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Human Common  gamma  Chain/IL-2 R gamma Antibody

Catalog #: MAB284
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB284-SP
MAB284-500
MAB284-100
Cell Proliferation Induced by IL‑2 and Neutralization by Human Common gamma Chain/ IL‑2 R gamma Antibody.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Common  gamma  Chain/IL-2 R gamma Antibody Summary

Species Reactivity
Human
Specificity
Detects human Common gamma Chain/IL‑2 R gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha, rhIL‑2 R beta, rhIL‑1 RI, rhIL‑1 RII, rhIL‑4 R, rhIL‑6 R, rhIL‑7 R, or rhIL‑10 R is observed.
Source
Monoclonal Mouse IgG1 Clone # 38024
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Common gamma Chain/IL‑2 R gamma
Leu23-Asn254
Accession # P31785
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IL‑2-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 2.5-15 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑2 and Neutralization by Human Common gamma Chain/ IL‑2 R gamma Antibody. View Larger

Cell Proliferation Induced by IL‑2 and Neutralization by Human Common gamma Chain/ IL‑2 R gamma Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-2 (30 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Human Common gamma Chain/IL-2 R gamma Monoclonal Antibody (Catalog # MAB284). The ND50 is typically 2.5-15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma  chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene.

Long Name
IL-2 Receptor gamma
Entrez Gene IDs
3561 (Human); 16186 (Mouse)
Alternate Names
CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency

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Citations for Human Common  gamma  Chain/IL-2 R gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. The involvement of interleukin-1 and interleukin-4 in the response of human annulus fibrosus cells to cyclic tensile strain: an altered mechanotransduction pathway with degeneration
    Authors: Hamish T J Gilbert, Judith A Hoyland, Anthony J Freemont, Sarah J Millward-Sadler
    Arthritis Research & Therapy
  2. Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
    Authors: Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
    J. Immunol., 2009-06-15;182(12):7389-97.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  3. Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
    Authors: Hirst SJ, Hallsworth MP, Peng Q
    Am. J. Respir. Crit. Care Med., 2002-04-15;165(8):1161-71.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  4. Cortactin and dynamin are required for the clathrin-independent endocytosis of gammac cytokine receptor.
    Authors: Sauvonnet N, Dujeancourt A, Dautry-Varsat A
    J. Cell Biol., 2004-12-28;168(1):155-63.

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