sarah N, Tsang, Kit Man, Mendelsohn, Laurel G, Kato, Gregory, Kickler, Thomas S, Cheng, Linzhao
Mol Ther, 2013-09-03;22(2):451-63.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry