Human Collagen IV alpha 1 Antibody Summary
Ser1441-Thr1669
Accession # P02462
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Collagen IV alpha 1 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF Membrane was probed with 1 µg/mL of Human Collagen IV a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6308) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Collagen IV a1 at approximately 95 kDa (as indicated) ref 1. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Detection of Collagen IV alpha 1 in Human Kidney. Collagen IV alpha 1 was detected in immersion fixed paraffin-embedded sections of Human Kidney using Sheep Anti-Human Collagen IV alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6308) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen IV alpha 1
COL4A1 (collagen 4-alpha 1) is a calculated 185 kDa (ref. 1) member of the type IV collagen family. It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes, and endothelial cells. Two COL4A1 molecules interact with a 170 kDa alpha 2 chain to form a collagen IV triple helix. This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane. Mature human COL4A1 is 1642 amino acids (aa) in length (SwissProt #:P02462). It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C‑terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669). Multiple splice forms exist. One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958‑1669. Over aa 1441-1669, human COL4A1 shares 97% aa sequence identity to mouse COL4A1. AF6308 detects a 95 kDa band, endogenous COL4A1 by Westen blot in human placenta tissue sample, which is consistent with the current literature (ref. 1)
Product Datasheets
Citations for Human Collagen IV alpha 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Tetrahexyldecyl Ascorbate (THDC) Degrades Rapidly under Oxidative Stress but Can Be Stabilized by Acetyl Zingerone to Enhance Collagen Production and Antioxidant Effects
Authors: WR Swindell, M Randhawa, G Quijas, K Bojanowski, RK Chaudhuri
International Journal of Molecular Sciences, 2021-08-15;22(16):.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Capture -
Gelatinolytic activity of autocrine matrix metalloproteinase-9 leads to endothelial de-arrangement in Moyamoya disease
Authors: KG Blecharz-L, V Prinz, M Burek, D Frey, T Schenkel, SM Krug, M Fromm, P Vajkoczy
J. Cereb. Blood Flow Metab., 2018-04-10;0(0):271678X187684.
Species: Human
Sample Types: Tissue Lysates
Applications: Western Blot
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