Home / PD-L1/B7-H1 / Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit

Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit

Catalog #: DB7H10
21 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DB7H10
Control Products Available
QC226: Quantikine Immunoassay Control 1105 for h/cm PD-L1/B7-H1
Best Seller
Human PD-L1/B7-H1 ELISA Cell Culture Supernate/Cell Lysate/Serum/Plasma Standard Curve
3 Images
Product Details
Citations (21)
FAQs
Supplemental Products
Reviews (4)
Summary Sample Values Precision Recovery Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Cell Lysates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)
Sensitivity
4.52 pg/mL
Assay Range
25.0 - 1,600 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human B7-H1/PD-L1. This assay also recognizes natural cynomolgus monkey B7-H1/PD-L1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Sample TypeMean (pg/mL)Range (pg/mL)
Human Serum (n=36)62.544.5-106
Cynomolgus Monkey Serum (n=10)461248-953
Human EDTA plasma (n=36)56.239.1-108
Cynomolgus monkey EDTA plasma (n=10)20289.1-497
Human heparin plasma (n=36)52.233.4-110
Sample TypeMean of Detectable (pg/mL)Range (pg/mL)
Human Urine (n=10)34.5ND-34.5
ND=Non-detectable

Product Summary

The Quantikine® Human/Cynomolgus Monkey B7-H1/PD-L1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human/cynomolgus monkey B7-H1 in cell culture supernates, cell lysates, serum, plasma, and urine. It contains HEK293-expressed recombinant human B7-H1/PD-L1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human/cynomolgus monkey B7-H1/PD-L1 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human/cynomolgus monkey B7-H1/PD-L1.
Read this White Paper on detecting B7-H1/PD-L1 levels in multiple cancer subtypes.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2
n 20 20
Mean (pg/mL) 260 458
Standard Deviation 4.25 6.83
CV% 1.6 1.5

Recovery

The recovery of human/cynomolgus monkey B7-H1/PD-L1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 97-112
DYC002 (1X) (n=1) 96 90-100
EDTA Plasma (n=4) 90 79-100
Heparin Plasma (n=4) 85 76-95
Serum (n=4) 89 81-100
Urine (n=4) 109 100-116

Scientific Data

N/A Human PD-L1/B7-H1 ELISA Cell Culture Supernate/Cell Lysate/Serum/Plasma Standard Curve View Larger

Human PD-L1/B7-H1 ELISA Cell Culture Supernate/Cell Lysate/Serum/Plasma Standard Curve

N/A Human PD-L1/B7-H1 ELISA Urine Standard Curve View Larger

Human PD-L1/B7-H1 ELISA Urine Standard Curve

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PD-L1/B7-H1

PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells.

