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Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody

Catalog #: MAB5545
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MAB5545
MAB5545-SP
Detection of Human Cytosolic Sulfotransferase 1E1/SULT1E1 by Western Blot.
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Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cytosolic Sulfotransferase 1E1/SULT1E1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human SULT1A1, 1B1, 2A1, 2B1, or 4A1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 726211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Cytosolic Sulfotransferase 1E1/SULT1E1
Asn2-Ile294
Accession # P49888
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Cytosolic Sulfotransferase 1E1/SULT1E1 antibody by Western Blot. View Larger

Detection of Human Cytosolic Sulfotransferase 1E1/SULT1E1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cytosolic Sulfotransferase 1E1/SULT1E1 Monoclonal Antibody (Catalog # MAB5545) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 1E1/SULT1E1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 1E1/SULT1E1

Cytosolic Sulfotransferases catalyze the sulfation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1E1 is widely known as an estrogen sulfotransferase and may control estrogen levels by converting free estradiol to its inactive sulfate conjugate (1). Known substrates of this enzyme also include dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol, 4‑nitrophenol, 1-napthol and resveratrol (2, 3). SULT1E1 activity is found in reproductive organs (4), intestine and liver (3, 5).

References
  1. Gong, H. et al. (2008) Cancer Res. 68:7386.
  2. Falany, C.N. et al. (1995) J. Steroid Biochem. Mol. Biol. 52:529.
  3. Miksits, M. et al. 2005, Xenobiotica 35:1101.
  4. Takase, Y. et al. (2007) The Prostate 67:405.
  5. Schrag, M.L. et al. (2004) Drug Metabolism and Disposition 32:1299.
  6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
  7. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.
  8. Wu, Z.L., et al. (2002) Faseb J. 16:539.
    9.  
Entrez Gene IDs
6783 (Human); 20860 (Mouse); 25355 (Rat)
Alternate Names
Cytosolic Sulfotransferase 1E1; EC 2.8.2; EST-1; ESTMGC34459; estrone sulfotransferase; ST1E1; STE; STEestrogen sulfotransferase; Sulfotransferase 1E1; sulfotransferase family 1E, estrogen-preferring, member 1; Sulfotransferase, estrogen-preferringEC 2.8.2.4; SULT1E1

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Citation for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Capillary Western Immunoassay Optimization of Estrogen Related Factors in Human Subcutaneous Adipose Tissue
    Authors: JL Hill, KB McIver, K Katzer, MT Foster
    Methods and protocols, 2022-04-15;5(2):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Simple Western

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