Long Name:
Programmed Death Ligand 1
Entrez Gene IDs:
29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names:
Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Human cytomegalovirus UL23 exploits PD-L1 inhibitory signaling pathway to evade T cell-mediated cytotoxicity
    Authors: Yuan, Q;Fan, Z;Huang, W;Huo, X;Yang, X;Ran, Y;Chen, J;Li, H;
    mBio
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Circulating tumour cells and PD-L1-positive small extracellular vesicles: the liquid biopsy combination for prognostic information in patients with metastatic non-small cell lung cancer
    Authors: Eslami-S Z, Cortés-Hernández LE, Sinoquet L et al.
    British journal of cancer
    Species: Human
    Sample Types: Extracellular Vesicle Lysates
  3. The Possible Clinical Significance of a Decreased Serum Level of Soluble PD-L1 in Discoid Lupus Erythematosus, but Not in Subacute Cutaneous Lupus Erythematosus-A Pilot Study
    Authors: Király, Z;Nagy, E;Bokor, L;Kovács, A;Marschalkó, M;Hidvégi, B;
    Journal of clinical medicine
    Species: Human
    Sample Types: Serum
  4. The screening of compounds regulating PD-L1 transcriptional activity in a cell functional high-throughput manner
    Authors: L Zhang, H Li, J Liu, G Sun, X Tang, S Xu, L Zhang, W Zhang, B Ai
    Cancer Medicine, 2023-03-25;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  5. Urine PD-L1 is a tumor tissue candidate substitute and is associated with poor survival in muscle-invasive bladder cancer patients
    Authors: J Ma, Q Zhou, W Xu, C Li, H Wang, Z Zhai, Y Zhang, W Wahafu
    International immunopharmacology, 2022-12-15;114(0):109535.
    Species: Human
    Sample Types: Urine
  6. High Pretreatment Serum PD-L1 Levels Are Associated with Muscle Invasion and Shorter Survival in Upper Tract Urothelial Carcinoma
    Authors: Á Széles, PT Kovács, A Csizmarik, M Váradi, P Riesz, T Fazekas, S Váncsa, P Hegyi, C Oláh, S Tschirdewa, C Darr, U Krafft, V Grünwald, B Hadaschik, O Horváth, P Nyirády, T Szarvas
    Biomedicines, 2022-10-13;10(10):.
    Species: Human
    Sample Types: Serum
  7. Active PD-L1 incorporation within HIV virions functionally impairs T follicular helper cells
    Authors: O Munoz, R Banga, R Schelling, FA Procopio, A Mastrangel, P Nortier, K Ohmiti, J Daraspe, M Cavassini, C Fenwick, L Perez, M Perreau
    PloS Pathogens, 2022-07-05;18(7):e1010673.
    Species: Human
    Sample Types: Plasma
  8. Circulating Levels of PD-L1, TIM-3 and MMP-7 Are Promising Biomarkers to Differentiate COVID-19 Patients That Require Invasive Mechanical Ventilation
    Authors: L Chavez-Gal, A Ruiz, K Martinez-E, H Aguilar-Du, M Torres, R Falfan-Val, G Pérez-Rubi, M Selman, I Buendia-Ro
    Biomolecules, 2022-03-14;12(3):.
    Species: Human
    Sample Types: Serum
  9. Exploring New Functional Aspects of HTLV-1 RNA-Binding Protein Rex: How Does Rex Control Viral Replication?
    Authors: K Nakano, K Yokoyama, S Shin, K Uchida, K Tsuji, M Tanaka, K Uchimaru, T Watanabe
    Viruses, 2022-02-16;14(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Soluble PD-L1 in Serum and Urine in Urinary Bladder Cancer Patients
    Authors: A Vikerfors, S Davidsson, J Frey, T Jerlström, J Carlsson
    Cancer, 2021-11-21;13(22):.
    Species: Human
    Sample Types: Serum
  11. Protein detection in blood with single-molecule imaging
    Authors: CP Mao, SC Wang, YP Su, SH Tseng, L He, AA Wu, RBS Roden, J Xiao, CF Hung
    Science Advances, 2021-08-11;7(33):.
    Species: Human
    Sample Types: Plasma
  12. Detection of PD-L1 in the urine of patients with urothelial carcinoma of the bladder
    Authors: G Tosev, W Wahafu, P Reimold, I Damgov, C Schwab, C Aksoy, A Kaczorowsk, A Stenzinger, J Nyarangi-D, M Hohenfelln, S Duensing
    Scientific Reports, 2021-07-09;11(1):14244.
    Species: Human
    Sample Types: Urine
  13. Serum levels of soluble programmed death-ligand 1 (sPD-L1): A possible biomarker in predicting post-treatment outcomes in patients with early hepatocellular carcinoma
    Authors: T Mocan, M Ilies, I Nenu, R Craciun, A Horhat, R Susa, I Minciuna, I Rusu, LP Mocan, A Seicean, CA Iuga, NA Hajjar, M Sparchez, DC Leucuta, Z Sparchez
    International immunopharmacology, 2021-02-18;94(0):107467.
    Species: Human
    Sample Types: Serum
  14. Soluble PD-L1 improved direct ARDS by reducing monocyte-derived macrophages
    Authors: J Xu, J Wang, X Wang, R Tan, X Qi, Z Liu, H Qu, T Pan, Q Zhan, Y Zuo, W Yang, J Liu
    Cell Death Dis, 2020-10-30;11(10):934.
    Species: Human
    Sample Types: Plasma
  15. Plasma soluble PD-L1 and STAT3 predict the prognosis in diffuse large B cell lymphoma patients
    Authors: Y Fei, J Yu, Y Li, L Li, S Zhou, T Zhang, L Li, L Qiu, B Meng, Y Pan, X Ren, Z Qian, X Wang, H Zhang
    J Cancer, 2020-10-17;11(23):7001-7008.
    Species: Human
    Sample Types: Plasma
  16. Activation of JUN in fibroblasts promotes pro-fibrotic programme and modulates protective immunity
    Authors: L Cui, SY Chen, T Lerbs, JW Lee, P Domizi, S Gordon, YH Kim, G Nolan, P Betancur, G Wernig
    Nat Commun, 2020-06-03;11(1):2795.
    Species: Human
    Sample Types: Bronchoalveolar lavage fluid
  17. Soluble PD-L1 in NSCLC Patients Treated with Checkpoint Inhibitors and Its Correlation with Metabolic Parameters
    Authors: A Castello, S Rossi, L Toschi, L Mansi, E Lopci
    Cancers (Basel), 2020-05-27;12(6):.
    Species: Human
    Sample Types: Plasma
  18. Circulating Levels of PD-L1 in Mesothelioma Patients from the NIBIT-MESO-1 Study: Correlation with Survival
    Authors: C Chiarucci, S Cannito, MG Daffinà, G Amato, G Giacobini, O Cutaia, MF Lofiego, C Fazio, D Giannarell, R Danielli, AM Di Giacomo, S Coral, L Calabrò, M Maio, A Covre
    Cancers (Basel), 2020-02-05;12(2):.
    Species: Human
    Sample Types: Serum
  19. Significance of PD-L1 in the diagnosis and treatment of B-cell malignant lymphoma
    Authors: J Yang, G Hu
    Oncol Lett, 2019-01-28;17(3):3382-3386.
    Species: Human
    Sample Types: Plasma
  20. Elevated serum soluble programmed cell death ligand 1 concentration as a potential marker for poor prognosis in small cell lung cancer patients with chemotherapy
    Authors: J Jin, J Si, Y Liu, H Wang, R Ni, J Wang
    Respir. Res., 2018-10-05;19(1):197.
    Species: Human
    Sample Types: Serum
  21. Clinical significance of soluble programmed cell death ligand-1 (sPD-L1) in hepatocellular carcinoma patients treated with radiotherapy
    Authors: HJ Kim, S Park, KJ Kim, J Seong
    Radiother Oncol, 2018-01-30;0(0):.
    Species: Human
    Sample Types: Plasma

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
Loading...

Reviews for Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit

Average Rating: 5 (Based on 4 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human PD-L1 Quantikine ELISA Kit
By Ritu Tripathi on 11/30/2018
Sample Tested: blood plasma
Human PD-L1 Quantikine ELISA Kit
By Anonymous on 10/10/2018
Sample Tested: Heparin Plasma
Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
By Leslie Priddy on 04/04/2018
Sample Tested: Serum
Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
By Anonymous on 08/02/2017
Sample Tested: A375 human melanoma cell line